Skip to main content

REVIEW article

Front. Microbiol., 04 February 2021
Sec. Virology

Membrane Rafts: Portals for Viral Entry

\r\nIns Ripa,*Inés Ripa1,2*Sabina Andreu,Sabina Andreu1,2Jos Antonio Lpez-Guerrero,José Antonio López-Guerrero1,2Raquel Bello-Morales,Raquel Bello-Morales1,2
  • 1Departamento de Biología Molecular, Universidad Autónoma de Madrid, Madrid, Spain
  • 2Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Madrid, Spain

Membrane rafts are dynamic, small (10–200 nm) domains enriched with cholesterol and sphingolipids that compartmentalize cellular processes. Rafts participate in roles essential to the lifecycle of different viral families including virus entry, assembly and/or budding events. Rafts seem to participate in virus attachment and recruitment to the cell surface, as well as the endocytic and non-endocytic mechanisms some viruses use to enter host cells. In this review, we will introduce the specific role of rafts in viral entry and define cellular factors implied in the choice of one entry pathway over the others. Finally, we will summarize the most relevant information about raft participation in the entry process of enveloped and non-enveloped viruses.

Introduction

“Membrane rafts” or “lipid rafts” are small, dynamic membrane domains enriched with cholesterol and sphingolipids present in the plasma membrane, as well as in intracellular membranes and extracellular vesicles. Membrane rafts have the ability to concentrate or segregate specific elements in order to regulate their interactions with other components. Rafts may induce conformational changes in resident proteins, affecting their activity (Sezgin et al., 2017). Because of this, lipid rafts are essential for maintenance of cellular functions such as signal transduction (Koyama-Honda et al., 2020), receptor activation (Shi and Ruan, 2020), intracellular lipid and protein trafficking (Ouweneel et al., 2020), spatial organization of the plasma membrane (van IJzendoorn et al., 2020), endocytosis (Nichols, 2003a) and extracellular vesicle formation (Skryabin et al., 2020). As a consequence of their broad involvement in cell physiology, lipid rafts play an important role in complex processes including immune response (Varshney et al., 2016), host–pathogen interaction (Bukrinsky et al., 2020), cancer development (Greenlee et al., 2020), and cardiovascular disorders (Das and Das, 2009).

Regarding host–pathogen interactions, membrane rafts have been shown to play a role in viral life cycles, especially in processes like virus entry, assembly and/or budding (Chazal and Gerlier, 2003; Takahashi and Suzuki, 2009, 2011). Viruses are obligate intracellular parasites that must transport their genomes from infected cells to uninfected ones in order to initiate each new round of replication. To facilitate entry into cells, most viruses hijack cellular machinery, especially endocytic mechanisms. Only a few viruses are capable of directly penetrating the cell surface, crossing into the cytoplasm by fusing their envelope with the plasma membrane (Yamauchi and Helenius, 2013). Membrane rafts are implied in both endocytic mechanisms and viral entry via fusion (Figure 1). This review will focus on the involvement of rafts in the entry of enveloped (Table 1) and non-enveloped (Table 2) viruses, breaking down current studies in the field according to viral family.

FIGURE 1
www.frontiersin.org

Figure 1. Membrane raft structure. Lipid rafts are composed of cholesterol, saturated phospholipids and sphingolipids, such as glycolipids and sphingomyelin (SM). GPI-anchored proteins and lipidated – especially palmitoylated- proteins have a higher affinity for lipid rafts than non-lipid rafts.

TABLE 1
www.frontiersin.org

Table 1. Entry of enveloped viruses mediated by membrane rafts.

TABLE 2
www.frontiersin.org

Table 2. Entry of non-enveloped virus mediated by membrane rafts.

Composition of Membrane Rafts

Singer and Nicolson (1972) proposed one of the earliest models to accurately describe the structure of biological membranes, the “fluid mosaic model” of membranes. This model describes the cellular membrane as a uniform lipid bilayer with randomly distributed proteins. However, from the start it was observed that membranes are not uniform, since they are formed by clusters of lipids (Lee et al., 1974). The term “lipid domain” was established in 1982 by Karnovsky et al. (1982), who found that lipids have no homogeneity in their lateral distribution and that such organizational heterogeneity may have functional and structural significance.

A consensus definition for a membrane raft (Figure 1) was established at the 2006 Keystone Symposium of Lipid Rafts and Cell Function: “Membrane rafts are small (10–200 nm), heterogenous, highly dynamic, sterol- and sphingolipid-enriched domains that compartmentalize cellular processes. Small rafts can sometimes be stabilized to form larger platforms through protein-protein and protein-lipid interactions” (Pike, 2006). For membrane rafts, the most important interactions are between sterols, saturated phospholipids and sphingolipids, such as glycolipids and sphingomyelin (SM). Cholesterol and saturated lipids interact more strongly with each other than with unsaturated lipids. Because of this, in the presence of cholesterol, sphingolipids are condensed in a unique state of matter called ‘liquid ordered (Lo) phase’ (Figure 1). In this phase, lipid molecules have a high capacity for lateral diffusion, whereas the surrounding unsaturated lipids form the “liquid disordered (Ld) phase,” in which lipid molecules are mostly immobile (Levental et al., 2020). Two types of proteins are suggested to be associated with membrane rafts: glycosylphosphatidylinositol (GPI)-anchored proteins and lipidated -specifically palmitoylated- proteins (Sezgin et al., 2017). The raft affinity of transmembrane proteins is dependent on the physicochemical features -palmitoylation, length and surface area- of the transmembrane domains (TMDs), but generally tend to be excluded from lipid rafts (Lorent et al., 2017) (Figure 1).

It is important to keep in mind that most of the analyzed data are based on “detergent-resistant membranes” (DRMs) or “detergent-insoluble glycolipid-enriched membranes” (DIGs). The tight packing of the domains enriched in sphingolipids and cholesterol confers resistance to solubilization with non-ionic detergents (e.g., Triton X-100) at low temperatures (Graham, 2002; Chamberlain, 2004). Due to this property, membrane rafts were originally referred to as DRMs, which are enriched in sterols, sphingolipids and lipidated proteins, but are not exactly the same as membrane rafts. DRMs only exist after detergent treatment and may not necessarily correspond precisely with the native membrane structure present in live cells. The composition and properties of DRMs are dependent on the nature and concentration of the detergent, as well as the temperature and time of solubilization. On the other hand, membrane rafts are in vivo transient membrane microdomains, whose existence is independent from the use of detergents (Lichtenberg et al., 2005; Brown, 2006).

Dependence on Cholesterol or Sphingomyelin Does Not Imply Participation of Membrane Rafts in Viral Entry

The involvement of membrane rafts in viral entry is usually evaluated by the effects of raft-disrupting treatments, which mainly remove cholesterol from the plasma membrane or inhibit its synthesis. Inhibition of virus infection by cholesterol depletion is generally recoverable by the addition of exogenous cholesterol. The most commonly used raft-disrupting compound is methyl-β-cyclodextrin (MβCD), which extracts cholesterol from cells and rafts, abolishing association of raft proteins with DRMs and disrupting raft-regulated cell signaling pathways. However, MβCD not only extracts cholesterol from the plasma membrane, but also from intracellular compartments, thereby disrupting organelle function and structure as well as vesicular transport (Bieberich, 2018). Also, even though “raft-dependent pathways” are highly sensitive to cholesterol-depleting agents, clathrin-mediated endocytosis is also affected at high doses of MβCD (Rodal et al., 1999; Subtil et al., 1999). Thus, it is important to consider that cholesterol dependence may not necessarily prove the participation of intact rafts in viral entry.

Although less common, another experimental tool to analyze the importance of rafts in viral entry is the treatment of membranes with sphingomyelinases (SMases). SMases catalyze the hydrolysis of SM into ceramide, converting rafts into ceramide-rich platforms (CRPs). Although CRPs can be found in DRMs, ceramide no longer forms part of a raft with Lo structure, but rather forms its own lipid microdomain structure (Bieberich, 2018). Depletion of SM impairs the entry of, among others, pseudorabies virus (PRV) (Pastenkos et al., 2018), rubella virus (RuV) (Otsuki et al., 2017), Influenza virus A (IAV) (Audi et al., 2020), and hepatitis C virus (HCV) (Voisset et al., 2008) (Table 1). However, as happens with cholesterol, the requirement of SM for viral entry may not imply the participation of membrane rafts per se. For instance, the fusion peptide of Classical Swine Fever Virus (SFV) has a high affinity for cholesterol- and sphingolipid-enriched microdomains (Ahn et al., 2002), but using vesicles prepared from synthetic sphingolipids and sterols, it has been demonstrated that membrane rafts are not essential for the virus entry by fusion (Waarts et al., 2002). Throughout the review, we will find more examples about the use of cholesterol- and SM-depleting agents and the subsequent interpretation of the obtained results.

Membrane Rafts in Virus Attachment

Viral entry into a host cell is a complex process that first requires virus binding to the cell surface, often via a receptor. Viruses interact with different cell surface molecules that comprise a wide variety of proteins, lipids and glycans (Jolly and Sattentau, 2013; Bhella, 2015). The same cell receptor types can be recognized by different viruses, and one virus may be able to interact with more than one cell surface molecule. In some cases, interaction with a single receptor is sufficient to trigger infection, whereas in others it is necessary for the virus to interact with several receptor molecules (Sieczkarski and Whittaker, 2004).

The concentration of receptors, co-receptors and viral particles into rafts promotes activation of cell signaling pathways and enhances the efficiency of the entry process (Szklarczyk et al., 2013). One example is Coxsackie virus A9 (CVA9) infection (Table 2). The concentration of the receptor αvβ3, coreceptor GRP78 and MHC class I (which facilitates virus internalization) is increased within membrane rafts compared to uninfected controls. The production of molecules belonging to the Raf/MAPK pathway also increases during CVA9 infection. In this way, the entry and signaling machinery of the virus is concentrated in membrane rafts, which facilitates efficient viral entry (Triantafilou and Triantafilou, 2003).

Some cellular receptors and co-receptors are constitutively expressed in rafts, such as angiotensin-converting enzyme 2 (ACE2), receptor for severe acute respiratory syndrome coronavirus (SARS-CoV) (Glende et al., 2008; Lu et al., 2008) and SARS-CoV-2 (Hoffmann et al., 2020; Wang H. et al., 2020) (Table 1). In other cases, cell receptors and co-receptors are not constitutively located into rafts, but viral attachment to the cell surface triggers translocation to them. Such relocation of receptors and co-receptors into rafts has been observed during infection by human herpesvirus type 6 (HHV-6) (Tang et al., 2008), Kaposi’s sarcoma-associated herpesvirus (KSHV) (Chakraborty et al., 2011), vaccinia virus (Izmailyan et al., 2012; Schroeder et al., 2012), HIV-1 (Popik et al., 2002; Kamiyama et al., 2009) (Table 1), Coxsackie virus B4 (CVB4) (Triantafilou and Triantafilou, 2004) and enterovirus D68 (EV-D68) (Jiang et al., 2020) (Table 2). However, receptor recruitment into rafts is not always a consequence of a viral infection, although it exerts influence on virus entry. For example, translocation of the herpes simplex virus type 1 (HSV-1) receptor nectin-1 into rafts is induced by the presence of αVβ3-integrin at the plasma membrane, not by HSV-1 attachment to the cell surface (Gianni and Campadelli-Fiume, 2012) (Table 1).

The presence of viral particles and/or viral glycoproteins in membrane rafts during virus attachment is another way to demonstrate the involvement of rafts in entry process. For instance, a fraction of the glycoprotein gB of HSV-1 is associated with cell rafts from the moment of attachment and during entry (Bender et al., 2003) (Table 1). In some infections, such HIV-1 (Popik et al., 2002), HHV-6 (Tang et al., 2008) (Table 1) or echovirus type 71 (EV71) (Zhu et al., 2015) (Table 2), even the interaction of viral glycoprotein with the respective receptor within rafts have been shown. These studies strongly support a role for intact rafts in virus attachment, beyond the use of raft-disrupting agents.

However, although location of viral particles and/or cell receptors on membrane rafts gives us a lot of information about the entry pathway, it is important to consider that viruses may enter through a region different from the initial attachment site. Virus particles can bind to non-raft membranes, but then shift to raft domains. Vaccinia virus particles bind initially to glycosaminoglycans and laminin in non-raft domains, inducing further interactions with integrin β1 within rafts. The subsequent recruitment of the receptor CD98 into rafts and activation of downstream kinases lead to endocytosis of the virus (Izmailyan et al., 2012; Schroeder et al., 2012). In other cases, although viruses initially bind to a raft domain, they shift to another membrane domain for entry. For example, amphotropic murine leukemia virus (A-MLV) binds to large rafts without the protein caveolin-1, but then is transported into caveolae to enter the host cells (Beer and Pedersen, 2006) (Table 1).

Membrane Rafts in Endocytosis

Endocytosis is a cellular mechanism by which cells internalize substances from the external environment and can be classified into phagocytosis or pinocytosis. Phagocytosis is generally restricted to specialized cells, such as macrophages, and is typically employed to digest bacteria and/or large particles. Pinocytosis is a non-specific, non-saturable, and non-carrier-mediated form of membrane transport via vesicular uptake of fluids, macromolecules and small pathogens (Basturea, 2019). This last endocytic pathway, in turn, has been differentiated based on the coat proteins of the endocytic vesicle into: clathrin-mediated endocytosis and clathrin-independent endocytosis (Basturea, 2019).

Clathrin-mediated endocytosis (CME) (Kaksonen and Roux, 2018) is, to date, the most frequently known mechanism for endocytosis of small and medium-size viruses. After a period of lateral movement along the cell surface, receptor-bound virus particles enter into preexisting clathrin-coated pits (CCPs), which pinch off from the plasma membrane to form clathrin-coated vesicles (CCVs) that subsequently lose their clathrin coat and fuse with endosomes. This mechanism is dependent on dynamin-II, a GTPase required for detachment of coated vesicles from the plasma membrane (Singh et al., 2017).

Clathrin-independent endocytosis (CIE) pathways (Mayor et al., 2014; Sandvig et al., 2018; Shafaq-Zadah et al., 2020) are cholesterol-sensitive mechanisms that may be classified into caveolae-mediated endocytosis (dynamin-dependent) and non-clathrin non-caveolae mediated endocytosis, which can be dynamin-dependent (VanHamme et al., 2008) or independent (Damm et al., 2005). Caveolae (Bastiani and Parton, 2010; Cheng and Nichols, 2016) are the best characterized microdomains of membrane rafts, which consist of small plasma membrane invaginations at the surface of several mammalian cell types. They can bud into cells in the form of endocytic vesicles that merge to early endosomes for cargo delivery (Pelkmans et al., 2004). The inner layer of the caveolar coat is composed of caveolins, proteins with a hydrophobic transmembrane loop inside the membrane and both N- and C- termini facing the cytoplasm. There are three types of caveolins, Cav1, Cav2, and Cav3, the former being the major component of caveolae (Williams and Lisanti, 2004). Cav1 is a palmitoylated protein which uses cholesterol for binding to rafts and can influence numerous cellular processes by forming oligomers that interact with signaling molecules, regulate the cholesterol content of caveolae and lead to the formation of complex scaffold domains implied in caveolae biogenesis (Khater et al., 2019). On the other hand, the outer layer of the caveolar coat is composed by cavins (Briand et al., 2011), proteins that constitute homo- and hetero-oligomeric complexes which are thought to stabilize the caveolin scaffold and promote membrane curvature and budding of caveolae (Bastiani et al., 2009; McMahon et al., 2009; Kovtun et al., 2015).

