AUTHOR=Hua Xiaoting , Liang Qian , Deng Min , He Jintao , Wang Meixia , Hong Wenjie , Wu Jun , Lu Bian , Leptihn Sebastian , Yu Yunsong , Chen Huan 

TITLE=BacAnt: A Combination Annotation Server for Bacterial DNA Sequences to Identify Antibiotic Resistance Genes, Integrons, and Transposable Elements

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.649969

DOI=10.3389/fmicb.2021.649969

ISSN=1664-302X

ABSTRACT=<p>Whole genome sequencing (WGS) of bacteria has become a routine method in diagnostic laboratories. One of the clinically most useful advantages of WGS is the ability to predict antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in bacterial sequences. This allows comprehensive investigations of such genetic features but can also be used for epidemiological studies. A plethora of software programs have been developed for the detailed annotation of bacterial DNA sequences, such as rapid annotation using subsystem technology (RAST), Resfinder, ISfinder, INTEGRALL and The Transposon Registry. Unfortunately, to this day, a reliable annotation tool of the combination of ARGs and MGEs is not available, and the generation of genbank files requires much manual input. Here, we present a new webserver which allows the annotation of ARGs, integrons and transposable elements at the same time. The pipeline generates genbank files automatically, which are compatible with Easyfig for comparative genomic analysis. Our BacAnt code and standalone software package are available at <ext-link ext-link-type="uri" xlink:href="https://github.com/xthua/bacant" xmlns:xlink="http://www.w3.org/1999/xlink">https://github.com/xthua/bacant</ext-link> with an accompanying web application at <ext-link ext-link-type="uri" xlink:href="http://bacant.net" xmlns:xlink="http://www.w3.org/1999/xlink">http://bacant.net</ext-link>.</p>