AUTHOR=Qian Hengwei , Wang Lulu , Ma Xianliang , Yi Xingling , Wang Baoshan , Liang Wenxing 

TITLE=Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylated Proteins in Fusarium oxysporum

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.623735

DOI=10.3389/fmicb.2021.623735

ISSN=1664-302X

ABSTRACT=<p>Protein lysine 2-hydroxyisobutyrylation (K<sub><italic>hib</italic></sub>), a new type of post-translational modification, occurs in histones and non-histone proteins and plays an important role in almost all aspects of both eukaryotic and prokaryotic living cells. <italic>Fusarium oxysporum</italic>, a soil-borne fungal pathogen, can cause disease in more than 150 plants. However, little is currently known about the functions of K<sub><italic>hib</italic></sub> in this plant pathogenic fungus. Here, we report a systematic analysis of 2-hydroxyisobutyrylated proteins in <italic>F. oxysporum</italic>. In this study, 3782 K<sub><italic>hib</italic></sub> sites in 1299 proteins were identified in <italic>F. oxysporum</italic>. The bioinformatics analysis showed that 2-hydroxyisobutyrylated proteins are involved in different biological processes and functions and are located in diverse subcellular localizations. The enrichment analysis revealed that K<sub><italic>hib</italic></sub> participates in a variety of pathways, including the ribosome, oxidative phosphorylation, and proteasome pathways. The protein interaction network analysis showed that 2-hydroxyisobutyrylated protein complexes are involved in diverse interactions. Notably, several 2-hydroxyisobutyrylated proteins, including three kinds of protein kinases, were involved in the virulence or conidiation of <italic>F. oxysporum</italic>, suggesting that K<sub><italic>hib</italic></sub> plays regulatory roles in pathogenesis. Moreover, our study shows that there are different K<sub><italic>hib</italic></sub> levels of <italic>F. oxysporum</italic> in conidial and mycelial stages. These findings provide evidence of K<sub><italic>hib</italic></sub> in <italic>F. oxysporum</italic>, an important filamentous plant pathogenic fungus, and serve as a resource for further exploration of the potential functions of K<sub><italic>hib</italic></sub> in <italic>Fusarium</italic> species and other filamentous pathogenic fungi.</p>