AUTHOR=Wang Zhe , Zhang Chunxiao , Lu Kangle , Song Kai , Li Xueshan , Wang Ling , Rahimnejad Samad 

TITLE=Effects of Supplementing Intestinal Autochthonous Bacteria in Plant-Based Diets on Growth, Nutrient Digestibility, and Gut Health of Bullfrogs (Lithobates catesbeianus)

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.739572

DOI=10.3389/fmicb.2021.739572

ISSN=1664-302X

ABSTRACT=<p>Poor utilization efficiency of plant protein diets always leads to intestinal barrier dysfunction and growth inhibition in animals. Probiotics have shown promise in improving growth performance and gut health of the host. However, obtaining the host-beneficial probiotic from thousands of bacterial phylotypes is challenging. Here, four intestinal autochthonous bacteria were isolated from fast-growing bullfrog after a 60-day feeding on a soybean meal (SM)-based diet. Another feeding trial was conducted to evaluate the effects of supplementing these strains in an SM-based diet on growth, nutrient digestibility, immunity, and gut health of bullfrog. A high-SM basal diet was used as a non-supplemented control group (NC), and four other diets were prepared by supplementing the basal diet with 1 × 10<sup>7</sup> CFU/g of <italic>Bacillus siamensis</italic>, <italic>Bacillus tequilensis</italic> (BT), <italic>Bacillus velezensis</italic>, and <italic>Lactococcus lactis</italic> (LL). Results showed that weight gain, feed efficiency, nitrogen retention, and apparent digestibility coefficients of dry matter and protein were significantly higher in the LL group compared with the NC group (<italic>p</italic> &lt; 0.05). Furthermore, compared with the NC group, both BT and LL groups showed markedly higher jejunal protease and amylase activities, serum complement 4 and immunoglobulin M levels, jejunal muscularis thickness (<italic>p</italic> &lt; 0.05), and up-regulated expression of <italic>il-10</italic> and <italic>zo-1</italic> genes (<italic>p</italic> &lt; 0.05). High-throughput sequencing revealed higher abundances of <italic>Bacillus</italic> and <italic>Cetobacterium</italic> in BT and LL groups, respectively, accompanied with decreased abundances of <italic>Enterobacter</italic> and <italic>Escherichia–Shigella</italic>. Besides, KEGG pathways related to metabolisms were significantly enhanced by the LL diet relative to the NC diet (<italic>p</italic> &lt; 0.05). Overall, the beneficial effects of two frog-derived probiotics were determined: supplementation of <italic>L. lactis</italic> in SM-based diet promoted growth and nutrient digestibility; both <italic>B. tequilensis</italic> and <italic>L. lactis</italic> supplementation improved immune response and intestinal barrier function of bullfrogs.</p>