AUTHOR=Li Xingguang , Liu Haizhou , Rife Magalis Brittany , Kosakovsky Pond Sergei L. , Volz Erik M. TITLE=Molecular Evolution of Human Norovirus GII.2 Clusters JOURNAL=Frontiers in Microbiology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.655567 DOI=10.3389/fmicb.2021.655567 ISSN=1664-302X ABSTRACT=Background

The human norovirus GII.2 outbreak during the 2016–2017 winter season was of unprecedented scale and geographic distribution.

Methods

We analyzed 519 complete VP1 gene sequences of the human norovirus GII.2 genotype sampled during the 2016–2017 winter season, as well as prior (dating back to 1976) from 7 countries. Phylodynamic analyses of these sequences were performed using maximum likelihood and Bayesian statistical frameworks in order to estimate viral evolutionary and population dynamics associated with the outbreak.

Results

Our results revealed an increase in the genetic diversity of human norovirus GII.2 during the recent Asian outbreak and diversification was characterized by at least eight distinct clusters. Bayesian estimation of viral population dynamics revealed a highly fluctuating effective population size, increasing in frequency during the past 15 years.

Conclusion

Despite an increasing viral diversity, we found no evidence of an elevated evolutionary rate or significant selection pressure in human norovirus GII.2, indicating viral evolutionary adaptation was not responsible for the volatility of or spread of the virus during this time.