AUTHOR=Wang Qingzhuo , Zhang Jiao , Al Makishah Naief H. , Sun Xiaoman , Wen Zhiqiang , Jiang Yu , Yang Sheng 

TITLE=Advances and Perspectives for Genome Editing Tools of Corynebacterium glutamicum

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.654058

DOI=10.3389/fmicb.2021.654058

ISSN=1664-302X

ABSTRACT=<p><italic>Corynebacterium glutamicum</italic> has been considered a promising synthetic biological platform for biomanufacturing and bioremediation. However, there are still some challenges in genetic manipulation of <italic>C. glutamicum</italic>. Recently, more and more genetic parts or elements (replicons, promoters, reporter genes, and selectable markers) have been mined, characterized, and applied. In addition, continuous improvement of classic molecular genetic manipulation techniques, such as allelic exchange via single/double-crossover, nuclease-mediated site-specific recombination, RecT-mediated single-chain recombination, actinophages integrase-mediated integration, and transposition mutation, has accelerated the molecular study of <italic>C. glutamicum.</italic> More importantly, emerging gene editing tools based on the CRISPR/Cas system is revolutionarily rewriting the pattern of genetic manipulation technology development for <italic>C. glutamicum</italic>, which made gene reprogramming, such as insertion, deletion, replacement, and point mutation, much more efficient and simpler. This review summarized the recent progress in molecular genetic manipulation technology development of <italic>C. glutamicum</italic> and discussed the bottlenecks and perspectives for future research of <italic>C. glutamicum</italic> as a distinctive microbial chassis.</p>