AUTHOR=Wang Jingang , Shan Qinghua , Ran Ye , Sun Dexiang , Zhang Haizhen , Zhang Jinzhu , Gong Shufang , Zhou Aimin , Qiao Kun 

TITLE=Molecular Characterization of a Tolerant Saline-Alkali Chlorella Phosphatidate Phosphatase That Confers NaCl and Sorbitol Tolerance

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.738282

DOI=10.3389/fmicb.2021.738282

ISSN=1664-302X

ABSTRACT=<p>The gene encoding a putative <italic>phosphatidate phosphatase</italic> (<italic>PAP</italic>) from tolerant saline-alkali (TSA) <italic>Chlorella</italic>, <italic>ChPAP</italic>, was identified from a yeast cDNA library constructed from TSA <italic>Chlorella</italic> after a NaCl treatment. <italic>ChPAP</italic> expressed in yeast enhanced its tolerance to NaCl and sorbitol. The ChPAP protein from a GFP-tagged construct localized to the plasma membrane and the lumen of vacuoles. The relative transcript levels of <italic>ChPAP</italic> in <italic>Chlorella</italic> cells were strongly induced by NaCl and sorbitol as assessed by northern blot analyses. Thus, ChPAP may play important roles in promoting Na-ion movement into the cell and maintaining the cytoplasmic ion balance. In addition, ChPAP may catalyze diacylglycerol pyrophosphate to phosphatidate in vacuoles.</p>