AUTHOR=Lee Junho , Kang Hee Kyoung , Cheong Hyeonsook , Park Yoonkyung 

TITLE=A Novel Antimicrobial Peptides From Pine Needles of Pinus densiflora Sieb. et Zucc. Against Foodborne Bacteria

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.662462

DOI=10.3389/fmicb.2021.662462

ISSN=1664-302X

ABSTRACT=Pine needle is a traditional medicine used in several East Asian countries. It contains functional components that exhibit a wide spectrum of biological effects such as antioxidant and antimicrobial activities. We determined the antimicrobial activity of novel peptides isolated from Pinus densiflora Sieb. et Zucc. The four active pine-needle (PN) peptides showed potent antimicrobial activity against foodborne bacteria with minimum inhibitory concentration values ranged from 8 and 128 μg/mL. PN peptides showed no detectable hemolytic activity or cytotoxicity at the antimicrobial concentrations. The PN5 N-terminal amino acid sequence was identified using Edman degradation and BLAST homology analysis showed that it was not identical to any other protein. This suggests that PN5 can serve as an antimicrobial agent to be used as a preservative in the food industry.