We aimed to optimize and validate the drug susceptibility test (DST) assay by SYBR Green I/PI (SG-PI) method using a panel of 89
By staining with SYBR Green I and PI dyes, green fluorescence and red fluorescence, which linearly correlated with the percentages of live and dead or membrane damaged cells, respectively, were used to produce two standard curves to calculate the relative cell membrane impermeable rates for each log and stationary phase cultures. Stationary phase
Data showed that after antibiotic treatment for 30–60 min, the antibiotic-resistant
We demonstrate that our novel SG-PI assay can accurately and stably detect resistance to different antibiotics in clinical isolates of