In 2001, it was suggested that caveolar vesicles fuse with a newly discovered organelle called a “caveosome” (Pelkmans et al., 2001), characterized by a neutral pH and the presence of Cav1. However, in 2010 the same authors (Hayer et al., 2010a) clarified that caveosomes actually correspond to late endosomes modified by the accumulation of the overexpressed Cav1 awaiting degradation (Hayer et al., 2010b). Because of this, authors recommended that the term should no longer be used.

More recently, an alternative classification system has been established, taking into consideration the role of lipid rafts in endocytosis. Depending on membrane rafts, endocytic pathways may be classified into (i) pathways in which lipid rafts are not present in the endocytic vesicle -CME-; (ii) pathways for which endocytic vesicle can contain rafts and non-raft domains -phagocytosis and macropinocytosis-; and (iii) pathways that take place in membrane rafts – the majority of CIE-. The endocytic vesicles that are formed in lipid rafts can be stabilized by the enrichment of certain proteins, such as caveolin (caveolae-mediated endocytosis) or flotillin (flotillin-dependent endocytosis). Alternatively, endocytic vesicles can be formed into lipid rafts by the action of small guanosine triphosphatases (GTPasas), such as Cdc42 and Arf1 (GRAF1-dependent endocytosis), Arf6 (Arf6-dependent endocytosis) or RhoA (RhoA-dependent endocytosis) (El-Sayed and Harashima, 2013).

Viral Entry by Vesicle Formation in Membrane Rafts

Cell endocytic mechanisms can be exploited by viruses to enter host cells (Barrow et al., 2013; Cossart and Helenius, 2014). Receptor-virus attachment facilitate the concentration of viral particles and/or the activation of signaling pathways, promoting the membrane curvature and, as a consequence, viral endocytosis (Grove and Marsh, 2011). Subsequent penetration into the cytosol generally occurs through early or late endosomes, although additional penetration sites, such as the endoplasmic reticulum, are possible (Yamauchi and Helenius, 2013). The majority of both enveloped and naked viruses require a decrease in the pH of endocytic organelles in order to activate viral surface proteins involved in escape into the cytoplasm, prior to arrival in the degradative lysosome (Nicola, 2016).

Viral entry via endocytic vesicles can occur in raft domains of the plasma membrane. These raft-dependent pathways can be caveolae-mediated or clathrin- and caveolae- independent. Whereas caveolae-mediated endocytosis has been highly studied and reviewed in both enveloped and non-enveloped viruses (Xing et al., 2020), non-clathrin non-caveolae raft-dependent endocytosis is still poorly characterized. This novel mechanism was described for the first time in 2005 as an alternative entry pathway for Simian virus 40 (SV40) (Damm et al., 2005) (Table 2). Until 2005, it was believed that SV40 entered cells only by caveolae-mediated endocytosis (Figure 2). SV40 entry by caveolae has been extensively studied since 1996 (Anderson et al., 1996) and the entry of this virus is used as a control of caveolae-mediated endocytosis in several studies (Quirin et al., 2008). The new alternative pathway proposed for SV40 was not only caveolae independent, but also independent of clathrin, dynamin-II and Arf6 (a small GTPase involved in recycling pathway). However, it was cholesterol-sensitive and tyrosine kinase dependent. Thus, viral particles were associated with DRMs during the early stages of the viral cycle, revealing an involvement of intact lipid rafts (Damm et al., 2005).

FIGURE 2
www.frontiersin.org

Figure 2. (i) Simian virus 40 (SV40) entry by caveolae. SV40 binding to major histocompatibility class I (MHC-I) molecules targets the viral particles to lipid rafts, where multivalent binding to the ganglioside GM1 promotes viral entry by caveolae. Caveolae-mediated endocytosis can be exploited by other viruses, such as HCoC-229E, JEV or EV71. (ii) Adeno-associated virus type 2 (AAV2) entry by non-clathrin non-caveolae raft-dependent endocytosis. AAV2 enters the cells via clathrin-independent carrier/GPI-anchored protein early endosomal compartment (CLIC/GEEC). The formation of tubular vesicles by GRAF1-dependent endocytosis is mediated by the complementary roles of Cdc42 and Arf1 in regulating actin polymerization. Non-clathrin non-caveolae raft-dependent endocytosis can be also exploited by SV40 and SARS-CoV.

More recently, viral entry mediated by non-clathrin non-caveolae endocytosis in lipid rafts has been especially characterized in adeno-associated virus 2 (AAV2) (Table 2) infection (Figure 2). AAV2 particles enter host cells via GRAF1-dependent endocytosis, also known as CLIC/GEEC (clathrin-independent carrier/GPI-anchored protein early endosomal compartment) (Nonnenmacher and Weber, 2011). GEECs are vesicles derived from membrane rafts whose formation is independent of clathrin, caveolae, dynamin and Rac1. GEECs are formed from fusion of smaller CLICs. Actin polymerization -regulated by Cdc42 and Arf1- and GRAF1 protein drives the initial formation of CLICs and, consequently, GEEC generation (Shafaq-Zadah et al., 2020). The role of membrane rafts in viral entry by CLIC/GEEC has been proven by use of cholesterol-depleting reagents, the recovery of AAV2 from isolated membrane fractions enriched in raft markers, and by the physical association of virions and GRAF1-enriched vesicles in lipid rafts (Nonnenmacher and Weber, 2011).

In viruses such as human adenovirus species C (HAdv-C) (Colin et al., 2005), rhesus rotavirus (RRV) (Sánchez-San Martín et al., 2004) and EV1 (Krieger et al., 2013) the presence of non-clathrin non-caveolae cholesterol-sensitive endocytosis has been observed but, in contrast to SV40 or AAV2 entry, it has not been demonstrated to be raft-dependent. The non-clathrin non-caveolae pathway is especially controversial since it is sensitive to cholesterol depletion, but decreased viral entry caused by cholesterol-depleting reagents does not necessarily mean lipid raft involvement. One of the best examples of a virus that enters by a non-clathrin non-caveolae endocytosis which is cholesterol-sensitive but raft-independent is lymphocytic choriomeningitis virus (LCMV). Although cholesterol depletion with MβCD produced a reduction in LCMV infection (Rojek et al., 2008), the same MβCD concentrations they used inhibited several plasma membrane processes, including CME. After adjusting the concentration of raft-disrupting reagents to a lower level, sufficient to block the infection of the raft-dependent SV40 but without effects on CME of SFV, LCMV infection was no longer inhibited. Therefore, the authors proposed that LCMV entry is cholesterol-dependent but raft-independent (Quirin et al., 2008). A possible cause for this cholesterol sensitivity could be the association between the LCMV receptor – dystroglycan (DG) – with non-raft cholesterol, which is critical for LCMV infection (Shah et al., 2006).

Another example of raft-independent entry is the non-clathrin non-caveolae endocytosis of HIV-1 in polarized trophoblastic cells. Treatment with the cholesterol-sequestering drug filipin severely impaired virus internalization, whereas treatment with MβCD had no impact on this pathway. Part of the reduction in HIV-1 infectivity in the presence of filipin may be related to an indirect effect of the drug on HIV-1 gene expression. Collectively, authors concluded that the pathway requires free membrane cholesterol, and that membrane rafts appear to be involved at later points of virus entry process (Vidricaire and Tremblay, 2007).

Membrane Rafts in Viral Entry via Macropinocytosis

Macropinocytosis is a specialized form of CIE that is dependent upon cortical actin ruffling and results in the internalization of large amounts of fluid by enlarged vesicles, denominated macropinosomes (Donaldson, 2019). Regulatory factors of macropinocytosis include PAK-1, Arf6 and the Rho family GTPases, Rac1 and Cdc42. The main difference between phagocytosis and CME is dynamin-II independence (Mercer and Helenius, 2009). A relationship between macropinocytosis and lipid rafts has not been completely elucidated but cannot be ruled out. Macropinocytosis is sensitive to cholesterol-depletion and membrane ruffling may occur in raft domains. On the other hand, macropinosome formation requires vast areas of the plasma membrane. As a consequence, the final vesicle can be formed by a mixture of raft and non-raft domains (El-Sayed and Harashima, 2013).

Viruses can activate the signaling pathways that trigger macropinocytosis, promoting actin-mediated membrane ruffling and blebbing and the resulting macropinosome formation. Macropinocytosis has been linked to a requirement of cholesterol for viral entry. For instance, the entry of HIV-1 in the brain microvascular endothelia takes place by macropinocytosis, and is inhibited by cholesterol-extracting agents (Liu et al., 2002). As discussed before, a cholesterol requirement does not imply the involvement of rafts, but in certain infections by enveloped viruses a close relationship between macropinocytosis and membrane rafts per se has been demonstrated. For example, in Ebola virus (EBOV) infection (Table 1). Using fluorescently labeled Ebola virus like particles (VLPs), it is possible to visualize the dynamic internalization into live cells by macropinocytosis through membrane rafts (Jin et al., 2020).

The involvement of rafts in macropinocytosis has been especially studied in KSHV infection (Table 1 and Figure 3). Cholesterol depletion decreases KSHV infection and viral gene expression, suggesting that lipid rafts play a role in the entry and post binding stage (Raghu et al., 2007). Further research showed that the initial attachment of KSHV with heparan sulfate, α3β1, αVβ3, and αVβ5 integrins and the amino acid transporter x-CT occurs in non-raft domains (Veettil et al., 2014). The interaction of KSHV with these receptors induces the recruitment of the adaptor c-Cbl (Veetti et al., 2010), which is an E3 ubiquitin ligase which is able to influence cellular signal pathways by ubiquitinating target proteins to control their localization. c-Cbl selectively monoubiquitinates α3β1 and αVβ3 integrins, promoting the translocation of viral particles and receptors into membrane rafts at the junctional base of macropinocytic blebs (Chakraborty et al., 2011). In these raft domains, KSHV interacts with tyrosine kinase Ephrin A2 (EphA2) to amplify cascade signaling and to promote macropinosome formation (Chakraborty et al., 2012; Hahn et al., 2012). On the other hand, c-Cbl may polyubiquitinate αVβ5 integrin in non-lipid rafts, preventing its translocation into raft domains and inducing non-productive viral entry via clathrin (Chakraborty et al., 2011).

FIGURE 3
www.frontiersin.org

Figure 3. Kaposi’s sarcoma-associated herpesvirus (KSHV) entry by raft-dependent macropinocytosis in endothelial cells. Initial viral attachment occurs in non-lipid rafts. E3 ubiquitin ligase c-Cbl and membrane rafts determine the posterior location of receptors and the KSHV entry pathway. Polyubiquitination of the receptor αVβ5 leads to non-productive viral entry via clathrin in non-lipid rafts. Monoubiquitination of αVβ3 and α3β1 integrins induces the translocation of viral particles and receptors to lipid rafts, leading to productive viral entry via macropinocytosis. Viral entry by macropinocytosis in a raft-dependent manner has been also observed in EBOV infection.

These results show the relevance of membrane rafts in entry by macropinocytosis of certain viruses. Lipid rafts not only may constitute part of the vast area of plasma membrane required for macropinosome formation, but also could allow the clustering of viral particles and receptors and, as a consequence, the recruitment of signaling molecules necessary for macropinocytosis induction.

Relationship Between Membrane Rafts and Viral Entry by Clathrin

Initially, it seemed clear that CME was raft-independent. Some studies suggested the absence of rafts in CCPs (Nichols, 2003b), and there were general observations that rafts and raft-associated proteins internalize through CIE (Sato et al., 2004). However, membrane rafts can cooperate with clathrin in the internalization of some molecules, such as the B cell antigen receptor (BCR) (Stoddart et al., 2002) or certain GPI-anchored proteins (Rollason et al., 2007; Sarnataro et al., 2009).

Regarding viral infection, there are studies showing a dependence on both clathrin and cholesterol for cell entry, as in the case of varicella-zoster virus (VZV) (Hambleton et al., 2007), type C food-and-mouth- disease virus (FMDV) (Martín-Acebes et al., 2007), Crimea-Congo hemorrhagic fever virus (CCHFV) (Simon et al., 2009), Japanese encephalitis virus (JEV) (Yang et al., 2013) and HIV-1 (Praena et al., 2020). Besides, viruses internalized by clathrin may require cholesterol and/or SM to escape from the endocytic vesicle via fusion. This is the case of Semliki Forest virus (SFV) and Sindbis virus (SIN) (Phalen and Kielian, 1991; Nieva et al., 1994; Marquardt and Kielian, 1996; Smit et al., 1999). However, a cholesterol or SM requirement does not imply a direct relation between clathrin and membrane rafts per se.

The implication of intact membrane rafts in CME actually occurs at the virus attachment step. For example, cell surface attachment in hepatitis C virus (HCV) -via tetraspanin CD81 and scavenger receptor B type I (SR-BI) (Kapadia et al., 2007) - and the attachment of infectious bronchitis virus (IBV) (Guo et al., 2017) occurs in raft domains. However, after virus binding, entry of both HCV (Blanchard et al., 2006; Meertens et al., 2006; Helle and Dubuisson, 2008) and IBV (Wang et al., 2019a, b) are mediated by clathrin. One of the best examples of the possible relation between rafts and clathrin is the entry of JEV in neural stem cells. JEV infection is inhibited by cholesterol depletion, and viral envelope proteins (glycoprotein E and Nakayama) are associated with rafts in the early stage of infection. Glycoprotein E colocalizes with cholera toxin B (CTB), which enters cells in a raft-dependent manner, but can also be internalized by CME (Chinnapen et al., 2007; Day and Kenworthy, 2015). The possibility of caveolae-mediated endocytosis was raised, but the glycoprotein did not colocalize with Cav1. However, it colocalizes with transferrin – which is trafficked via clathrin (Mayle et al., 2012) – and clathrin-null mutants had reduced infection. Also, rafts are required for activation of the phosphoinositide 3-kinase/Akt signaling pathway in the early stage of infection (Das et al., 2010). For initiation of this cascade, recruitment of the receptor HSP70 into rafts is necessary (Zhu et al., 2012b). All these results suggest the possible involvement of rafts as a platform to concentrate JEV particles and their cellular receptors, and the subsequent virus internalization by CCPs.

On the other hand, there is emerging evidence that some viruses take advantage of cross-talk between clathrin- and caveolae-mediated pathways. Specifically, there are studies that propose that JC virus (JCV) (Querbes et al., 2006), bovine papillomavirus type 1 (BPV1) (Laniosz et al., 2008) and human papillomavirus type 16 (HPV-16) (Laniosz et al., 2009) enter cells via CCPs which then require Cav1-mediated trafficking for infection.

