AUTHOR=Rai Anusha , Jagadeeshwari Uppada , Deepshikha Gupta , Smita Nandardhane , Sasikala Chintalapati , Ramana Chintalapati Venkata 

TITLE=Phylotaxogenomics for the Reappraisal of the Genus Roseomonas With the Creation of Six New Genera

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.677842

DOI=10.3389/fmicb.2021.677842

ISSN=1664-302X

ABSTRACT=<p>The genus <italic>Roseomonas</italic> is a significant group of bacteria which is invariably of great clinical and ecological importance. Previous studies have shown that the genus <italic>Roseomonas</italic> is polyphyletic in nature. Our present study focused on generating a lucid understanding of the phylogenetic framework for the re-evaluation and reclassification of the genus <italic>Roseomonas.</italic> Phylogenetic studies based on the 16S rRNA gene and 92 concatenated genes suggested that the genus is heterogeneous, forming seven major groups. Existing <italic>Roseomonas</italic> species were subjected to an array of genomic, phenotypic, and chemotaxonomic analyses in order to resolve the heterogeneity. Genomic similarity indices (<italic>d</italic>DDH and ANI) indicated that the members were well-defined at the species level. The Percentage of Conserved Proteins (POCP) and the average Amino Acid Identity (AAI) values between the groups of the genus <italic>Roseomonas</italic> and other interspersing members of the family <italic>Acetobacteraceae</italic> were below 65 and 70%, respectively. The pan-genome evaluation depicted that the pan-genome was an open type and the members shared 958 core genes. This claim of reclassification was equally supported by the phenotypic and chemotaxonomic differences between the groups. Thus, in this study, we propose to re-evaluate and reclassify the genus <italic>Roseomonas</italic> and propose six novel genera as <italic>Pararoseomonas</italic> gen. nov., <italic>Falsiroseomonas</italic> gen. nov., <italic>Paeniroseomonas</italic> gen. nov., <italic>Plastoroseomonas</italic> gen. nov., <italic>Neoroseomonas</italic> gen. nov., and <italic>Pseudoroseomonas</italic> gen. nov.</p>