AUTHOR=Zhang Honghai , Zhang Heng , Qin Xing , Wang Xiaolu , Wang Yuan , Bin Yao , Xie Xiangming , Zheng Fei , Luo Huiying 

TITLE=Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.658421

DOI=10.3389/fmicb.2021.658421

ISSN=1664-302X

ABSTRACT=<p>Deoxynivalenol (DON) is one of the most devastating and notorious contaminants in food and animal feed worldwide. A novel DON-degrading strain, <italic>Nocardioides</italic> sp. ZHH-013, which exhibited complete mineralization of DON, was isolated from soil samples. The intermediate products of DON generated by this strain were identified by high-performance liquid chromatography and ultra-performance liquid chromatography tandem mass spectrometry analyses. It was shown that, on an experimental level, 3-<italic>keto</italic>-DON was a necessary intermediate product during the conversion from DON to 3-<italic>epi</italic>-DON. Furthermore, the ZHH-013 strain could also utilize 3-<italic>epi</italic>-DON. This DON degradation pathway is a safety concern for food and feed. The mechanism of DON and 3-<italic>epi</italic>-DON elimination will be further studied, so that new enzymes for DON degradation can be identified.</p>