Membrane Rafts in Viral Entry by Fusion

Fusion process is a crucial entry mechanism for certain enveloped viruses. Viral fusion proteins are stimulated by a signal during the attachment at the target cell -such as receptor or co-receptor binding or proton binding in an endosome- promoting a series of conformational changes. The hydrophobic segment known as “fusion peptide” triggers the viral-cell membrane fusion, process which requires cooperation between lipids and proteins (Rawat et al., 2003; Harrison, 2015; Barrett and Dutch, 2020). Viral proteins play an essential role in directing and catalyzing the process, but successful outcome may depend on the lipid composition of both viral and cell membranes. Although the role of rafts in viral entry by fusion has been examined recently (Risselada, 2017), the mechanisms by which dynamic raft components control the process remain unclear.

Several studies have shown the importance of cholesterol for viral fusion processes, not only in the cell plasma membrane (or endosomal membrane), but also within the viral envelope. This is the case of HIV-1 (Mañes et al., 2000; Guyader et al., 2002), ecotropic murine leukemia virus (E-MLV) (Lu et al., 2002), VZV (Hambleton et al., 2007), human parainfluenza virus type 3 (HPIV3) (Tang et al., 2019) and caprine parainfluenza virus type3 (CPIV3) (Li et al., 2020b). There are also studies that have implicated sphingolipids in fusion events. In HIV-1 infection (Table 1), glycosphingolipids such as globotriaosyl ceramide (Gb3) (Puri et al., 1998), GM3 ganglioside and galactosylceramide (GalCer) (Hammache et al., 1998a, b) interact with viral glycoproteins and are suggested to act as fusion cofactors, promoting receptor recruitment and clustering (Sáez-Cirión et al., 2002; Rawat et al., 2004, 2006; Viard et al., 2004). In SFV infection, SM has also been proposed to act as a fusion cofactor, possibly activating the viral fusion protein in a specific manner (Nieva et al., 1994).

The relevance of cholesterol and SM is not surprising, considering that several viral families, including Orthomyxoviridae, Paramyxoviridae, Filoviridae, and Retroviridae (Manié et al., 2000; Suomalainen, 2002) use membrane rafts as a budding site to egress the cell and, therefore, their viral envelopes are cholesterol- and sphingolipid- enriched. Lipid rafts and viral envelopes are not only similar in their lipid composition, but also in their protein composition. In both structures, transmembrane proteins are enriched in palmitoylated and phosphorylated residues, although the TMDs of viral proteins are generally shorter and have a smaller accessible surface area per residue. Viral envelope proteins differ from raft proteins in other posttranslational modifications. For example, viral proteins have shorter myristoyl residues and higher phosphorylation in terms of protein fraction (Yurtsever and Lorent, 2020). Budding site selection is a fundamental step in the life cycle of enveloped viruses, as it determines the lipid and protein composition of their envelopes, which will influence the future stability and infectivity of virions. In HIV-1 infections (Table 1), the virulence factor Nef promotes HIV-1 budding from lipid rafts (Figure 4). Consequently, in the presence of Nef, viral envelopes contain more ganglioside GM1 -a major component of rafts- and infectivity of HIV-1 is significantly increased (Zheng et al., 2001; Mukhamedova et al., 2019).

FIGURE 4
www.frontiersin.org

Figure 4. Human immunodeficiency virus type 1 (HIV-1) entry by raft-dependent fusion in T lymphocytes. HIV-1 binds to CD4 receptor and CCR5 co-receptor within rafts. Interaction of CD4 with gp120 induces conformation changes which recruit the CXCR4 co-receptor to the periphery of the raft. Fusogenic activity of gp41 promotes fusion of the viral envelope and plasma membrane. The HIV-1 virulence factor Nef promotes budding of HIV-1 from membrane rafts, generating a viral progeny with a higher proportion of rafts in their envelopes and, thus, a higher infectivity. Viral entry by fusion in a raft-dependent manner has been also observed in EBOV, HSV-1, and MHV infections.

To demonstrate the implication of rafts in the fusion process, some studies have analyzed the association of viral fusion proteins with cell membrane rafts, with the GP2 viral fusion protein of EBOV being one example (Freitas et al., 2011) (Table 1). Other studies have proven the presence of receptors necessary for fusion within lipid rafts, as is the case of HIV-1 infection (Table 1 and Figure 4). CD4 receptor (Millán et al., 1999; Kozak et al., 2002) and CCR5 co-receptor of HIV-1 (Popik et al., 2002) are located in raft domains of T lymphocytes. The CXCR4 co-receptor is almost absent on rafts, but the interaction of CD4 with viral glycoprotein gp120 induces conformational changes which recruit CXCR4 to the periphery of the raft (Popik et al., 2002; Kamiyama et al., 2009). Cholesterol has been proposed to organize this multimeric envelope/receptor complex in clusters (Sáez-Cirión et al., 2002; Viard et al., 2002). However, it has been also suggested that the presence of HIV-1 receptors in rafts is not required for fusion process, and that cholesterol modulates the HIV-1 entry independently of its ability to promote raft formation (Percherancier et al., 2003). On the other hand, recent studies have demonstrated that the interfaces between Lo and Ld lipid domains are the predominant sites of HIV-1 fusion. Moreover, the presence of Lo domains in the cell membrane increases the overall fusion kinetics (Yang et al., 2016, 2017). The raft boundary can act as an attractor for viral fusion peptides (Molotkovsky et al., 2018).

The involvement of rafts in fusion process may be very elusive, not only in HIV-1 infection, but also in other viruses. In murine hepatitis virus (MHV) infections, cholesterol is necessary for fusion, even being proposed as an essential fusion membrane cofactor (Thorp and Gallagher, 2004), and the viral spike (fusion) protein is associated with rafts on the plasma membrane (Choi et al., 2005). However, the fusion receptor CEACAM is not located in rafts (Thorp and Gallagher, 2004), MHV does not incorporate rafts into the virion and the attachment step takes places in non-raft regions. It has been proposed that although MHV binding occurs in non-raft regions, the virus shifts to membrane rafts for viral entry (Choi et al., 2005).

Choice of Viral Raft-Mediated Entry Pathways

Since viruses can exploit more than one entry mechanism, even a combination of raft-dependent and -independent processes is possible. One such example is influenza A virus (IAV) (Table 1), whose main entry mechanism is via clathrin (Lakadamyali et al., 2008), but can also enter by non-clathrin non-caveolae endocytosis (Sieczkarski and Whittaker, 2002; Rust et al., 2004) and macropinocytosis (de Vries et al., 2011). Recently it has been proposed that rafts may play a role in IAV entry, acting as host attachment factors for multivalent binding, possibly through a raft-dependent endocytic pathway (Verma et al., 2018). Another example is EBOV (Table 1), which enters cells mostly by macropinocytosis (Nanbo et al., 2010; Saeed et al., 2010) but also by clathrin- (Bhattacharyya et al., 2010; Aleksandrowicz et al., 2011) and caveolae-mediated endocytosis (Bavari et al., 2002; Empig and Goldsmith, 2002; Sanchez, 2007).

Generally, a specific viral entry pathway prevails over the others. The choice of primary entry mechanism can sometimes be virus strain-specific. For instance, in keratinocytes, human papillomavirus (HPV) type 31 (Table 2) enters via caveolae (Smith et al., 2007), whereas HPV type 16 enters via clathrin (Laniosz et al., 2009). Another example is West Nile virus (WNV) in Vero cells (Table 1); the Sarafend strain binds to αvβ3 integrin and enters by CME (Chu and Ng, 2004a, b), whereas the NY385-99 strain exploits a raft-dependent endocytic route which is not associated with the integrin (Medigeshi et al., 2008). On the other hand, the choice of primary entry mechanism may often depend on the cell type. Though JEV (Table 1) uses a caveolae-mediated endocytosis in neurons (Zhu et al., 2012a; Kalia et al., 2013), it changes to a CME in Vero and Huh7 cells (Nawa et al., 2003; Tani et al., 2010), neural stem cells (Das et al., 2010) and porcine kidney cells (Yang et al., 2013).

In general, the cellular determinants of the route of viral entry are unknown. However, there are studies that have proposed certain cellular factors that determine this choice. For instance, αVβ3-integrin on the cell surface determines which entry pathway is used by HSV-1 (Table 1); in its presence, HSV-1 entry is mediated by cholesterol and dynamin-II, whereas in cells lacking the integrin, HSV-1 entry is independent of both. Also, integrin overexpression may favor the HSV-1 entry by macropinocytosis in certain cells (Gianni et al., 2010; Gianni and Campadelli-Fiume, 2012). The same authors have studied the interaction between different integrins and the HSV-1 glycoproteins gH/gL. Whereas αvβ8-integrin promoted viral endocytosis mediated by cholesterol and dynamin-II, αvβ6-integrin favored an endocytic mechanism independent of both (Gianni et al., 2013). Another example is EV71 infection (Table 2). The human scavenger receptor class B member 2 (hSCARB2) activates CME (Lin et al., 2012), whereas the receptor P-selectin glycoprotein ligand-1 (PSGL-1) initiates EV71 endocytosis mediated by caveolae (Lin et al., 2013).

Conclusion

The role of membrane rafts in viral entry is not easy to elucidate. Viral entry is a complex process composed of different steps, and raft domains may not be implicated in all of them. In some cases, raft participation has only been demonstrated during the attachment event, without insight about an involvement in endocytosis or fusion. In other cases, there is a clear dependence between rafts and viral endocytosis, but initial virus binding occurs in non-raft domains. These studies become more complicated due to the fact that one virus can hijack multiple entry mechanisms in the same cell. Moreover, entry pathways can change according to viral strain and cell line, and the reasons for the predominance of one entry pathway over the others are not clear most of the time. Finally, it is important to consider that although many studies analyze the effects of cholesterol depletion in viral entry, cholesterol dependence may not necessarily imply the participation of membrane rafts, but rather need more research to draw conclusions. The same precaution should be taken when extrapolating data obtained from DRMs, as well as when using certain entry controls, such as SV40 or cholera toxin B, which have been shown to exploit different endocytic pathways.

Although many questions remain to be answered, current studies have already shown the relevance of membrane rafts as portals for viral entry. Several viruses that hijack membrane rafts to enter the cells are pathogens of public health importance. Knowledge about the entry mechanisms mediated by rafts can help us to understand their life cycles and, as a consequence, may drive forward future discoveries of novel antiviral therapies.

Author Contributions

IR and RB-M: conceptualization. IR: writing—original draft preparation. IR, RB-M, SA, and JL-G: writing—review and editing. JL-G: project administration. RB-M and JL-G: funding acquisition. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Ministerio de Ciencia e Innovación, Spain. Grant Number PID2019 110570GB-I00.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The professional editing service NB Revisions was used for technical preparation of the text prior to submission.

References

Ahn, A., Gibbons, D. L., and Kielian, M. (2002). The fusion peptide of Semliki forest virus associates with sterol-rich membrane domains. J. Virol. 76, 3267–3275. doi: 10.1128/jvi.76.7.3267-3275.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Aleksandrowicz, P., Marzi, A., Biedenkopf, N., Beimforde, N., Becker, S., Hoenen, T., et al. (2011). Ebola virus enters host cells by macropinocytosis and clathrin-mediated endocytosis. J. Infect. Dis. 204, S957–S967.

Google Scholar

Anderson, H. A., Chen, Y., and Norkin, L. C. (1996). Bound simian virus 40 translocates to caveolin-enriched membrane domains, and its entry is inhibited by drugs that selectively disrupt caveolae. Mol. Biol. Cell 7, 1825–1834. doi: 10.1091/mbc.7.11.1825

PubMed Abstract | CrossRef Full Text | Google Scholar

Anderson, H. A., Chen, Y., and Norkin, L. C. (1998). MHC class I molecules are enriched in caveolae but do not enter with simian virus 40. J. Gen. Virol. 79, 1469–1477.

Google Scholar

Arias, C. F., Isa, P., Guerrero, C. A., Méndez, E., Zárate, S., López, T., et al. (2002). Molecular biology of rotavirus cell entry. Arch. Med. Res. 33, 356–361. doi: 10.1016/S0188-4409(02)00374-0

CrossRef Full Text | Google Scholar

Audi, A., Soudani, N., Dbaibo, G., and Zaraket, H. (2020). Depletion of host and viral sphingomyelin impairs influenza virus infection. Front. Microbiol. 11:612. doi: 10.3389/fmicb.2020.00612

CrossRef Full Text | Google Scholar

Bacsó, Z., Bene, L., Damjanovich, L., and Damjanovich, S. (2002). INF-γ rearranges membrane topography of MHC-I and ICAM-1 in colon carcinoma cells. Biochem. Biophys. Res. Commun. 290, 635–640. doi: 10.1006/bbrc.2001.6246

PubMed Abstract | CrossRef Full Text | Google Scholar

Baglivo, M., Baronio, M., Natalini, G., Beccari, T., Chiurazzi, P., Fulcheri, E., et al. (2020). Natural small molecules as inhibitors of coronavirus lipid-dependent attachment to host cells: a possible strategy for reducing SARS-COV-2 infectivity? Acta Biomed. 91, 161–164. doi: 10.23750/abm.v91i1.9402

PubMed Abstract | CrossRef Full Text | Google Scholar

Barnes, K., Ingram, J. C., Bennett, M. D. M., Stewart, G. W., and Baldwin, S. A. (2004). Methyl-β-cyclodextrin stimulates glucose uptake in Clone 9 cells: a possible role for lipid rafts. Biochem. J. 378, 343–351. doi: 10.1042/BJ20031186

PubMed Abstract | CrossRef Full Text | Google Scholar

Barrett, C. T., and Dutch, R. E. (2020). Viral membrane fusion and the transmembrane domain. Viruses 12, 1–27. doi: 10.3390/v12070693

PubMed Abstract | CrossRef Full Text | Google Scholar

Barrow, E., Nicola, A. V., and Liu, J. (2013). Multiscale perspectives of virus entry via endocytosis. Virol. J. 10:177.

Google Scholar

Bastiani, M., Liu, L., Hill, M. M., Jedrychowski, M. P., Nixon, S. J., Lo, H. P., et al. (2009). MURC/Cavin-4 and cavin family members form tissue-specific caveolar complexes. J. Cell Biol. 185, 1259–1273. doi: 10.1083/jcb.200903053

PubMed Abstract | CrossRef Full Text | Google Scholar

Bastiani, M., and Parton, R. G. (2010). Caveolae at a glance. J. Cell Sci. 123, 3831–3836. doi: 10.1242/jcs.070102

PubMed Abstract | CrossRef Full Text | Google Scholar

Basturea, G. (2019). Endocytosis. Mater. Methods 9:2752. doi: 10.13070/mm.en.9.2752

CrossRef Full Text | Google Scholar

Bavari, S., Bosio, C. M., Wiegand, E., Ruthel, G., Will, A. B., Geisbert, T. W., et al. (2002). Lipid raft microdomains: a gateway for compartmentalized trafficking of Ebola and Marburg viruses. J. Exp. Med. 195, 593–602. doi: 10.1084/jem.20011500

PubMed Abstract | CrossRef Full Text | Google Scholar

Beer, C., Andersen, D. S., Rojek, A., and Pedersen, L. (2005). Caveola-dependent endocytic entry of amphotropic murine Leukemia Virus. J. Virol. 79, 10776–10787. doi: 10.1128/jvi.79.16.10776-10787.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Beer, C., and Pedersen, L. (2006). Amphotropic murine leukemia virus is preferentially attached to cholesterol-rich microdomains after binding to mouse fibroblasts. Virol. J. 3:21.

