AUTHOR=Cai Jianling , Pan Chaoming , Zhao Ye , Xu Hong , Tian Bing , Wang Liangyan , Hua Yuejin 

TITLE=DRJAMM Is Involved in the Oxidative Resistance in Deinococcus radiodurans

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.756867

DOI=10.3389/fmicb.2021.756867

ISSN=1664-302X

ABSTRACT=<p>Proteins containing JAB1/MPN/MOV34 metalloenzyme (JAMM/MPN<sup>+</sup>) domains that have Zn<sup>2+</sup>-dependent deubiquitinase (DUB) activity are ubiquitous across among all domains of life. Recently, a homolog in <italic>Deinococcus radiodurans</italic>, DRJAMM, was reported to possess the ability to cleave DRMoaD-MoaE. However, the detailed biochemical characteristics of DRJAMM <italic>in vitro</italic> and its biological mechanism <italic>in vivo</italic> remain unclear. Here, we show that DRJAMM has an efficient <italic>in vitro</italic> catalytic activity in the presence of Mn<sup>2+</sup>, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and Ni<sup>2+</sup> in addition to the well-reported Zn<sup>2+</sup>, and strong adaptability at a wide range of temperatures. Disruption of <italic>drJAMM</italic> led to elevated sensitivity in response to H<sub>2</sub>O<sub>2</sub><italic>in vivo</italic> compared to the wild-type R1. In particular, the expression level of MoaE, a product of DRJAMM cleavage, was also increased under H<sub>2</sub>O<sub>2</sub> stress, indicating that DRJAMM is needed in the antioxidant process. Moreover, DRJAMM was also demonstrated to be necessary for dimethyl sulfoxide respiratory system in <italic>D. radiodurans</italic>. These data suggest that DRJAMM plays key roles in the process of oxidative resistance in <italic>D. radiodurans</italic> with multiple-choice of metal ions and temperatures.</p>