AUTHOR=Zhang Qian , Wang Shumin , Zhang Xinyu , Zhang Ruiling , Zhang Zhong 

TITLE=Negative Impact of Pseudomonas aeruginosa Y12 on Its Host Musca domestica

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.691158

DOI=10.3389/fmicb.2021.691158

ISSN=1664-302X

ABSTRACT=<p>High concentrations of <italic>Pseudomonas aeruginosa</italic> Y12 significantly inhibit the development of housefly larvae and accelerate larvae death. In this study, the dynamic distribution of the gut microbiota of housefly larvae fed different concentrations of <italic>P. aeruginosa</italic> Y12 was investigated. Compared with low-concentration <italic>P. aeruginosa</italic> diets, orally administered high-concentration <italic>P. aeruginosa</italic> diets caused higher mortality and had a greater impact on the community structure and interaction network of intestinal flora in housefly larvae. The bacterial community of the gut microbiota in housefly larvae was reconstructed in 4 days. Bacterial abundance and diversity were significantly reduced in housefly larvae fed high concentrations of <italic>P. aeruginosa</italic>. With the growth of larvae, the relative abundances of <italic>Providencia</italic>, <italic>Proteus</italic>, <italic>Myroides</italic>, <italic>Klebsiella</italic>, and <italic>Alcaligenes</italic> increased significantly in housefly larvae fed with high concentrations of <italic>P. aeruginosa</italic>, while the relative abundances of <italic>Bordetella</italic>, <italic>Enterobacter</italic>, <italic>Morganella</italic>, <italic>Ochrobactrum</italic>, <italic>Alcaligenaceae</italic>, and <italic>Empedobacter</italic> were significantly reduced. To analyze the role of the gut microorganisms played on housefly development, a total of 10 cultivable bacterial species belonging to 9 genera were isolated from the intestine of housefly larvae among which <italic>Enterobacter hormaechei</italic>, <italic>Klebsiella pneumoniae</italic>, <italic>Enterobacter cloacae</italic>, <italic>Lysinibacillus fusiformis</italic>, and <italic>Bacillus safensis</italic> promoted the growth of larvae through feeding experiments. This study is the first to analyze the influence of high concentrations of <italic>P. aeruginosa</italic> on the gut microbiota of houseflies. Our study provides a basis for exploring the pathogenic mechanism of high concentrations of <italic>P. aeruginosa</italic> Y12 in houseflies.</p>