Google Scholar

Bender, F. C., Whitbeck, J. C., Ponce de Leon, M., Lou, H., Eisenberg, R. J., and Cohen, G. H. (2003). Specific association of glycoprotein B with lipid rafts during herpes simplex virus entry. J. Virol. 77, 9542–9552. doi: 10.1128/jvi.77.17.9542-9552.2003

PubMed Abstract | CrossRef Full Text | Google Scholar

Bhattacharyya, S., Warfield, K. L., Ruthel, G., Bavari, S., Aman, M. J., and Hope, T. J. (2010). Ebola virus uses clathrin-mediated endocytosis as an entry pathway. Virology 401, 18–28. doi: 10.1016/j.virol.2010.02.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Bhella, D. (2015). The role of cellular adhesion molecules in virus attachment and entry. Philos. Trans. R. Soc. B Biol. Sci. 370:20140035. doi: 10.1098/rstb.2014.0035

PubMed Abstract | CrossRef Full Text | Google Scholar

Bieberich, E. (2018). Sphingolipids and lipid rafts: novel concepts and methods of analysis. Chem. Phys. Lipids 216, 114–131. doi: 10.1016/j.chemphyslip.2018.08.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Blanchard, E., Belouzard, S., Goueslain, L., Wakita, T., Dubuisson, J., Wychowski, C., et al. (2006). Hepatitis C Virus entry depends on clathrin-mediated endocytosis. J. Virol. 80, 6964–6972.

Google Scholar

Bousarghin, L., Touzé, A., Sizaret, P.-Y., and Coursaget, P. (2003). Human Papillomavirus types 16, 31, and 58 use different endocytosis pathways to enter cells. J. Virol. 77, 3846–3850. doi: 10.1128/jvi.77.6.3846-3850.2003

PubMed Abstract | CrossRef Full Text | Google Scholar

Briand, N., Dugail, I., and Le Lay, S. (2011). Cavin proteins: new players in the caveolae field. Biochimie 93, 71–77. doi: 10.1016/j.biochi.2010.03.022

PubMed Abstract | CrossRef Full Text | Google Scholar

Brown, D. A. (2006). Lipid rafts, detergent-resistant membranes, and raft targeting signals. Physiology 21, 430–439. doi: 10.1152/physiol.00032.2006

PubMed Abstract | CrossRef Full Text | Google Scholar

Bukrinsky, M. I., Mukhamedova, N., and Sviridov, D. (2020). Lipid rafts and pathogens: the art of deception and exploitation. J. Lipid Res. 61, 601–610. doi: 10.1194/jlr.TR119000391

PubMed Abstract | CrossRef Full Text | Google Scholar

Cambi, A., De Lange, F., Van Maarseveen, N. M., Nijhuis, M., Joosten, B., Van Dijk, E. M. H. P., et al. (2004). Microdomains of the C-type lectin DC-SIGN are portals for virus entry into dendritic cells. J. Cell Biol. 164, 145–155. doi: 10.1083/jcb.200306112

PubMed Abstract | CrossRef Full Text | Google Scholar

Cantín, C., Holguera, J., Ferrerira, L., Villar, E., and Muñoz-Barroso, I. (2007). Newcastle disease virus may enter cells by caveolae-mediated endocytosis. J. Gen. Virol. 88, 559–569.

Google Scholar

Carter, G. C., Bernstone, L., Baskaran, D., and James, W. (2011). HIV-1 infects macrophages by exploiting an endocytic route dependent on dynamin, Rac1 and Pak1. Virology 409, 234–250. doi: 10.1016/j.virol.2010.10.018

PubMed Abstract | CrossRef Full Text | Google Scholar

Carter, G. C., Bernstone, L., Sangani, D., Bee, J. W., Harder, T., and James, W. (2009). HIV entry in macrophages is dependent on intact lipid rafts. Virology 386, 192–202. doi: 10.1016/j.virol.2008.12.031

PubMed Abstract | CrossRef Full Text | Google Scholar

Chakraborty, S., ValiyaVeettil, M., Sadagopan, S., Paudel, N., and Chandran, B. (2011). c-Cbl-mediated selective virus-receptor translocations into lipid rafts regulate productive Kaposi’s sarcoma-associated Herpesvirus infection in endothelial cells. J. Virol. 85, 12410–12430.

Google Scholar

Chakraborty, S., Veettil, M. V., Bottero, V., and Chandran, B. (2012). Kaposi’s sarcoma-associated herpesvirus interacts with EphrinA2 receptor to amplify signaling essential for productive infection. Proc. Natl. Acad. Sci. U.S.A. 109, 7146–7147. doi: 10.1073/pnas.1119592109

PubMed Abstract | CrossRef Full Text | Google Scholar

Chamberlain, L. H. (2004). Detergents as tools for the purification and classification of lipid rafts. FEBS Lett. 559, 1–5. doi: 10.1016/S0014-5793(04)00050-X

CrossRef Full Text | Google Scholar

Chazal, N., and Gerlier, D. (2003). Virus entry, assembly, budding, and membrane rafts. Microbiol. Mol. Biol. Rev. 67, 226–237. doi: 10.1128/mmbr.67.2.226-237.2003

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, S., He, H., Yang, H., Tan, B., Liu, E., Zhao, X., et al. (2019a). The role of lipid rafts in cell entry of human metapneumovirus. J. Med. Virol. 91, 949–957. doi: 10.1002/jmv.25414

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, Y. J., Liu, X., and Tsai, B. (2019b). SV40 hijacks cellular transport, membrane penetration, and disassembly machineries to promote infection. Viruses 11:917. doi: 10.3390/v11100917

PubMed Abstract | CrossRef Full Text | Google Scholar

Cheng, J. P. X., and Nichols, B. J. (2016). Caveolae: One function or many? Trends Cell Biol. 26, 177–189. doi: 10.1016/j.tcb.2015.10.010

PubMed Abstract | CrossRef Full Text | Google Scholar

Cherukuri, A., Shoham, T., Sohn, H. W., Levy, S., Brooks, S., Carter, R., et al. (2004). The tetraspanin CD81 is necessary for partitioning of coligated CD19/CD21-B cell antigen receptor complexes into signaling-active lipid rafts. J. Immunol. 172, 370–380. doi: 10.4049/jimmunol.172.1.370

PubMed Abstract | CrossRef Full Text | Google Scholar

Chinnapen, D. J. F., Chinnapen, H., Saslowsky, D., and Lencer, W. I. (2007). Rafting with cholera toxin: endocytosis and trafficking from plasma membrane to ER. FEMS Microbiol. Lett. 266, 129–137. doi: 10.1111/j.1574-6968.2006.00545.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Choi, K. S., Aizaki, H., and Lai, M. M. C. (2005). Murine Coronavirus requires lipid rafts for virus entry and cell-cell fusion but not for virus release. J. Virol. 79, 9862–9871. doi: 10.1128/jvi.79.15.9862-9871.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Chu, J. J.-H., and Ng, M. L. (2004a). Interaction of West Nile Virus with αvβ3 integrin mediates virus entry into cells. J. Biol. Chem. 279, 54533–54541. doi: 10.1074/jbc.M410208200

PubMed Abstract | CrossRef Full Text | Google Scholar

Chu, J. J. H., and Ng, M. L. (2004b). Infectious entry of west nile virus occurs through a clathrin-mediated endocytic pathway. J. Virol. 78, 10543–10555. doi: 10.1128/jvi.78.19.10543-10555.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

Chung, C.-S., Huang, C.-Y., and Chang, W. (2005). Vaccinia virus penetration requires cholesterol and results in specific viral envelope proteins associated with lipid rafts. J. Virol. 79, 1623–1634. doi: 10.1128/jvi.79.3.1623-1634.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Colin, M., Mailly, L., Rogée, S., and D’Halluin, J. C. (2005). Efficient Species C HAdV infectivity in plasmocytic cell lines using a clathrin-independent lipid Raft/Caveola Endocytic Route. Mol. Ther. 11, 224–236. doi: 10.1016/j.ymthe.2004.10.007

PubMed Abstract | CrossRef Full Text | Google Scholar

Cossart, P., and Helenius, A. (2014). Endocytosis of Viruses and Bacteria. Cold Spring Harb. Perspect. Biol. 6:a016972. doi: 10.1101/cshperspect.a016972

PubMed Abstract | CrossRef Full Text | Google Scholar

Coyne, C. B., and Bergelson, J. M. (2006). Virus-Induced Abl and Fyn Kinase signals permit coxsackievirus entry through epithelial tight junctions. Cell 124, 119–131. doi: 10.1016/j.cell.2005.10.035

PubMed Abstract | CrossRef Full Text | Google Scholar

Damm, E. M., Pelkmans, L., Kartenbeck, J., Mezzacasa, A., Kurzchalia, T., and Helenius, A. (2005). Clathrin- and caveolin-1-independent endocytosis: entry of simian virus 40 into cells devoid of caveolae. J. Cell Biol. 168, 477–488. doi: 10.1083/jcb.200407113

PubMed Abstract | CrossRef Full Text | Google Scholar

Das, M., and Das, D. (2009). Lipid raft in cardiac health and disease. Curr. Cardiol. Rev. 5, 105–111. doi: 10.2174/157340309788166660

PubMed Abstract | CrossRef Full Text | Google Scholar

Das, S., Chakraborty, S., and Basu, A. (2010). Critical role of lipid rafts in virus entry and activation of phosphoinositide 3’ kinase/Akt signaling during early stages of Japanese encephalitis virus infection in neural stem/progenitor cells. J. Neurochem. 115, 537–549. doi: 10.1111/j.1471-4159.2010.06951.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Day, C. A., and Kenworthy, A. K. (2015). Functions of cholera toxin B-subunit as a raft cross-linker. Essays Biochem. 57, 135–145. doi: 10.1042/BSE0570135

PubMed Abstract | CrossRef Full Text | Google Scholar

de Vries, E., Tscherne, D. M., Wienholts, M. J., Cobos-Jiménez, V., Scholte, F., García-Sastre, A., et al. (2011). Dissection of the influenza A Virus endocytic routes reveals macropinocytosis as an alternative entry pathway. PLoS Pathog. 7:e1001329. doi: 10.1371/journal.ppat.1001329

PubMed Abstract | CrossRef Full Text | Google Scholar

Desplanques, A. S., Nauwynck, H. J., Vercauteren, D., Geens, T., and Favoreel, H. W. (2008). Plasma membrane cholesterol is required for efficient pseudorabies virus entry. Virology 376, 339–345. doi: 10.1016/j.virol.2008.03.039

PubMed Abstract | CrossRef Full Text | Google Scholar

Donaldson, J. G. (2019). Macropinosome formation, maturation and membrane recycling: lessons from clathrin-independent endosomal membrane systems. Philos. Trans. R. Soc. B Biol. Sci. 374:20180148. doi: 10.1098/rstb.2018.0148

PubMed Abstract | CrossRef Full Text | Google Scholar

Dou, X., Li, Y., Han, J., Zarlenga, D. S., Zhu, W., Ren, X., et al. (2018). Cholesterol of lipid rafts is a key determinant for entry and post-entry control of porcine rotavirus infection. BMC Vet. Res. 14:45. doi: 10.1186/s12917-018-1366-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Dugan, A. S., Eash, S., and Atwood, W. J. (2006). Update on BK virus entry and intracellular trafficking. Transpl. Infect. Dis. 8, 62–67. doi: 10.1111/j.1399-3062.2006.00153.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Eash, S., and Atwood, W. J. (2005). Involvement of cytoskeletal components in BK Virus infectious entry. J. Virol. 79, 11734–11741. doi: 10.1128/jvi.79.18.11734-11741.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Eash, S., Querbes, W., and Atwood, W. J. (2004). Infection of vero cells by BK virus is dependent on caveolae. J. Virol. 78, 11583–11590. doi: 10.1128/jvi.78.21.11583-11590.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

El-Sayed, A., and Harashima, H. (2013). Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis. Mol. Ther. 21, 1118–1130. doi: 10.1038/mt.2013.54

PubMed Abstract | CrossRef Full Text | Google Scholar

Empig, C. J., and Goldsmith, M. A. (2002). Association of the caveola vesicular system with cellular entry by filoviruses. J. Virol. 76, 5266–5270. doi: 10.1128/jvi.76.10.5266-5270.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Evans, M. J., Von Hahn, T., Tscherne, D. M., Syder, A. J., Panis, M., Wölk, B., et al. (2007). Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry. Nature 446, 801–805. doi: 10.1038/nature05654

PubMed Abstract | CrossRef Full Text | Google Scholar

Freitas, M. S., Follmer, C., Costa, L. T., Vilani, C., Lucia Bianconi, M., Achete, C. A., et al. (2011). Measuring the strength of interaction between the Ebola fusion peptide and lipid rafts: implications for membrane fusion and virus infection. PLoS One 6:e15756. doi: 10.1371/journal.pone.0015756

PubMed Abstract | CrossRef Full Text | Google Scholar

Ghez, D., Lepelletier, Y., Lambert, S., Fourneau, J.-M., Blot, V., Janvier, S., et al. (2006). Neuropilin-1 is involved in human T-Cell Lymphotropic virus type 1 entry. J. Virol. 80, 6844–6854.

Google Scholar

Gianni, T., and Campadelli-Fiume, G. (2012). αvβ3-integrin relocalizes nectin1 and routes herpes simplex virus to lipid rafts. J. Virol. 86, 2850–2855.

Google Scholar

Gianni, T., Gatta, V., and Campadelli-Fiume, G. (2010). αvβ3-integrin routes herpes simplex virus to an entry pathway dependent on cholesterol-rich lipid rafts and dynamin2. Proc. Natl. Acad. Sci. U.S.A. 107, 22260–22265. doi: 10.1073/pnas.1014923108

PubMed Abstract | CrossRef Full Text | Google Scholar

Gianni, T., Salvioli, S., Chesnokova, L. S., Hutt-Fletcher, L. M., and Campadelli-Fiume, G. (2013). αvβ6- and αvβ8-integrins serve as interchangeable receptors for HSV gH/gL to promote endocytosis and activation of membrane fusion. PLoS Pathog. 9:e1003806. doi: 10.1371/journal.ppat.1003806

PubMed Abstract | CrossRef Full Text | Google Scholar

Gilbert, J., and Benjamin, T. (2004). Uptake pathway of polyomavirus via ganglioside GD1a. J. Virol. 78, 12259–12267. doi: 10.1128/jvi.78.22.12259-12267.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

Glende, J., Schwegmann-Wessels, C., Al-Falah, M., Pfefferle, S., Qu, X., Deng, H., et al. (2008). Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2. Virology 381, 215–221. doi: 10.1016/j.virol.2008.08.026

PubMed Abstract | CrossRef Full Text | Google Scholar

Gobeil, L.-A., Lodge, R., and Tremblay, M. J. (2013). Macropinocytosis-like HIV-1 internalization in macrophages is CCR5 dependent and leads to efficient but delayed degradation in endosomal compartments. J. Virol. 87, 735–745.

