AUTHOR=Zhang Honghai , Wang Yuan , Brunecky Roman , Yao Bin , Xie Xiangming , Zheng Fei , Luo Huiying 

TITLE=A Swollenin From Talaromyces leycettanus JCM12802 Enhances Cellulase Hydrolysis Toward Various Substrates

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.658096

DOI=10.3389/fmicb.2021.658096

ISSN=1664-302X

ABSTRACT=<p>Swollenins exist within some fungal species and are candidate accessory proteins for the biodegradation of cellulosic substrates. Here, we describe the identification of a swollenin gene, <italic>Tlswo</italic>, in <italic>Talaromyces leycettanus</italic> JCM12802. <italic>Tlswo</italic> was successfully expressed in both <italic>Trichoderma reesei</italic> and <italic>Pichia pastoris</italic>. Assay results indicate that <italic>Tl</italic>SWO is capable of releasing reducing sugars from lichenan, barley β-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin. The specific activity of <italic>Tl</italic>SWO toward lichenan, barley β-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin is 9.0 ± 0.100, 8.9 ± 0.100, 2.3 ± 0.002 and 0.79 ± 0.002 U/mg, respectively. Additionally, <italic>Tl</italic>SWO had disruptive activity on Avicel and a synergistic effect with cellobiohydrolases, increasing the activity on pretreated corn stover by up to 72.2%. The functional diversity of <italic>Tl</italic>SWO broadens its applicability in experimental settings, and indicating that it may be a promising candidate for future industrial applications.</p>