AUTHOR=Li Qiang , Li Lijiao , Feng Huiyu , Tu Wenying , Bao Zhijie , Xiong Chuan , Wang Xu , Qing Yuan , Huang Wenli 

TITLE=Characterization of the Complete Mitochondrial Genome of Basidiomycete Yeast Hannaella oryzae: Intron Evolution, Gene Rearrangement, and Its Phylogeny

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.646567

DOI=10.3389/fmicb.2021.646567

ISSN=1664-302X

ABSTRACT=<p>In this study, the mitogenome of <italic>Hannaella oryzae</italic> was sequenced by next-generation sequencing (NGS) and successfully assembled. The <italic>H. oryzae</italic> mitogenome comprised circular DNA molecules with a total size of 26,444 bp. We found that the mitogenome of <italic>H. oryzae</italic> partially deleted the tRNA gene transferring cysteine. Comparative mitogenomic analyses showed that intronic regions were the main factors contributing to the size variations of mitogenomes in Tremellales. Introns of the <italic>cox1</italic> gene in Tremellales species were found to have undergone intron loss/gain events, and introns of the <italic>H. oryzae cox1</italic> gene may have different origins. Gene arrangement analysis revealed that <italic>H. oryzae</italic> contained a unique gene order different from other Tremellales species. Phylogenetic analysis based on a combined mitochondrial gene set resulted in identical and well-supported topologies, wherein <italic>H. oryzae</italic> was closely related to <italic>Tremella fuciformis</italic>. This study represents the first report of mitogenome for the <italic>Hannaella</italic> genus, which will allow further study of the population genetics, taxonomy, and evolutionary biology of this important phylloplane yeast and other related species.</p>