Google Scholar

Goodman, A. G., Martín-Acebes, M. A., Sviridov, D., Maria del, Ángel, R., Fidel Osuna-Ramos, J., et al. (2018). The role of host cholesterol during Flavivirus infection. Front. Cell. Infect. Microbiol. 8:388. doi: 10.3389/fcimb.2018.00388

PubMed Abstract | CrossRef Full Text | Google Scholar

Graham, J. M. (2002). Purification of lipid rafts from cultured cells. ScientificWorldJournal. 2, 1662–1666. doi: 10.1100/tsw.2002.846

PubMed Abstract | CrossRef Full Text | Google Scholar

Greenlee, J. D., Subramanian, T., Liu, K., and King, M. R. (2020). Rafting down the metastatic cascade: the role of lipid rafts in cancer metastasis, cell death, and clinical outcomes. Cancer Res. doi: 10.1158/0008-5472.CAN-20-2199 [Epub ahead of print].

CrossRef Full Text | PubMed Abstract | Google Scholar

Greve, J. M., Davis, G., Meyer, A. M., Forte, C. P., Yost, S. C., Marlor, C. W., et al. (1989). The major human rhinovirus receptor is ICAM-1. Cell 56, 839–847.

Google Scholar

Grove, J., and Marsh, M. (2011). The cell biology of receptor-mediated virus entry. J. Cell Biol. 195, 1071–1082. doi: 10.1083/jcb.201108131

PubMed Abstract | CrossRef Full Text | Google Scholar

Guerrero, C. A., and Moreno, L. P. (2012). Rotavirus receptor proteins Hsc70 and integrin avβ3 are located in the lipid microdomains of animal intestinal cells. Acta Virol. 56, 63–70. doi: 10.4149/av_2012_01_63

CrossRef Full Text | Google Scholar

Guirland, C., Suzuki, S., Kojima, M., Lu, B., and Zheng, J. Q. (2004). Lipid rafts mediate chemotropic guidance of nerve growth cones. Neuron 42, 51–62.

Google Scholar

Guo, C.-J., Liu, D., Wu, Y.-Y., Yang, X.-B., Yang, L.-S., Mi, S., et al. (2011). Entry of Tiger Frog Virus (an Iridovirus) into HepG2 Cells via a pH-dependent, atypical, caveola-mediated endocytosis pathway. J. Virol. 85, 6416–6426.

Google Scholar

Guo, C.-J., Wu, Y.-Y., Yang, L.-S., Yang, X.-B., He, J., Mi, S., et al. (2012). Infectious spleen and kidney Necrosis Virus (a Fish Iridovirus) Enters Mandarin Fish Fry Cells via Caveola-Dependent Endocytosis. J. Virol. 86, 2621–2631.

Google Scholar

Guo, H., Huang, M., Yuan, Q., Wei, Y., Gao, Y., Mao, L., et al. (2017). The important role of lipid raft-mediated attachment in the infection of cultured cells by coronavirus infectious bronchitis virus beaudette strain. PLoS One 12:e0170123. doi: 10.1371/journal.pone.0170123

PubMed Abstract | CrossRef Full Text | Google Scholar

Guyader, M., Kiyokawa, E., Abrami, L., Turelli, P., and Trono, D. (2002). Role for human immunodeficiency virus type 1 membrane cholesterol in viral internalization. J. Virol. 76, 10356–10364. doi: 10.1128/jvi.76.20.10356-10364.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Hahn, A. S., Kaufmann, J. K., Wies, E., Naschberger, E., Panteleev-Ivlev, J., Schmidt, K., et al. (2012). The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi’s sarcoma-associated herpesvirus. Nat. Med. 18, 961–966. doi: 10.1038/nm.2805

PubMed Abstract | CrossRef Full Text | Google Scholar

Hambleton, S., Steinberg, S. P., Gershon, M. D., and Gershon, A. A. (2007). Cholesterol dependence of varicella-zoster virion entry into target cells. J. Virol. 81, 7548–7558.

Google Scholar

Hammache, D., Piéroni, G., Yahi, N., Delézay, O., Koch, N., Lafont, H., et al. (1998a). Specific interaction of HIV-1 and HIV-2 surface envelope glycoproteins with monolayers of galactosylceramide and ganglioside GM3. J. Biol. Chem. 273, 7967–7971. doi: 10.1074/jbc.273.14.7967

PubMed Abstract | CrossRef Full Text | Google Scholar

Hammache, D., Yahi, N., Piéroni, G., Ariasi, F., Tamalet, C., and Fantini, J. (1998b). Sequential interaction of CD4 and HIV-1 gp120 with a reconstituted membrane patch of ganglioside GM3: implications for the role of glycolipids as potential HIV-1 fusion cofactors. Biochem. Biophys. Res. Commun. 246, 117–122. doi: 10.1006/bbrc.1998.8531

PubMed Abstract | CrossRef Full Text | Google Scholar

Harmon, B., Schudel, B. R., Maar, D., Kozina, C., Ikegami, T., Tseng, C.-T. K., et al. (2012). Rift valley fever virus strain MP-12 enters mammalian host cells via caveola-mediated endocytosis. J. Virol. 86, 12954–12970.

Google Scholar

Harrison, S. C. (2015). Viral membrane fusion. Virology 479-480, 498–507. doi: 10.1016/j.virol.2015.03.043

PubMed Abstract | CrossRef Full Text | Google Scholar

Hasebe, R., Sasaki, M., Sawa, H., Wada, R., Umemura, T., and Kimura, T. (2009). Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathways. Virology 393, 198–209. doi: 10.1016/j.virol.2009.07.032

PubMed Abstract | CrossRef Full Text | Google Scholar

Hayer, A., Stoeber, M., Ritz, D., Engel, S., Meyer, H. H., and Helenius, A. (2010a). Caveolin-1 is ubiquitinated and targeted to intralumenal vesicles in endolysosomes for degradation. J. Cell Biol. 191, 615–629. doi: 10.1083/jcb.201003086

PubMed Abstract | CrossRef Full Text | Google Scholar

Hayer, A., Stoeber, M., Ritz, D., Engel, S., Meyer, H. H., and Helenius, A. (2010b). Revisiting caveolin trafficking: the end of the caveosome. J. Cell Biol. 191, 615–629.

Google Scholar

Helle, F., and Dubuisson, J. (2008). Hepatitis C virus entry into host cells. Cell. Mol. Life Sci. 65, 100–112.

Google Scholar

Hoffmann, M., Kleine-Weber, H., Schroeder, S., Krüger, N., Herrler, T., Erichsen, S., et al. (2020). SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell 181, 271–280.e8. doi: 10.1016/j.cell.2020.02.052

PubMed Abstract | CrossRef Full Text | Google Scholar

Huang, C.-Y., Lu, T.-Y., Bair, C.-H., Chang, Y.-S., Jwo, J.-K., and Chang, W. (2008). A novel cellular protein, VPEF, facilitates vaccinia virus penetration into HeLa cells through fluid phase endocytosis. J. Virol. 82, 7988–7999.

Google Scholar

Huang, W. R., Wang, Y. C., Chi, P. I., Wang, L., Wang, C. Y., Lin, C. H., et al. (2011). Cell entry of avian reovirus follows a caveolin-1-mediated and dynamin-2-dependent endocytic pathway that requires activation of p38 Mitogen-activated Protein Kinase (MAPK) and Src Signaling Pathways as Well as Microtubules and Small GTPase Rab5 Protein. J. Biol. Chem. 286, 30780–30794. doi: 10.1074/jbc.M111.257154

PubMed Abstract | CrossRef Full Text | Google Scholar

Iša, P., Realpe, M., Romero, P., López, S., and Arias, C. F. (2004). Rotavirus RRV associates with lipid membrane microdomains during cell entry. Virology 322, 370–381. doi: 10.1016/j.virol.2004.02.018

PubMed Abstract | CrossRef Full Text | Google Scholar

Izmailyan, R., Hsao, J.-C., Chung, C.-S., Chen, C.-H., Hsu, P. W.-C., Liao, C.-L., et al. (2012). Integrin 1 Mediates Vaccinia Virus Entry through Activation of PI3K/Akt Signaling. J. Virol. 86, 6677–6687.

Google Scholar

Jiang, Y., Liu, S., Shen, S., Guo, H., Huang, H., and Wei, W. (2020). Methyl-β-cyclodextrin inhibits EV-D68 virus entry by perturbing the accumulation of virus particles and ICAM-5 in lipid rafts. Antiviral Res. 176:104752. doi: 10.1016/j.antiviral.2020.104752

PubMed Abstract | CrossRef Full Text | Google Scholar

Jin, C., Che, B., Guo, Z., Li, C., Liu, Y., Wu, W., et al. (2020). Single virus tracking of Ebola virus entry through lipid rafts in living host cells. Biosaf. Heal. 2, 25–31. doi: 10.1016/j.bsheal.2019.12.009

CrossRef Full Text | Google Scholar

Jolly, C. L., and Sattentau, Q. J. (2013). Attachment factors. Adv. Exp. Med. Biol. 790, 1–23. doi: 10.1007/978-1-4614-7651-1_1

CrossRef Full Text | Google Scholar

Kaksonen, M., and Roux, A. (2018). Mechanisms of clathrin-mediated endocytosis. Nat. Rev. Mol. Cell Biol. 19, 313–326. doi: 10.1038/nrm.2017.132

PubMed Abstract | CrossRef Full Text | Google Scholar

Kalia, M., Khasa, R., Sharma, M., Nain, M., and Vrati, S. (2013). Japanese encephalitis virus infects neuronal cells through a clathrin-independent endocytic mechanism. J. Virol. 87, 148–162.

Google Scholar

Kamiyama, H., Yoshii, H., Tanaka, Y., Sato, H., Yamamoto, N., and Kubo, Y. (2009). Raft localization of CXCR4 is primarily required for X4-tropic human immunodeficiency virus type 1 infection. Virology 386, 23–31. doi: 10.1016/j.virol.2008.12.033

PubMed Abstract | CrossRef Full Text | Google Scholar

Kapadia, S. B., Barth, H., Baumert, T., McKeating, J. A., and Chisari, F. V. (2007). Initiation of hepatitis C virus infection is dependent on cholesterol and cooperativity between CD81 and scavenger receptor B type I. J. Virol. 81, 374–383.

Google Scholar

Karnovsky, M. J., Kleinfeld, A. M., Hoover, R. L., and Klausner, R. D. (1982). The concept of lipid domains in membranes. J. Cell Biol. 94, 1–6. doi: 10.1083/jcb.94.1.1

PubMed Abstract | CrossRef Full Text | Google Scholar

Khater, I. M., Liu, Q., Chou, K. C., Hamarneh, G., and Nabi, I. R. (2019). Super-resolution modularity analysis shows polyhedral caveolin-1 oligomers combine to form scaffolds and caveolae. Sci. Rep. 9:9888. doi: 10.1038/s41598-019-46174-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Koutsoudakis, G., Herrmann, E., Kallis, S., Bartenschlager, R., and Pietschmann, T. (2007). The level of CD81 cell surface expression is a key determinant for productive entry of hepatitis C virus into host cells. J. Virol. 81, 588–598.

Google Scholar

Kovtun, O., Tillu, V. A., Ariotti, N., Parton, R. G., and Collins, B. M. (2015). Cavin family proteins and the assembly of caveolae. J. Cell Sci. 128, 1269–1278. doi: 10.1242/jcs.167866

PubMed Abstract | CrossRef Full Text | Google Scholar

Koyama-Honda, I., Fujiwara, T. K., Kasai, R. S., Suzuki, K. G. N., Kajikawa, E., Tsuboi, H., et al. (2020). High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases. J. Cell Biol. 219:e202006125. doi: 10.1083/JCB.202006125

PubMed Abstract | CrossRef Full Text | Google Scholar

Kozak, S. L., Heard, J. M., and Kabat, D. (2002). Segregation of CD4 and CXCR4 into distinct lipid microdomains in T lymphocytes suggests a mechanism for membrane destabilization by human immunodeficiency virus. J. Virol. 76, 1802–1815. doi: 10.1128/jvi.76.4.1802-1815.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Krieger, S. E., Kim, C., Zhang, L., Marjomaki, V., and Bergelson, J. M. (2013). Echovirus 1 entry into polarized Caco-2 cells depends on dynamin, cholesterol, and cellular factors associated with macropinocytosis. J. Virol. 87, 8884–8895.

Google Scholar

Kumar, A., Xiao, Y.-P., Laipis, P. J., Fletcher, B. S., and Frost, S. C. (2004). Glucose deprivation enhances targeting of GLUT1 to lipid rafts in 3T3-L1 adipocytes. Am. J. Physiol. Metab. 286, E568–E576. doi: 10.1152/ajpendo.00372.2003

PubMed Abstract | CrossRef Full Text | Google Scholar

Lakadamyali, M. J., Rust, M., and Zhuang, X. (2008). Endocytosis of influenza viruses. Microbes Infect. 6, 929–936. doi: 10.1038/jid.2014.371

PubMed Abstract | CrossRef Full Text | Google Scholar

Laniosz, V., Dabydeen, S. A., Havens, M. A., and Meneses, P. I. (2009). Human papillomavirus type 16 infection of human keratinocytes requires clathrin and caveolin-1 and is brefeldin A sensitive. J. Virol. 83, 8221–8232.

Google Scholar

Laniosz, V., Holthusen, K. A., and Meneses, P. I. (2008). Bovine Papillomavirus Type 1: from Clathrin to Caveolin. J. Virol. 82, 6288–6298.

Google Scholar

Lee, A. G., Birdsall, N. J. M., Metcalfe, J. C., Toon, P. A., and Warren, G. B. (1974). Clusters in lipid bilayers and the interpretation of thermal effects in biological membranes. Biochemistry 13, 3699–3705. doi: 10.1021/bi00715a013

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, C.-J., Lin, H.-R., Liao, C.-L., and Lin, Y.-L. (2008). Cholesterol effectively blocks entry of Flavivirus. J. Virol. 82, 6470–6480.

Google Scholar

Legler, D. F., Doucey, M.-A., Schneider, P., Chapatte, L., Bender, F. C., and Bron, C. (2005). Differential insertion of GPI-anchored GFPs into lipid rafts of live cells. FASEB J. 19, 73–75. doi: 10.1096/fj.03-1338fje

PubMed Abstract | CrossRef Full Text | Google Scholar

Levental, I., Levental, K. R., and Heberle, F. A. (2020). Lipid rafts: controversies resolved, mysteries remain. Trends Cell Biol. 30, 341–353. doi: 10.1016/j.tcb.2020.01.009

CrossRef Full Text | Google Scholar

Li, M., Yan, P., Liu, Z., Cai, D., Luo, Y., Wu, X., et al. (2020a). Muscovy duck reovirus enters susceptible cells via a caveolae-mediated endocytosis-like pathway. Virus Res. 276:197806. doi: 10.1016/j.virusres.2019.197806

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, W., Yang, L., Mao, L., Liu, M., Li, J., Zhang, W., et al. (2020b). Cholesterol-rich lipid rafts both in cellular and viral membrane are critical for caprine parainfluenza virus type3 entry and infection in host cells. Vet. Microbiol. 248:108794. doi: 10.1016/j.vetmic.2020.108794

PubMed Abstract | CrossRef Full Text | Google Scholar

Lichtenberg, D., Goñi, F. M., and Heerklotz, H. (2005). Detergent-resistant membranes should not be identified with membrane rafts. Trends Biochem. Sci. 30, 430–436. doi: 10.1016/j.tibs.2005.06.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Liebl, D., Difato, F., Horníková, L., Mannová, P., Štokrová, J., and Forstová, J. (2006). Mouse polyomavirus enters early endosomes, requires their acidic pH for productive infection, and meets transferrin cargo in Rab11-Positive endosomes. J. Virol. 80, 4610–4622. doi: 10.1128/jvi.80.9.4610-4622.2006

PubMed Abstract | CrossRef Full Text | Google Scholar

Lin, H.-Y., Yang, Y.-T., Yu, S.-L., Hsiao, K.-N., Liu, C.-C., Sia, C., et al. (2013). Caveolar endocytosis is required for human PSGL-1-mediated enterovirus 71 infection. J. Virol. 87, 9064–9076.

