AUTHOR=Bastian Fabiola , Melayah Delphine , Hugoni Mylène , Dempsey Nora M. , Simonet Pascal , Frenea-Robin Marie , Fraissinet-Tachet Laurence 

TITLE=Eukaryotic Cell Capture by Amplified Magnetic in situ Hybridization Using Yeast as a Model

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.759478

DOI=10.3389/fmicb.2021.759478

ISSN=1664-302X

ABSTRACT=<p>A non-destructive approach based on magnetic <italic>in situ</italic> hybridization (MISH) and hybridization chain reaction (HCR) for the specific capture of eukaryotic cells has been developed. As a prerequisite, a HCR-MISH procedure initially used for tracking bacterial cells was here adapted for the first time to target eukaryotic cells using a universal eukaryotic probe, Euk-516R. Following labeling with superparamagnetic nanoparticles, cells from the model eukaryotic microorganism <italic>Saccharomyces cerevisiae</italic> were hybridized and isolated on a micro-magnet array. In addition, the eukaryotic cells were successfully targeted in an artificial mixture comprising bacterial cells, thus providing evidence that HCR-MISH is a promising technology to use for specific microeukaryote capture in complex microbial communities allowing their further morphological characterization. This new study opens great opportunities in ecological sciences, thus allowing the detection of specific cells in more complex cellular mixtures in the near future.</p>