AUTHOR=Guan Yu , Wang Kan , Zeng Yang , Ye Yanrui , Chen Ling , Huang Tengyi 

TITLE=Development of a Direct and Rapid Detection Method for Viable but Non-culturable State of Pediococcus acidilactici

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.687691

DOI=10.3389/fmicb.2021.687691

ISSN=1664-302X

ABSTRACT=<p><italic>Pediococcus acidilactici</italic> may significantly reduce the pH-value, and thus has different influence, including serving as a probiotic in human microbiota but a spoilage in human food as it could change the flavor. <italic>Pediococcus acidilactici</italic> is also capable of entering into the viable but non-culturable (VBNC) state causing false negative results of standard culture-based detection method. Thus, development of detection method for VBNC state <italic>P. acidilactici</italic> is of great significance. In this study, propidium monoazide (PMA) combined with cross priming amplification (CPA) was developed to detect the VBNC cells of <italic>P. acidilactici</italic> and applied on the detection in different systems. With detection limit of 10<sup>4</sup> cells/ml, high sensitivity, and 100% specificity, PMA-CPA can successfully detect VBNC cells of <italic>P. acidilactici</italic> and be applied in with high robustness.</p>