AUTHOR=de Oliveira Marta Rodrigues , Katak Ricardo de Melo , da Silva Gilvan Ferreira , Marinotti Osvaldo , Terenius Olle , Tadei Wanderli Pedro , de Souza Afonso Duarte Leão , de Souza Antonia Queiroz Lima 

TITLE=Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.743246

DOI=10.3389/fmicb.2021.743246

ISSN=1664-302X

ABSTRACT=<p>The global increase in diseases transmitted by the vector <italic>Aedes aegypti</italic>, new and re-emerging, underscores the need for alternative and more effective methods of controlling mosquitoes. Our aim was to identify fungal strains from the Amazon rain forest that produce metabolites with larvicidal activity against <italic>Aedes aegypti</italic>. Thirty-six fungal strains belonging to 23 different genera of fungi, isolated from water samples collected in the state of Amazonas, Brazil were cultivated. The liquid medium was separated from the mycelium by filtration. Medium fractions were extracted with ethyl acetate and isopropanol 9:1 volume:volume, and the mycelia with ethyl acetate and methanol 1:1. The extracts were vacuum dried and the larvicidal activity was evaluated in selective bioassays containing 500 μg/ml of the dried fungal extracts. Larval mortality was evaluated up to 72 h. None of the mycelium extracts showed larvicidal activity greater than 50% at 72 h. In contrast, 15 culture medium extracts had larvicidal activity equal to or greater than 50% and eight killed more than 90% of the larvae within 72 h. These eight extracts from fungi belonging to seven different genera (<italic>Aspergillus, Cladosporium, Trichoderma, Diaporthe, Albifimbria, Emmia</italic>, and <italic>Sarocladium</italic>) were selected for the determination of LC<sub>50</sub> and LC<sub>90</sub>. <italic>Albifimbria lateralis</italic> (1160) medium extracts presented the lowest LC<sub>50</sub> value (0.268 μg/ml) after 24 h exposure. <italic>Diaporthe ueckerae</italic> (1203) medium extracts presented the lowest value of LC<sub>90</sub> (2.928 μg/ml) at 24 h, the lowest values of LC<sub>50</sub> (0.108 μg/ml) and LC<sub>90</sub> (0.894 μg/ml) at 48 h and also at 72 h (LC<sub>50</sub> = 0.062 μg/ml and LC<sub>90</sub> = 0.476 μg/ml). Extracts from <italic>Al. lateralis</italic> (1160) and <italic>D. ueckerae</italic> (1203) showed potential for developing new, naturally derived products, to be applied in integrated vector management programs against <italic>Ae. aegypti</italic>.</p>