AUTHOR=Guo Xiaoran , Zhao Kuan , Liu Xiaona , Lei Baishi , Zhang Wuchao , Li Xiuli , Yuan Wanzhe 

TITLE=Construction and Generation of a Recombinant Senecavirus a Stably Expressing the NanoLuc Luciferase for Quantitative Antiviral Assay

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.745502

DOI=10.3389/fmicb.2021.745502

ISSN=1664-302X

ABSTRACT=Senecavirus A (SVA), also known as Seneca Valley virus, is a recently emerged picornavirus that can cause swine vesicular disease, posing a great threat to the global swine industry. To rapidly detect anti-SVA neutralizing antibodies and establish a high-throughput screen for antiviral agents, a recombinant reporter virus (rSVA-Nluc) stably expressing the Nanoluciferase (Nluc) gene between SVA 2A and 2B was developed. This recombinant virus displays similar growth kinetics as the parental virus, and remained stable for more than 10 passages in BHK-21 cells. As a proof-of-concept for its utility for rapid antiviral screening, this reporter virus was used to rapidly quantify anti-SVA neutralizing antibodies in 13 swine sera samples, and to screen for antiviral agents, including interferons, ribavirin, and interferon-stimulated genes. Subsequently, interfering RNAs targeting different regions of the SVA genome were screened using the reporter virus. This reporter virus (rSVA-Nluc) represents a useful tool for rapid and quantitative screening and evaluation of antivirals against SVA.