AUTHOR=Pina-Pérez María Consuelo , Úbeda-Manzanaro María , Beyrer Michael , Martínez Antonio , Rodrigo Dolores 

TITLE=In vivo Assessment of Cold Atmospheric Pressure Plasma Technology on the Bioactivity of Spirulina

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.781871

DOI=10.3389/fmicb.2021.781871

ISSN=1664-302X

ABSTRACT=<p>The present study challenges the <italic>in vivo</italic> assessment of cold atmospheric pressure plasma (CAPP) technology on the bioactive activity (antioxidant/antiaging and antimicrobial potential) of <italic>Spirulina</italic> powder, using <italic>Caenorhabditis elegans</italic> as an animal model. Surface microdischarge cold atmospheric pressure plasma (SMD-CAPP) treatment was 3.3 W discharge power for 7 min. <italic>C. elegans</italic> lifespan and egg laying were used as indicators of antioxidant/antiaging potential of <italic>Spirulina</italic> (1 mg/mL), when grown with <italic>Spirulina</italic> CP-treated [E_SCP] and untreated [E_S], compared with a control [E_0] (non-supplemented with <italic>Spirulina</italic>). According to our results, under both <italic>Spirulina</italic> supplemented media [E_SCP and E_S] and for the first 17 days, nematodes experienced an increase in lifespan but without significant differences (<italic>p</italic> &gt; 0.05) between control and <italic>Spirulina</italic> CP-treated. Regarding the <italic>in vivo</italic> assay of the antimicrobial potential of <italic>Spirulina</italic> against <italic>Salmonella enterica</italic> serovar Typhimurium (infected worms), no significant differences (<italic>p</italic> &gt; 0.05) were found between the three exposure scenarios (control [S_0]; <italic>Spirulina</italic> supplemented media [S_S]; CP-treated <italic>Spirulina</italic> supplemented media [S_SCP]). According to present results, CAPP-treatment do not influence negatively the lifespan of <italic>C. elegans</italic> but a reduction in the S<italic>pirulina</italic> antiaging potential was found. No <italic>in vivo</italic> modifications in antimicrobial activity seem to be linked to CAPP-processed <italic>Spirulin</italic>a.</p>