AUTHOR=Song Wenya , Zhang Tengyue , Dong Jingyi , Luo Xiaotian , Bourland William A. , Wang Yurui 

TITLE=Taxonomy and Molecular Phylogeny of Two New Urostylid Ciliates (Protozoa: Ciliophora) From Chinese Wetlands and Establishment of a New Genus

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.707954

DOI=10.3389/fmicb.2021.707954

ISSN=1664-302X

ABSTRACT=<p>Hypotrich ciliates with evolutionary novelties are continually being discovered, challenging the current taxonomic system and attracting increased attention. In the present work, two new urostylid ciliates, <italic>Heterobakuella bergeri</italic> gen. nov., sp. nov. and <italic>Anteholosticha perezuzae</italic> sp. nov., isolated from Chinese wetland samples, were identified based on morphology and 18S rRNA gene sequences. <italic>Heterobakuella</italic> gen. nov. is defined by three frontal cirri, single buccal cirrus, one parabuccal cirrus, midventral complex composed of cirral pairs and one cirral row, one left and two right marginal cirral rows, transverse and pretransverse cirri present, caudal and frontoterminal cirri absent. <italic>Heterobakuella</italic> can be easily distinguished from the morphologically most similar genus, <italic>Apobakuella</italic>, mainly by the single buccal cirrus (vs. one buccal cirral row) and one parabuccal cirrus (vs. several parabuccal cirral rows originated from different anlagen). Phylogenetic analyses show that <italic>H. bergeri</italic> branches within the clade formed by <italic>Bergeriella ovata</italic>, <italic>Monocoronella carnea</italic>, <italic>Anteholosticha gracilis</italic>, and <italic>Neourostylopsis</italic> spp., rather than the clade represented by <italic>Apobakuella</italic>. The other species, <italic>A. perezuzae</italic>, is mainly characterized by a distinctly slender body shape with an average length:width ratio about 7, distinctively shaped biconcave and greenish cortical granules, as well as one or two pretransverse cirri. Phylogenetic analyses indicate the genus <italic>Anteholosticha</italic> is non-monophyletic.</p>