AUTHOR=Yao Yuanyuan , Shu Xian , Wang Dongdong , Kan Wenjie , Su Pengfei , Hu Hao , Chen Xu , Wang Dacheng , Huang Shengwei , Wu Lifang 

TITLE=Non-enzymatic Transformation of Aflatoxin B1 by Pseudomonas geniculata m29

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.724103

DOI=10.3389/fmicb.2021.724103

ISSN=1664-302X

ABSTRACT=<p>Aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) is the most harmful mycotoxin produced by filamentous fungi and presents a serious threat to human and animal health. Therefore, it is essential to protect humans and animals from AFB<sub>1</sub>-induced acute and chronic toxicity. In this study, <italic>Pseudomonas</italic> strain m29 having a high efficiency of AFB<sub>1</sub> transformation was isolated from soil. The transformation ratio by m29 was more than 97% within 24 h, and the optimum temperature for transformation was 37°C. Moreover, the AFB<sub>1</sub> transforming activity was mainly attributed to the cell-free supernatant of strain m29. The metabolite that plays a crucial role in AFB<sub>1</sub> transformation is likely 1,2-dimethylhydrazine or 1,1-dimethylhydrazine, as identified by GC-MS and LC-MS analysis. AFB<sub>1</sub> was transformed into a product with molecular formula C<sub>17</sub>H<sub>14</sub>O<sub>7</sub>. To the best of our knowledge, this is the first study of non-enzymatic AFB<sub>1</sub> transformation by bacteria. Importantly, this AFB<sub>1</sub> transformation mechanism could be universal to various microorganisms.</p>