Google Scholar

Lin, Y.-W., Lin, H.-Y., Tsou, Y.-L., Chitra, E., Hsiao, K.-N., Shao, H.-Y., et al. (2012). Human SCARB2-mediated entry and endocytosis of EV71. PLoS One 7:e30507. doi: 10.1371/journal.pone.0030507

PubMed Abstract | CrossRef Full Text | Google Scholar

Liu, N. Q., Lossinsky, A. S., Popik, W., Li, X., Gujuluva, C., Kriederman, B., et al. (2002). Human immunodeficiency virus type 1 enters brain microvascular endothelia by macropinocytosis dependent on lipid rafts and the mitogen-activated protein kinase signaling pathway. J. Virol. 76, 6689–6700. doi: 10.1128/jvi.76.13.6689-6700.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Lopez, S., and Arias, C. F. (2006). Early steps in rotavirus cell entry. Curr. Top. Microbiol. Immunol. 309, 39–66. doi: 10.1007/3-540-30773-7_2

CrossRef Full Text | Google Scholar

Lorent, J. H., Diaz-Rohrer, B., Lin, X., Spring, K., Gorfe, A. A., Levental, K. R., et al. (2017). Structural determinants and functional consequences of protein affinity for membrane rafts. Nat. Commun. 8:1219.

Google Scholar

Lu, X., and Silver, J. (2000). Ecotropic murine leukemia virus receptor is physically associated with caveolin and membrane rafts. Virology 276, 251–258. doi: 10.1006/viro.2000.0555

PubMed Abstract | CrossRef Full Text | Google Scholar

Lu, X., Xiong, Y., and Silver, J. (2002). Asymmetric requirement for cholesterol in receptor-bearing but not envelope-bearing membranes for fusion mediated by ecotropic murine leukemia virus. J. Virol. 76, 6701–6709. doi: 10.1128/jvi.76.13.6701-6709.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Lu, Y., Liu, D. X., and Tam, J. P. (2008). Lipid rafts are involved in SARS-CoV entry into Vero E6 cells. Biochem. Biophys. Res. Commun. 369, 344–349. doi: 10.1016/j.bbrc.2008.02.023

PubMed Abstract | CrossRef Full Text | Google Scholar

Lynch, R. D., Francis, S. A., McCarthy, K. M., Casas, E., Thiele, C., and Schneeberger, E. E. (2007). Cholesterol depletion alters detergent-specific solubility profiles of selected tight junction proteins and the phosphorylation of occludin. Exp. Cell Res. 313, 2597–2610. doi: 10.1016/j.yexcr.2007.05.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Mañes, S., del Real, G., Lacalle, R. A., Lucas, P., Gómez-Moutón, C., Sánchez-Palomino, S., et al. (2000). Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection. EMBO Rep. 1, 190–196. doi: 10.1093/EMBO-REPORTS/KVD025

PubMed Abstract | CrossRef Full Text | Google Scholar

Manié, S. N., Debreyne, S., Vincent, S., and Gerlier, D. (2000). Measles virus structural components are enriched into lipid raft microdomains: a potential cellular location for virus assembly. J. Virol. 74, 305–311. doi: 10.1128/jvi.74.1.305-311.2000

PubMed Abstract | CrossRef Full Text | Google Scholar

Marjomäki, V., Pietiäinen, V., Matilainen, H., Upla, P., Ivaska, J., Nissinen, L., et al. (2002). Internalization of Echovirus 1 in Caveolae. J. Virol. 76, 1856–1865. doi: 10.1128/jvi.76.4.1856-1865.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Marquardt, M. T., and Kielian, M. (1996). Cholesterol-depleted cells that are relatively permissive for semliki forest virus infection. Virology 224, 198–205. doi: 10.1006/viro.1996.0521

PubMed Abstract | CrossRef Full Text | Google Scholar

Martín-Acebes, M. A., González-Magaldi, M., Sandvig, K., Sobrino, F., and Armas-Portela, R. (2007). Productive entry of type C foot-and-mouth disease virus into susceptible cultured cells requires clathrin and is dependent on the presence of plasma membrane cholesterol. Virology 369, 105–118. doi: 10.1016/j.virol.2007.07.021

PubMed Abstract | CrossRef Full Text | Google Scholar

Mayle, K. M., Le, A. M., and Kamei, D. T. (2012). The intracellular trafficking pathway of Transferrin. Biochim. Biophys. Acta 1820, 264–281. doi: 10.1016/j.bbagen.2011.09.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Mayor, S., Parton, R. G., and Donaldson, J. G. (2014). Clathrin-independent pathways of endocytosis. Cold Spring Harb. Perspect. Biol. 6:a016758. doi: 10.1101/cshperspect.a016758

PubMed Abstract | CrossRef Full Text | Google Scholar

McMahon, K. A., Zajicek, H., Li, W. P., Peyton, M. J., Minna, J. D., Hernandez, V. J., et al. (2009). SRBC/cavin-3 is a caveolin adapter protein that regulates caveolae function. EMBO J. 28, 1001–1015. doi: 10.1038/emboj.2009.46

PubMed Abstract | CrossRef Full Text | Google Scholar

Medigeshi, G. R., Hirsch, A. J., Streblow, D. N., Nikolich-Zugich, J., and Nelson, J. A. (2008). West Nile Virus entry requires cholesterol-rich membrane microdomains and is independent of αvβ3 integrin. J. Virol. 82, 5212–5219.

Google Scholar

Meertens, L., Bertaux, C., and Dragic, T. (2006). Hepatitis C virus entry requires a critical postinternalization step and delivery to early endosomes via clathrin-coated vesicles. J. Virol. 80, 11571–11578.

Google Scholar

Mercer, J., and Helenius, A. (2009). Virus Entry by macropinocytosis. Nat. Cell Biol. 11, 510–520. doi: 10.1038/ncb0509-510

PubMed Abstract | CrossRef Full Text | Google Scholar

Millán, J., Cerny, J., Horejsi, V., and Alonso, M. A. (1999). CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Tissue Antigens 53, 33–40. doi: 10.1034/j.1399-0039.1999.530104.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Molotkovsky, R. J., Alexandrova, V. V., Galimzyanov, T. R., Jiménez-Munguía, I., Pavlov, K. V., Batishchev, O. V., et al. (2018). Lateral membrane heterogeneity regulates viral-induced membrane fusion during HIV entry. Int. J. Mol. Sci. 19:1483. doi: 10.3390/ijms19051483

PubMed Abstract | CrossRef Full Text | Google Scholar

Moretti, S., Procopio, A., Lazzarini, R., Rippo, M. R., Testa, R., Marra, M., et al. (2008). Semaphorin3A signaling controls Fas (CD95)-mediated apoptosis by promoting Fas translocation into lipid rafts. Blood 111, 2290–2299.

Google Scholar

Moriyama, T., Marquez, J. P., Wakatsuki, T., and Sorokin, A. (2007). Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells. J. Virol. 81, 8552–8562.

Google Scholar

Mukhamedova, N., Hoang, A., Dragoljevic, D., Dubrovsky, L., Pushkarsky, T., Low, H., et al. (2019). Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells. PLoS Pathog. 15:e1007907. doi: 10.1371/journal.ppat.1007907

PubMed Abstract | CrossRef Full Text | Google Scholar

Nanbo, A., Imai, M., Watanabe, S., Noda, T., Takahashi, K., Neumann, G., et al. (2010). Ebolavirus is internalized into host cells via macropinocytosis in a viral glycoprotein-dependent manner. PLoS Pathog. 6:e1001121. doi: 10.1371/journal.ppat.1001121

PubMed Abstract | CrossRef Full Text | Google Scholar

Narayan, S., Barnard, R. J. O., and Young, J. A. T. (2003). Two retroviral entry pathways distinguished by lipid raft association of the viral receptor and differences in viral infectivity. J. Virol. 77, 1977–1983. doi: 10.1128/jvi.77.3.1977-1983.2003

PubMed Abstract | CrossRef Full Text | Google Scholar

Navarro-Sanchez, E., Altmeyer, R., Amara, A., Schwartz, O., Fieschi, F., Virelizier, J. L., et al. (2003). Dendritic-cell-specific ICAM3-grabbing non-integrin is essential for the productive infection of human dendritic cells by mosquito-cell-derived dengue viruses. EMBO Rep. 4, 723–728. doi: 10.1038/sj.embor.embor866

PubMed Abstract | CrossRef Full Text | Google Scholar

Nawa, M., Takasaki, T., Yamada, K. I., Kurane, I., and Akatsuka, T. (2003). Interference in Japanese encephalitis virus infection of Vero cells by a cationic amphiphilic drug, chlorpromazine. J. Gen. Virol. 84, 1737–1741.

Google Scholar

Nichols, B. (2003a). Caveosomes and endocytosis of lipid rafts. J. Cell Sci. 116, 4707–4714. doi: 10.1242/jcs.00840

PubMed Abstract | CrossRef Full Text | Google Scholar

Nichols, B. J. (2003b). GM1-containing lipid rafts are depleted within clathrin-coated pits. Curr. Biol. 13, 686–690.

Google Scholar

Nicola, A. V. (2016). Herpesvirus entry into host cells mediated by endosomal low pH. Traffic 17, 965–975. doi: 10.1111/tra.12408

PubMed Abstract | CrossRef Full Text | Google Scholar

Nieva, J. L., Bron, R., Corver, J., and Wilschut, J. (1994). Membrane fusion of Semliki Forest virus requires sphingolipids in the target membrane. EMBO J. 13, 2797–2804. doi: 10.1002/j.1460-2075.1994.tb06573.x

CrossRef Full Text | Google Scholar

Ning, P., Gao, L., Zhou, Y., Hu, C., Lin, Z., Gong, C., et al. (2016). Caveolin-1-mediated endocytic pathway is involved in classical swine fever virus Shimen infection of porcine alveolar macrophages. Vet. Microbiol. 195, 81–86. doi: 10.1016/j.vetmic.2016.09.016

PubMed Abstract | CrossRef Full Text | Google Scholar

Nomura, R., Kiyota, A., Suzaki, E., Kataoka, K., Ohe, Y., Miyamoto, K., et al. (2004). Human Coronavirus 229E Binds to CD13 in Rafts and Enters the Cell through Caveolae. J. Virol. 78, 8701–8708. doi: 10.1128/jvi.78.16.8701-8708.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

Nonnenmacher, M., and Weber, T. (2011). Adeno-associated virus 2 infection requires endocytosis through the CLIC/GEEC pathway. Cell Host Microbe 10, 563–576. doi: 10.1016/j.chom.2011.10.014

PubMed Abstract | CrossRef Full Text | Google Scholar

Norkin, L. C. (1999). Simian virus 40 infection via MHC class I molecules and caveolae. Immunol. Rev. 168, 13–22. doi: 10.1111/j.1600-065X.1999.tb01279.x

PubMed Abstract | CrossRef Full Text | Google Scholar

O’Donnell, V., LaRocco, M., and Baxt, B. (2008). Heparan sulfate-binding foot-and-mouth disease virus enters cells via caveola-mediated endocytosis. J. Virol. 82, 9075–9085.

Google Scholar

Otsuki, N., Sakata, M., Saito, K., Okamoto, K., Mori, Y., Hanada, K., et al. (2017). Both Sphingomyelin and cholesterol in the host cell membrane are essential for rubella virus entry. J. Virol. 92:e01130-17.

Google Scholar

Ouweneel, A. B., Thomas, M. J., and Sorci-Thomas, M. G. (2020). The ins and outs of lipid rafts: functions in intracellular cholesterol homeostasis, microparticles and cell membranes. J. Lipid Res. 61, 676–686. doi: 10.1194/jlr.TR119000383

PubMed Abstract | CrossRef Full Text | Google Scholar

Owczarek, K., Szczepanski, A., Milewska, A., Baster, Z., Rajfur, Z., Sarna, M., et al. (2018). Early events during human coronavirus OC43 entry to the cell. Sci. Rep. 8:7124.

Google Scholar

Parton, R. G., and Lindsay, M. (1999). Exploitation of Major Histocompatibility Complex Class I Molecules and Caveolae by Simian Virus 40. Immunol. Rev. 168, 23–31. doi: 10.1111/j.1600-065X.1999.tb01280.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Pastenkos, G., Miller, J. L., Pritchard, S. M., and Nicola, A. V. (2018). Role of sphingomyelin in alphaherpesvirus entry. J. Virol. 93:e01547-18.

Google Scholar

Pelkmans, L., Bü, T., Zerial, M., and Helenius, A. (2004). Caveolin-stabilized membrane domains as multifunctional transport and sorting devices in endocytic membrane traffic. Cell 118, 767–780. doi: 10.1016/j.cell.2004.09.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Pelkmans, L., Kartenbeck, J., and Helenius, A. (2001). Caveolar endocytosis of simian virus 40 reveals a new two-step vesicular-transport pathway to the ER. Nat. Cell Biol. 3, 473–483. doi: 10.1038/35074539

PubMed Abstract | CrossRef Full Text | Google Scholar

Percherancier, Y., Lagane, B., Planchenault, T., Staropoli, I., Altmeyer, R., Virelizier, J. L., et al. (2003). HIV-1 entry into T-cells is not dependent on CD4 and CCR5 localization to sphingolipid-enriched, detergent-resistant, raft membrane domains. J. Biol. Chem. 278, 3153–3161. doi: 10.1074/jbc.M207371200

PubMed Abstract | CrossRef Full Text | Google Scholar

Phalen, T., and Kielian, M. (1991). Cholesterol is required for infection by semliki forest virus. J. Cell Biol. 112, 615–623. doi: 10.1083/jcb.112.4.615

PubMed Abstract | CrossRef Full Text | Google Scholar

Pietiäinen, V., Marjomäki, V., Upla, P., Pelkmans, L., Helenius, A., and Hyypiä, T. (2004). Echovirus 1 endocytosis into caveosomes requires lipid rafts, dynamin II, and signaling events. Mol. Biol. Cell 15, 4911–4925.

