AUTHOR=Liu Guorong , Nie Rong , Liu Yangshuo , Li Xue , Duan Jiaojiao , Hao Xu , Shan Yumeng , Zhang Jingying 

TITLE=Bacillus subtilis BS-15 Effectively Improves Plantaricin Production and the Regulatory Biosynthesis in Lactiplantibacillus plantarum RX-8

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.772546

DOI=10.3389/fmicb.2021.772546

ISSN=1664-302X

ABSTRACT=<p>Plantaricin is a broad-spectrum bacteriocin produced by <italic>Lactiplantibacillus plantarum</italic> with significant food industry application potential. It was found that the plantaricin production of <italic>L. plantarum</italic> RX-8 was enhanced when co-culturing with <italic>Bacillus subtilis</italic> BS-15. This study, therefore, set out to explore how <italic>B. subtilis</italic> BS-15 induces biosynthesis of plantaricin. The effect of co-culturing with <italic>B. subtilis</italic> BS-15 on cell growth, plantaricin production, quorum-sensing (QS) signal molecule PlnA/autoinducer-2 (AI-2) secretion, as well as plantaricin biosynthesis gene cluster and AI-2 synthesis-associated gene expression, was investigated in bacteriocin-producer <italic>L. plantarum</italic> RX-8. When <italic>L. plantarum</italic> RX-8 and <italic>B. subtilis</italic> BS-15 were co-inoculated in Man–Rogosa–Sharp (MRS) for 20 h at an inoculum ratio of 1:1 (10<sup>6</sup>:10<sup>6</sup> CFU/ml), the greatest plantaricin output (2,048 AU/ml) was obtained, rising by 32-fold compared with the monoculture of <italic>L. plantarum</italic> RX-8. Additionally, co-culture increased PlnA-inducing activity and AI-2 activity by 8- and 1.14-fold, respectively, over monoculture. RT-qPCR findings generated every 4 h (4–32 h) demonstrated that <italic>B. subtilis</italic> BS-15 remarkably improved the transcription of <italic>plnABCD</italic> and <italic>plnEF</italic>, and increased <italic>pfs</italic> and <italic>luxS</italic> transcription, even when using 200 mM D-ribose, a kind of AI-2 inhibitor. Based on the above findings, co-culturing with <italic>B. subtilis</italic> BS-15 as an environmental stimulus could activate the plantaricin induction <italic>via</italic> the PlnA-mediated intraspecies QS system and the AI-2-mediated interspecies QS system. Moreover, the inducing effect of PlnA and AI-2 in co-culture was independent. Differential proteomics analysis of <italic>B. subtilis</italic> BS-15 in co-culture indicated that bacteriocin-inducing regulatory mechanism may be related to flagellar assembly, peptidoglycan biosynthesis, anaerobic respiration, glycine cleavage system, or thiamin pyrophosphate biosynthesis.</p>