AUTHOR=Sun Bing , Luo Huaizhi , Jiang Huan , Wang Zhennan , Jia Aiqun 

TITLE=Inhibition of Quorum Sensing and Biofilm Formation of Esculetin on Aeromonas Hydrophila

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.737626

DOI=10.3389/fmicb.2021.737626

ISSN=1664-302X

ABSTRACT=<p>Quorum sensing (QS) and biofilm formation inhibition activity of esculetin on <italic>Aeromonas hydrophila</italic> SHAe 115 were evaluated. Exposure to esculetin at 25, 50, and 100μg/ml significantly inhibited the production of protease and hemolysin, the formation of biofilms and attenuated the swarming motility of <italic>A. hydrophila</italic> SHAe 115. Biofilm forming inhibition was also observed through confocal laser scanning microscopy and scanning electron microscope. Quantitative real-time PCR analysis indicated that genes positively related to QS and biofilm formation were downregulated to varying degrees, while gene (<italic>litR</italic>) negatively related to biofilm formation was significantly upregulated. The phenotypic results were in good agreement with gene expression levels. These results indicated that esculetin would be a potential QS inhibitor for <italic>A. hydrophila</italic>.</p>