AUTHOR=Zhou Zhengyuan , Hu Ruiwen , Ni Yanmei , Zhuang Wei , Luo Zhiwen , Huang Weiming , Yan Qingyun , He Zhili , Zhong Qiuping , Wang Cheng 

TITLE=Genetic Elucidation of Quorum Sensing and Cobamide Biosynthesis in Divergent Bacterial-Fungal Associations Across the Soil-Mangrove Root Interface

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.698385

DOI=10.3389/fmicb.2021.698385

ISSN=1664-302X

ABSTRACT=<p>Plant roots in soil host a repertoire of bacteria and fungi, whose ecological interactions could improve their functions and plant performance. However, the potential microbial interactions and underlying mechanisms remain largely unknown across the soil-mangrove root interface. We herein analyzed microbial intra- and inter-domain network topologies, keystone taxa, and interaction-related genes across four compartments (non-rhizosphere, rhizosphere, episphere, and endosphere) from a soil-mangrove root continuum, using amplicon and metagenome sequencing technologies. We found that both intra- and inter-domain networks displayed notable differences in the structure and topology across four compartments. Compared to three peripheral compartments, the endosphere was a distinctive compartment harboring more dense co-occurrences with a higher average connectivity in bacterial-fungal network (2.986) than in bacterial (2.628) or fungal network (2.419), which could be related to three bacterial keystone taxa (<italic>Vibrio</italic>, <italic>Anaerolineae</italic>, and <italic>Desulfarculaceae</italic>) detected in the endosphere as they are known to intensify inter-domain associations with fungi and stimulate biofilm formation. In support of this finding, we also found that the genes involved in cell-cell communications by quorum sensing (<italic>rhlI</italic>, <italic>lasI</italic>, <italic>pqsH</italic>, and <italic>lasR</italic>) and aerobic cobamide biosynthesis (<italic>cobG</italic>, <italic>cobF</italic>, and <italic>cobA</italic>) were highly enriched in the endosphere, whereas anaerobic cobamide biosynthesis (encoded by <italic>cbiT</italic> and <italic>cbiE</italic>) was dominant in three peripheral compartments. Our results provide genetic evidence for the intensified bacterial-fungal associations of root endophytes, highlighting the critical role of the soil-root interface in structuring the microbial inter-domain associations.</p>