AUTHOR=Kui Ling , Chen Baozheng , Chen Jian , Sharifi Rouhallah , Dong Yang , Zhang Zhanjiang , Miao Jianhua 

TITLE=A Comparative Analysis on the Structure and Function of the Panax notoginseng Rhizosphere Microbiome

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.673512

DOI=10.3389/fmicb.2021.673512

ISSN=1664-302X

ABSTRACT=<p><italic>Panax notoginseng</italic>, an important Chinese medicinal herb, can be mainly cultivated in two planting patterns, cropland planting (DT) and understory planting (LX). We speculate that the rhizosphere microbiome may vary in DT and LX and may play an important role in promoting the growth and health of <italic>P. notoginseng</italic>. In the present study, culture-independent Illumina HiSeq was employed to investigate the rhizosphere bacteria and fungi under DT and LX planting patterns. Predominant phyla include <italic>Proteobacteria</italic>, <italic>Acidobacteria</italic>, <italic>Actinobacteria</italic>, <italic>Gemmatimonadetes</italic>, and <italic>Ascomycota</italic> in the two planting patterns. DT has higher alpha diversity index than LX. The predominant LX-core genera include <italic>Bradyrhizobium, Streptomyces</italic>, <italic>and Actinomadura</italic>, and the predominant DT-core genera include <italic>Sphingomonas</italic>, <italic>Variovorax</italic>, and <italic>Novosphingobium</italic>. Total relative abundance of the disease-suppression phylum (<italic>Proteobacteria</italic>, <italic>Firmicutes</italic>, and <italic>Actinobacteria</italic>) and the potential plant growth-promoting rhizobacteria (PGPR) were both significantly higher in LX than in DT. We also identified over-presented microbial functional traits mediating plant–microbe and microbe–microbe interactions, nutrition acquisition, and plant growth promotion in <italic>P. notoginseng</italic> rhizosphere. Our findings provide a valuable reference for studying beneficial microbes and pathogens of <italic>P. notoginseng</italic> planted in DT and LX.</p>