Google Scholar

Pike, L. J. (2006). Rafts defined: a report on the Keystone symposium on lipid rafts and cell function. J. Lipid Res. 47, 1597–1598. doi: 10.1194/jlr.E600002-JLR200

PubMed Abstract | CrossRef Full Text | Google Scholar

Popik, W., Alce, T. M., and Au, W.-C. (2002). Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4+ T cells. J. Virol. 76, 4709–4722. doi: 10.1128/jvi.76.10.4709-4722.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Praena, B., Bello-Morales, R., and Antonio López-Guerrero, J. (2020). Hsv-1 endocytic entry into a human oligodendrocytic cell line is mediated by clathrin and dynamin but not caveolin. Viruses 12:734. doi: 10.3390/v12070734

PubMed Abstract | CrossRef Full Text | Google Scholar

Puri, A., Hug, P., Jernigan, K., Barchi, J., Kim, H. Y., Hamilton, J., et al. (1998). The neutral glycosphingolipid globotriaosylceramide promotes fusion mediated by a CD4-dependent CXCR4-utilizing HIV type 1 envelope glycoprotein. Proc. Natl. Acad. Sci. U.S.A. 95, 14435–14440. doi: 10.1073/pnas.95.24.14435

PubMed Abstract | CrossRef Full Text | Google Scholar

Querbes, W., O’Hara, B. A., Williams, G., and Atwood, W. J. (2006). Invasion of host cells by JC Virus identifies a novel role for caveolae in endosomal sorting of Noncaveolar Ligands. J. Virol. 80, 9402–9413.

Google Scholar

Quirin, K., Eschli, B., Scheu, I., Poort, L., Kartenbeck, J., and Helenius, A. (2008). Lymphocytic choriomeningitis virus uses a novel endocytic pathway for infectious entry via late endosomes. Virology 378, 21–33. doi: 10.1016/j.virol.2008.04.046

PubMed Abstract | CrossRef Full Text | Google Scholar

Raghu, H., Sharma-Walia, N., Veettil, M. V., Sadagopan, S., Caballero, A., Sivakumar, R., et al. (2007). Lipid rafts of primary endothelial cells are essential for Kaposi’s Sarcoma-Associated Herpesvirus/Human Herpesvirus 8-Induced Phosphatidylinositol 3-Kinase and RhoA-GTPases critical for microtubule dynamics and nuclear delivery of viral DNA but Dispensab. J. Virol. 81, 7941–7959.

Google Scholar

Rawat, S. S., Eaton, J., Gallo, S. A., Martin, T. D., Ablan, S., Ratnayake, S., et al. (2004). Functional expression of CD4, CXCR4, and CCR5 in glycosphingolipid- deficient mouse melanoma GM95 cells and susceptibility to HIV-1 envelope glycoprotein-triggered membrane fusion. Virology 318, 55–65. doi: 10.1016/j.virol.2003.08.042

PubMed Abstract | CrossRef Full Text | Google Scholar

Rawat, S. S., Viard, M., Gallo, S. A., Blumenthal, R., and Puri, A. (2006). Sphingolipids, cholesterol, and HIV-1: A paradigm in viral fusion. Glycoconj. J. 23, 189–197.

Google Scholar

Rawat, S. S., Viard, M., Gallo, S. A., Rein, A., Blumenthal, R., and Puri, A. (2003). Modulation of entry of enveloped viruses by cholesterol and sphingolipids. Mol. Membr. Biol. 20, 243–254. doi: 10.1080/0968768031000104944

PubMed Abstract | CrossRef Full Text | Google Scholar

Reyes-del Valle, J., Chávez-Salinas, S., Medina, F., and del Angel, R. M. (2005). Heat shock protein 90 and heat shock protein 70 are components of dengue virus receptor complex in human cells. J. Virol. 79, 4557–4567. doi: 10.1128/jvi.79.8.4557-4567.2005

PubMed Abstract | CrossRef Full Text | Google Scholar

Rhainds, D., Bourgeois, P., Bourret, G., Huard, K., Falstrault, L., and Brissette, L. (2004). Localization and regulation of SR-BI in membrane rafts of HepG2 cells. J. Cell Sci. 117, 3095–3105. doi: 10.1242/jcs.01182

PubMed Abstract | CrossRef Full Text | Google Scholar

Richterová, Z., Liebl, D., Horák, M., Palková, Z., Štokrová, J., Hozák, P., et al. (2001). Caveolae are involved in the trafficking of mouse polyomavirus virions and artificial VP1 pseudocapsids toward cell nuclei. J. Virol. 75, 10880–10891. doi: 10.1128/jvi.75.22.10880-10891.2001

PubMed Abstract | CrossRef Full Text | Google Scholar

Risselada, H. J. (2017). Membrane fusion stalks and lipid rafts: a love-hate relationship. Biophys. J. 112, 2475–2478. doi: 10.1016/j.bpj.2017.04.031

PubMed Abstract | CrossRef Full Text | Google Scholar

Rodal, S. K., Skretting, G., Garred, Ø., Vilhardt, F., Van Deurs, B., and Sandvig, K. (1999). Extraction of cholesterol with Methyl-β-cyclodextrin perturbs formation of clathrin-coated endocytic vesicles. Mol. Biol. Cell 10, 961–974. doi: 10.1091/mbc.10.4.961

PubMed Abstract | CrossRef Full Text | Google Scholar

Rogée, S., Grellier, E., Bernard, C., Loyens, A., Beauvillain, J. C., D’halluin, J. C., et al. (2007). Intracellular trafficking of a fiber-modified adenovirus using lipid raft/caveolae endocytosis. Mol. Ther. 15, 1963–1972. doi: 10.1038/sj.mt.6300283

PubMed Abstract | CrossRef Full Text | Google Scholar

Rojek, J. M., Perez, M., and Kunz, S. (2008). Cellular entry of lymphocytic choriomeningitis virus. J. Virol. 82, 1505–1517.

Google Scholar

Rollason, R., Korolchuk, V., Hamilton, C., Schu, P., and Banting, G. (2007). Clathrin-mediated endocytosis of a lipid-raft-associated protein is mediated through a dual tyrosine motif. J. Cell Sci. 120, 3850–3858. doi: 10.1242/jcs.003343

PubMed Abstract | CrossRef Full Text | Google Scholar

Rust, M. J., Lakadamyali, M., Zhang, F., and Zhuang, X. (2004). Assembly of endocytic machinery around individual influenza viruses during viral entry. Nat. Struct. Mol. Biol. 11, 567–573. doi: 10.1038/nsmb769

PubMed Abstract | CrossRef Full Text | Google Scholar

Saeed, M. F., Kolokoltsov, A. A., Albrecht, T., and Davey, R. A. (2010). Cellular entry of Ebola virus involves uptake by a macropinocytosis-like mechanism and subsequent trafficking through early and late endosomes. PLoS Pathog. 6:e1001110. doi: 10.1371/journal.ppat.1001110

PubMed Abstract | CrossRef Full Text | Google Scholar

Sáez-Cirión, A., Nir, S., Lorizate, M., Agirre, A., Cruz, A., Pérez-Gil, J., et al. (2002). Sphingomyelin and cholesterol promote HIV-1 gp41 pretransmembrane sequence surface aggregation and membrane restructuring. J. Biol. Chem. 277, 21776–21785. doi: 10.1074/jbc.M202255200

PubMed Abstract | CrossRef Full Text | Google Scholar

Salikhova, A., Wang, L., Lanahan, A., Liu, M., Simons, M., Leenders, W., et al. (2008). Vascular endothelial growth factor and semaphorin induce neuropilin-1 endocytosis via separate pathways. Circ. Res. 103, e71–e79. doi: 10.1161/CIRCULATIONAHA.110.956839

PubMed Abstract | CrossRef Full Text | Google Scholar

Sanchez, A. (2007). Analysis of Filovirus entry into vero E6 cells, using inhibitors of endocytosis, endosomal acidification, structural integrity, and cathepsin (B and L) activity. J. Infect. Dis. 196, S251–S258. doi: 10.1086/520597

PubMed Abstract | CrossRef Full Text | Google Scholar

Sánchez-San Martín, C., López, T., Arias, C. F., and López, S. (2004). Characterization of rotavirus cell entry. J. Virol. 78, 2310–2318. doi: 10.1128/jvi.78.5.2310-2318.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

Sandvig, K., Kavaliauskiene, S., and Skotland, T. (2018). Clathrin-independent endocytosis: an increasing degree of complexity. Histochem. Cell Biol. 150, 107–118.

Google Scholar

Sarnataro, D., Caputo, A., Casanova, P., Puri, C., Paladino, S., Tivodar, S. S., et al. (2009). Lipid rafts and clathrin cooperate in the internalization of PrPC in epithelial FRT cells. PLoS One 4:e5829. doi: 10.1371/journal.pone.0005829

PubMed Abstract | CrossRef Full Text | Google Scholar

Sato, S. B., Ishii, K., Makino, A., Iwabuchi, K., Yamaji-Hasegawa, A., Senoh, Y., et al. (2004). Distribution and transport of cholesterol-rich membrane domains monitored by a membrane-impermeant fluorescent polyethylene glycol-derivatizied cholesterol. J. Biol. Chem. 279, 23790–23796. doi: 10.1074/jbc.M313568200

PubMed Abstract | CrossRef Full Text | Google Scholar

Scarselli, E., Ansuini, H., Cerino, R., Roccasecca, R. M., Acali, S., Filocamo, G., et al. (2002). The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus. EMBO J. 21, 5017–5025. doi: 10.1093/emboj/cdf529

PubMed Abstract | CrossRef Full Text | Google Scholar

Schroeder, N., Chung, C.-S., Chen, C.-H., Liao, C.-L., and Chang, W. (2012). The lipid raft-associated protein CD98 is required for Vaccinia Virus endocytosis. J. Virol. 86, 4868–4882.

Google Scholar

Sezgin, E., Levental, I., Mayor, S., and Eggeling, C. (2017). The mystery of membrane organization: composition, regulation and roles of lipid rafts. Nat. Rev. Mol. Cell Biol. 18, 361–374. doi: 10.1038/nrm.2017.16

PubMed Abstract | CrossRef Full Text | Google Scholar

Shafaq-Zadah, M., Dransart, E., and Johannes, L. (2020). Clathrin-independent endocytosis, retrograde trafficking, and cell polarity. Curr. Opin. Cell Biol. 65, 112–121. doi: 10.1016/j.ceb.2020.05.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Shah, W. A., Peng, H., and Carbonetto, S. (2006). Role of non-raft cholesterol in lymphocytic choriomeningitis virus infection via α-dystroglycan. J. Gen. Virol. 87, 673–678.

Google Scholar

Shi, Y., and Ruan, H. (2020). Toward a membrane-centric biology. Front. Immunol. 11:1909. doi: 10.3389/fimmu.2020.01909

PubMed Abstract | CrossRef Full Text | Google Scholar

Sieczkarski, S. B., and Whittaker, G. R. (2002). Influenza virus can enter and infect cells in the absence of clathrin-mediated endocytosis. J. Virol. 76, 10455–10464. doi: 10.1128/jvi.76.20.10455-10464.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Sieczkarski, S. B., and Whittaker, G. R. (2004). “Viral entry,” in Membrane Trafficking in Viral Replication. Current Topics in Microbiology and Immunology, Vol. 285, ed. M. Marsh (Berlin: Springer).

Google Scholar

Simon, M., Johansson, C., and Mirazimi, A. (2009). Crimean-Congo hemorrhagic fever virus entry and replication is clathrin-, pH- and cholesterol- dependent. J. Gen. Virol. 90, 210–215.

Google Scholar

Singer, S. J., and Nicolson, G. L. (1972). The fluid mosaic model of the structure of cell membranes. Science 175, 720–731. doi: 10.1126/science.175.4023.720

PubMed Abstract | CrossRef Full Text | Google Scholar

Singh, M., Jadhav, H. R., and Bhatt, T. (2017). Dynamin functions and ligands: classical mechanisms behind. Mol. Pharmacol. 91, 123–134. doi: 10.1124/mol.116.105064

PubMed Abstract | CrossRef Full Text | Google Scholar

Skryabin, G. O., Komelkov, A. V., Savelyeva, E. E., and Tchevkina, E. M. (2020). Lipid rafts in exosome biogenesis. Biochemistry 85, 177–191. doi: 10.1134/S0006297920020054

PubMed Abstract | CrossRef Full Text | Google Scholar

Smit, J. M., Bittman, R., and Wilschut, J. (1999). Low-pH-dependent fusion of sindbis virus with receptor-free cholesterol- and sphingolipid-containing liposomes. J. Virol. 73, 8476–8484. doi: 10.1128/jvi.73.10.8476-8484.1999

PubMed Abstract | CrossRef Full Text | Google Scholar

Smith, J. L., Campos, S. K., and Ozbun, M. A. (2007). Human papillomavirus type 31 uses a caveolin 1- and Dynamin 2-mediated entry pathway for infection of human keratinocytes. J. Virol. 81, 9922–9931.

Google Scholar

Smith, J. L., Campos, S. K., Wandinger-Ness, A., and Ozbun, M. A. (2008). Caveolin-1-dependent infectious entry of human papillomavirus Type 31 in human keratinocytes proceeds to the endosomal pathway for pH-dependent uncoating. J. Virol. 82, 9505–9512.

Google Scholar

Soldaini, E., Wack, A., D’Oro, U., Nuti, S., Ulivieri, C., Baldari, C. T., et al. (2003). T cell costimulation by the hepatitis C virus envelope protein E2 binding to CD81 is mediated by Lck. Eur. J. Immunol. 33, 455–464. doi: 10.1002/immu.200310021

PubMed Abstract | CrossRef Full Text | Google Scholar

Stang, E., Kartenbeck, J., and Parton, R. G. (1997). Major histocompatibility complex class I molecules mediate association of SV40 with caveolae. Mol. Biol. Cell 8, 47–57. doi: 10.1091/mbc.8.1.47

PubMed Abstract | CrossRef Full Text | Google Scholar

Stoddart, A., Dykstra, M. L., Brown, B. K., Song, W., Pierce, S. K., and Brodsky, F. M. (2002). Lipid rafts unite signaling cascades with clathrin to regulate BCR internalization. Immunity 17, 451–462.

Google Scholar

Stuart, A. D., Eustace, H. E., McKee, T. A., and Brown, T. D. K. (2002). A novel cell entry pathway for a DAF-using human enterovirus is dependent on lipid rafts. J. Virol. 76, 9307–9322. doi: 10.1128/jvi.76.18.9307-9322.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Subtil, A., Gaidarov, I., Kobylarz, K., Lampson, M. A., Keen, J. H., and Mcgraw, T. E. (1999). Acute cholesterol depletion inhibits clathrin-coated pit budding. Proc. Natl. Acad. Sci. U.S.A. 96, 6775–6780. doi: 10.1073/pnas.96.12.6775

PubMed Abstract | CrossRef Full Text | Google Scholar

Suomalainen, M. (2002). Lipid rafts and assembly of enveloped viruses. Traffic 3, 705–709. doi: 10.1034/j.1600-0854.2002.31002.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Szczepanski, A., Owczarek, K., Milewska, A., Baster, Z., Rajfur, Z., Mitchell, J. A., et al. (2018). Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells. Vet. Res. 49:55.

