AUTHOR=Zhang Xiaoyan , Zhou Qian , Tang Mengjun , Pu Junhua , Zhang Jing , Lu Junxian , Zhang Yunzeng , Gao Yushi 

TITLE=Aminoglycoside Resistance and Possible Mechanisms in Campylobacter Spp. Isolated From Chicken and Swine in Jiangsu, China

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.716185

DOI=10.3389/fmicb.2021.716185

ISSN=1664-302X

ABSTRACT=<p><italic>Campylobacter</italic> is a major food-borne pathogen in humans, and previous studies reported a high prevalence of gentamicin-resistant <italic>Campylobacter</italic> isolates from food-producing animals in China. This study aimed to investigate the aminoglycoside resistance of <italic>Campylobacter</italic> isolated from chicken and swine in Jiangsu province, China and understand the possible mechanisms responsible for aminoglycoside resistance. One hundred and eighty-five <italic>Campylobacter</italic> isolates of chicken and swine origins in 2017 and 2018 were analyzed for gentamicin and kanamycin resistance. Some aminoglycoside resistance genes were selected for PCR detection in all strains. The genomic DNAs of two strains with high resistance to gentamicin were used as donors to subject <italic>C. jejuni</italic> NCTC11168 to natural transformation. The transformants were investigated by whole-genome sequencing and analyzed comparatively with <italic>C. jejuni</italic> NCTC11168. In total, 30.5% (29/95) of <italic>C. jejuni</italic> isolates and 42.2% (38/90) of <italic>C. coli</italic> isolates were resistant to gentamicin and kanamycin. The prevalence of the <italic>aph(2")-If</italic> gene and <italic>aac(6')-Ie/aph(2")-Ia</italic> gene was 65.4% (121/185) and 36.2% (67/185) in <italic>Campylobacter</italic> isolates, respectively. The <italic>aadE-sat4-aphA-3</italic> cluster was identified in 8.7% (8/92) and 20.4% (19/93) of all <italic>Campylobacter</italic> isolates in each year. With each donor DNA, aminoglycoside-resistant transformants were obtained. The transformants showed ≥128-fold increases in the MICs of gentamicin, kanamycin, and tobramycin. A 5200-bp segment was found to be inserted between the highly conserved genes <italic>Cj0299</italic> and <italic>panB</italic> of <italic>Campylobacter</italic>. A total of 9.7% (18/185) strains showing high resistance to aminoglycosides had this segment by PCR detection. The genetic diversity of the insertion-fragment positive strains was determined by MLST, and seven sequence types were identified for these strains.</p>