AUTHOR=Zhou Yuqiao , Zhou Wei , Zhou Jinzhi , Yan Jinchang , Xu Dingting , Zheng Xiner , Zong Shuaizhou , Jiang Ping , Tian Shiyi , Han Jianzhong , Qu Daofeng 

TITLE=The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in Klebsiella

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.790673

DOI=10.3389/fmicb.2021.790673

ISSN=1664-302X

ABSTRACT=<p>Microorganisms have developed many strategies in the process of long-term defense against external attacks, one of which is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) bacterial immunological system. In this study, the whole genome of 300 strains of <italic>Klebsiella</italic> was collected, the CRISPR-Cas system in the strains was statistically analyzed, and the types and structures of CRISPR system in <italic>Klebsiella</italic> were explored, as well as the correlation between CRISPR and mobile genetic elements (MGEs). Through principal component analysis (PCA), we found that Cas gene, plasmids, integron, IS<italic>1</italic>, IS<italic>609</italic>, and enzymes of DNA metabolism were closely related to CRISPR-Cas. Compared the structural characteristics of plasmids, the DinG family helicases, Cas6, Csf2, and IS<italic>5</italic> were observed near the CRISPR loci in plasmid, which is also confirmed by the results of PCA that they may be important factors affecting the plasmid with CRISPR.</p>