Google Scholar

Szklarczyk, O. M., González-Segredo, N., Kukura, P., Oppenheim, A., Choquet, D., Sandoghdar, V., et al. (2013). Receptor concentration and diffusivity control multivalent binding of Sv40 to membrane bilayers. PLoS Comput. Biol. 9:e1003310. doi: 10.1371/journal.pcbi.1003310

PubMed Abstract | CrossRef Full Text | Google Scholar

Takahashi, T., and Suzuki, T. (2009). Role of membrane rafts in viral infection. Open Dermatol. J. 3, 178–194. doi: 10.2174/1874372200903010178

CrossRef Full Text | Google Scholar

Takahashi, T., and Suzuki, T. (2011). Function of membrane rafts in viral lifecycles and host cellular response. Biochem. Res. Int. 2011:245090. doi: 10.1155/2011/245090

PubMed Abstract | CrossRef Full Text | Google Scholar

Tang, H., Kawabata, A., Takemoto, M., Yamanishi, K., and Mori, Y. (2008). Human herpesvirus-6 infection induces the reorganization of membrane microdomains in target cells, which are required for virus entry. Virology 378, 265–271. doi: 10.1016/j.virol.2008.05.028

PubMed Abstract | CrossRef Full Text | Google Scholar

Tang, H., and Mori, Y. (2010). Human herpesvirus-6 entry into host cells. Future Microbiol. 5, 1015–1023. doi: 10.2217/fmb.10.61

PubMed Abstract | CrossRef Full Text | Google Scholar

Tang, Q., Liu, P., Chen, M., and Qin, Y. (2019). Virion-associated cholesterol regulates the infection of human Parainfluenza Virus type 3. Viruses 11:438. doi: 10.3390/v11050438

PubMed Abstract | CrossRef Full Text | Google Scholar

Tani, H., Shiokawa, M., Kaname, Y., Kambara, H., Mori, Y., Abe, T., et al. (2010). Involvement of ceramide in the propagation of japanese encephalitis virus. J. Virol. 84, 2798–2807.

Google Scholar

Tassaneetrithep, B., Burgess, T. H., Granelli-Piperno, A., Trumpfheller, C., Finke, J., Sun, W., et al. (2003). DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. J. Exp. Med. 197, 823–829. doi: 10.1084/jem.20021840

PubMed Abstract | CrossRef Full Text | Google Scholar

Thorp, E. B., and Gallagher, T. M. (2004). Requirements for CEACAMs and cholesterol during Murine Coronavirus cell entry. J. Virol. 78, 2682–2692. doi: 10.1128/jvi.78.6.2682-2692.2004

PubMed Abstract | CrossRef Full Text | Google Scholar

Toscano, M. G., and de Haan, P. (2018). How Simian Virus 40 Hijacks the intracellular protein trafficking pathway to Its Own Benefit … and Ours. Front. Immunol. 9:1160. doi: 10.3389/fimmu.2018.01160

PubMed Abstract | CrossRef Full Text | Google Scholar

Triantafilou, K., and Triantafilou, M. (2003). Lipid raft microdomains: key sites for Coxsackievirus A9 infectious cycle. Virology 317, 128–135. doi: 10.1016/j.virol.2003.08.036

PubMed Abstract | CrossRef Full Text | Google Scholar

Triantafilou, K., and Triantafilou, M. (2004). Lipid-raft-dependent Coxsackievirus B4 internalization and rapid targeting to the Golgi. Virology 326, 6–19. doi: 10.1016/j.virol.2004.04.051

PubMed Abstract | CrossRef Full Text | Google Scholar

Tsai, B., Gilbert, J. M., Stehle, T., Lencer, W., Benjamin, T. L., and Rapoport, T. A. (2003). Gangliosides are receptors for Murine Polyoma Virus and SV40. Eur. Mol. Biol. Organ. J. 22, 4346–4355. doi: 10.1093/emboj/cdg439

PubMed Abstract | CrossRef Full Text | Google Scholar

Upla, P., Marjomäki, V., Kankaanpää, P., Ivaska, J., Hyypiä, T., Van Der Goot, F. G., et al. (2004). Clustering induces a lateral redistribution of α2β1 integrin from membrane rafts to caveolae and subsequent protein kinase C-dependent internalization. Mol. Biol. Cell 15, 625–636.

Google Scholar

van IJzendoorn, S. C. D., Agnetti, J., and Gassama-Diagne, A. (2020). Mechanisms behind the polarized distribution of lipids in epithelial cells. Biochim. Biophys. Acta Biomembr. 1862:183145. doi: 10.1016/j.bbamem.2019.183145

PubMed Abstract | CrossRef Full Text | Google Scholar

Van Wilgenburg, B., Moore, M. D., James, W. S., and Cowley, S. A. (2014). The productive entry pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts containing CD4. PLoS One 9:e86071. doi: 10.1371/journal.pone.0086071

PubMed Abstract | CrossRef Full Text | Google Scholar

VanHamme, E., Dewerchin, H. L., Cornelissen, E., Verhasselt, B., and Nauwynck, H. J. (2008). Clathrin- and caveolae-independent entry of feline infectious peritonitis virus in monocytes depends on dynamin. J. Gen. Virol. 89, 2147–2156.

Google Scholar

Varshney, P., Yadav, V., and Saini, N. (2016). Lipid rafts in immune signalling: current progress and future perspective. Immunology 149, 13–24. doi: 10.1111/imm.12617

PubMed Abstract | CrossRef Full Text | Google Scholar

Veetti, M. V., Sadagopan, S., Kerur, N., Chakraborty, S., and Chandran, B. (2010). Interaction of c-Cbl with Myosin IIA Regulates Bleb associated macropinocytosis of Kaposi’s sarcoma-associated herpesvirus. PLoS Pathog. 6:e1001238. doi: 10.1371/journal.ppat.1001238

PubMed Abstract | CrossRef Full Text | Google Scholar

Veettil, M. V., Bandyopadhyay, C., Dutta, D., and Chandran, B. (2014). Interaction of KSHV with host cell surface receptors and cell entry. Viruses 6, 4024–4046. doi: 10.3390/v6104024

PubMed Abstract | CrossRef Full Text | Google Scholar

Verma, D. K., Gupta, D., and Lal, S. K. (2018). Host lipid rafts play a major role in binding and endocytosis of influenza a virus. Viruses 10:650. doi: 10.3390/v10110650

PubMed Abstract | CrossRef Full Text | Google Scholar

Viard, M., Parolini, I., Rawat, S. S., Fecchi, K., Sargiacomo, M., Puri, A., et al. (2004). The role of glycosphingolipids in HIV signaling, entry and pathogenesis. J. Bus. Ethics 51, 31–39. doi: 10.1023/B

CrossRef Full Text | Google Scholar

Viard, M., Parolini, I., Sargiacomo, M., Fecchi, K., Ramoni, C., Ablan, S., et al. (2002). Role of cholesterol in human immunodeficiency virus type 1 envelope protein-mediated fusion with host cells. J. Virol. 76, 11584–11595. doi: 10.1128/jvi.76.22.11584-11595.2002

PubMed Abstract | CrossRef Full Text | Google Scholar

Vidricaire, G., and Tremblay, M. J. (2007). A clathrin, caveolae, and dynamin-independent endocytic pathway requiring free membrane cholesterol drives HIV-1 internalization and infection in polarized trophoblastic cells. J. Mol. Biol 368, 1267–1283. doi: 10.1016/j.jmb.2007.03.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Vitiello, G., Falanga, A., Petruk, A. A., Merlino, A., Fragneto, G., Paduano, L., et al. (2015). Fusion of raft-like lipid bilayers operated by a membranotropic domain of the HSV-type I glycoprotein gH occurs through a cholesterol-dependent mechanism. Soft Matter 11, 3003–3016. doi: 10.1039/c4sm02769h

PubMed Abstract | CrossRef Full Text | Google Scholar

Voisset, C., Lavie, M., Helle, F., Op De Beeck, A., Bilheu, A., Bertrand-Michel, J., et al. (2008). Ceramide enrichment of the plasma membrane induces CD81 internalization and inhibits hepatitis C virus entry. Cell. Microbiol. 10, 606–617. doi: 10.1111/j.1462-5822.2007.01070.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Waarts, B. L., Bittman, R., and Wilschut, J. (2002). Sphingolipid and cholesterol dependence of alphavirus membrane fusion: lack of correlation with lipid raft formation in target liposomes. J. Biol. Chem. 277, 38141–38147. doi: 10.1074/jbc.M206998200

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, H., Sun, Y., Mao, X., Meng, C., Tan, L., Qiu, X., et al. (2019a). Infectious bronchitis virus attaches to lipid rafts and enters cells via clathrin mediated endocytosis. Virology 528, 118–136. doi: 10.1101/352898

CrossRef Full Text | Google Scholar

Wang, H., Yuan, X., Sun, Y., Mao, X., Meng, C., Tan, L., et al. (2019b). Infectious bronchitis virus entry mainly depends on clathrin mediated endocytosis and requires classical endosomal/lysosomal system. Virology 528, 118–136. doi: 10.1016/j.virol.2018.12.012

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, H., Yang, P., Liu, K., Guo, F., Zhang, Y., Zhang, G., et al. (2008). SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway. Cell Res. 18, 290–301. doi: 10.1038/cr.2008.15

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, H., Yuan, Z., Pavel, M. A., and Hansen, S. (2020). Cholesterol and COVID19 lethality in elderly. bioRxiv [Preprint]. doi: 10.1101/2020.05.09.086249

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, J., Li, Y., Wang, S., and Liu, F. (2020). Dynamics of transmissible gastroenteritis virus internalization unraveled by single-virus tracking in live cells. FASEB J. 34, 4653–4669. doi: 10.1096/fj.201902455R

CrossRef Full Text | Google Scholar

Wang, Y., Zhang, Y., Zhang, C., Hu, M., Yan, Q., Zhao, H., et al. (2020). Cholesterol-rich lipid rafts in the cellular membrane play an essential role in avian Reovirus replication. Front. Microbiol. 11:597794. doi: 10.3389/fmicb.2020.597794

PubMed Abstract | CrossRef Full Text | Google Scholar

Weed, D. J., and Nicola, A. V. (2017). Herpes simplex virus membrane fusion. Adv. Anat. Embryol. Cell Biol. 223, 29–47. doi: 10.1007/978-3-319-53168-7_2

CrossRef Full Text | Google Scholar

Wei, X., She, G., Wu, T., Xue, C., and Cao, Y. (2020). PEDV enters cells through clathrin-, caveolae-, and lipid raft-mediated endocytosis and traffics via the endo-/lysosome pathway. Vet. Res. 51:10.

Google Scholar

Wielgosz, M. M., Rauch, D. A., Jones, K. S., Ruscetti, F. W., and Ratner, L. (2005). Cholesterol dependence of HTLV-I infection. AIDS Res. Hum. Retroviruses 21, 43–50. doi: 10.1089/aid.2005.21.43

PubMed Abstract | CrossRef Full Text | Google Scholar

Williams, T. M., and Lisanti, M. P. (2004). The caveolin proteins. Genome Biol. 5:214.

Google Scholar

Xing, Y., Wen, Z., Gao, W., Lin, Z., Zhong, J., and Jiu, Y. (2020). Multifaceted functions of host cell caveolae/caveolin-1 in virus infections. Viruses 12:487. doi: 10.3390/v12050487

CrossRef Full Text | Google Scholar

Yamauchi, Y., and Helenius, A. (2013). Virus entry at a glance. J. Cell Sci. 126, 1289–1295. doi: 10.1242/jcs.119685

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, S., He, M., Liu, X., Li, X., Fan, B., and Zhao, S. (2013). Japanese encephalitis virus infects porcine kidney epithelial PK15 cells via clathrin- and cholesterol-dependent endocytosis. Virol. J. 10:258.

Google Scholar

Yang, S.-T., Kiessling, V., Simmons, J. A., White, J. M., and Tamm, L. K. (2017). HIV gp41-mediated membrane fusion occurs at edges of cholesterol-rich lipid domains. Nat. Chem. Biol. 11, 424–431. doi: 10.1038/nchembio.1800.HIV

CrossRef Full Text | Google Scholar

Yang, S.-T., Kiessling, V., and Tamm, L. (2016). Line tension at lipid phase boundaries as drivind force for HIV fusion peptide-mediated fusion. Nat. Commun. 7:11401. doi: 10.1038/pj.2016.37

CrossRef Full Text | Google Scholar

Yasen, A., Herrera, R., Rosbe, K., Lien, K., and Tugizov, S. M. (2018). HIV internalization into oral and genital epithelial cells by endocytosis and macropinocytosis leads to viral sequestration in the vesicles. Virology 515, 92–107. doi: 10.1016/j.physbeh.2017.03.040

PubMed Abstract | CrossRef Full Text | Google Scholar

Yousuf, M. A., Zhou, X., Mukherjee, S., Chintakuntlawar, A. V., Lee, J. Y., Ramke, M., et al. (2013). Caveolin-1 associated adenovirus entry into human corneal cells. PLoS One 8:e77462. doi: 10.1371/journal.pone.0077462

PubMed Abstract | CrossRef Full Text | Google Scholar

Yurtsever, D., and Lorent, J. H. (2020). Structural modifications controlling membrane raft partitioning and curvature in human and viral proteins. J. Phys. Chem. 124, 7574–7585. doi: 10.1021/acs.jpcb.0c03435

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, F., Guo, H., Zhang, J., Chen, Q., and Fang, Q. (2018a). Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus. Virology 513, 195–207. doi: 10.1016/j.virol.2017.09.019

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, Y.-N., Liu, Y.-Y., Xiao, F.-C., Liu, C.-C., Liang, X.-D., Chen, J., et al. (2018b). Rab5, Rab7, and Rab11 are required for caveola-dependent endocytosis of classical swine fever virus in porcine alveolar macrophages. J. Virol. 92:e00797-18.

Google Scholar

Zheng, Y. H., Plemenitas, A., Linnemann, T., Fackler, O. T., and Peterlin, B. M. (2001). Nef increases infectivity of HIV via lipid rafts. Curr. Biol. 11, 875–879.

Google Scholar

Zhu, Y. Z., Wu, D. G., Ren, H., Xu, Q. Q., Zheng, K. C., Chen, W., et al. (2015). The role of lipid rafts in the early stage of enterovirus 71 infection. Cell. Physiol. Biochem. 35, 1347–1359. doi: 10.1159/000373956

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhu, Y.-Z., Xu, Q.-Q., Wu, D.-G., Ren, H., Zhao, P., Lao, W.-G., et al. (2012a). Japanese encephalitis virus enters rat neuroblastoma cells via a pH-Dependent, dynamin and caveola-mediated endocytosis pathway. J. Virol. 86, 13407–13422.

Google Scholar

Zhu, Y. Z., Cao, M. M., Wang, W. B., Wang, W., Ren, H., Zhao, P., et al. (2012b). Association of heat-shock protein 70 with lipid rafts is required for Japanese encephalitis virus infection in Huh7 cells. J. Gen. Virol. 93, 61–71.

Google Scholar

Keywords: cholesterol, viral entry, endocytosis, caveolae, raft

Citation: Ripa I, Andreu S, López-Guerrero JA and Bello-Morales R (2021) Membrane Rafts: Portals for Viral Entry. Front. Microbiol. 12:631274. doi: 10.3389/fmicb.2021.631274

Received: 19 November 2020; Accepted: 14 January 2021;
Published: 04 February 2021.

Edited by:

Julie Lucifora, Institut National de la Santé et de la Recherche Médicale (INSERM), France

Reviewed by:

Eloi R. Verrier, INSERM UMR_S1110 Institute de Recherche sur les Maladies Virales et Hepatiques, France
Dimitri L. Lavillette, Institut Pasteur of Shanghai (CAS), China

Copyright © 2021 Ripa, Andreu, López-Guerrero and Bello-Morales. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Inés Ripa, aW5lcy5yaXBhQGNibS5jc2ljLmVz

Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.