AUTHOR=Pan Guojun , Zhao Yanfen , Ren Shuang , Liu Fengyang , Xu Qicai , Pan Weibin , Yang Tongtao , Yang Mingtian , Zhang Xinru , Peng Chuanyue , Hao Gangping , Kong Fandong , Zhou Liman , Xiao Na 

TITLE=Indole-Terpenoids With Anti-inflammatory Activities From Penicillium sp. HFF16 Associated With the Rhizosphere Soil of Cynanchum bungei Decne

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.710364

DOI=10.3389/fmicb.2021.710364

ISSN=1664-302X

ABSTRACT=<p>Four new indole-terpenoids <bold>(1–4)</bold> named encindolene A, 18-<italic>O</italic>-methyl-encindolene A, encindolene B, and encindolene C, as well as three known analogs <bold>(5–7)</bold>, were isolated from the fungus <italic>Penicillium</italic> sp. HFF16 from the rhizosphere soil of <italic>Cynanchum bungei</italic> Decne. The structures of compounds including absolute configurations were elucidated by spectroscopic data and electronic circular dichroism (ECD) analysis. Anti-inflammatory activity evaluation revealed that compounds <bold>1–7</bold> inhibit the production of nitric oxide with IC<sub>50</sub> values of 79.4, 49.7, 81.3, 40.2, 86.7, 90.1, and 54.4 μM, respectively, and decrease the levels of tumor necrosis factor-α, interleukin-6 contents in lipopolysaccharide-induced RAW264.7 macrophages.</p>