AUTHOR=Yin Qiaoxiu , Yang Rui , Ren Yafeng , Yang Zhiying , Li Tao , Huang Honglin , Tang Qin , Li Dongxue , Jiang Shilong , Wu Xian , Wang Delu , Chen Zhuo 

TITLE=Transcriptomic, Biochemical, and Morphological Study Reveals the Mechanism of Inhibition of Pseudopestalotiopsis camelliae-sinensis by Phenazine-1-Carboxylic Acid

JOURNAL=Frontiers in Microbiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.618476

DOI=10.3389/fmicb.2021.618476

ISSN=1664-302X

ABSTRACT=<p>Gray blight disease is one of the most destructive diseases of tea plants and occurs widely in the tea-growing areas of the world. It is caused by several fungal phytopathogens, of which <italic>Pseudopestalotiopsis camelliae-sinensis</italic> is the main pathogen in China. The environmentally friendly antimicrobial, phenazine-1-carboxylic acid (PCA), a metabolite of the natural soil-borne bacteria <italic>Pseudomonas</italic> spp., can inhibit a range of fungal crop diseases. In this study, we determined that PCA was active against <italic>Ps. camelliae-sinensis in vitro.</italic> We studied the mode of action of PCA on hyphae using a microscopic investigation, transcriptomics, biochemical methods, and molecular docking. The results of scanning and transmission electron microscopy indicated that PCA caused developmental deformity of mycelia and organelle damage, and it significantly decreased the accumulation of exopolysaccharides on the hyphal surface. The transcriptome revealed that 1705 and 1683 differentially expressed genes of <italic>Ps. camelliae-sinensis</italic> treated with PCA were up-regulated or down-regulated, respectively, with genes associated with ribosome biogenesis, oxidative phosphorylation, and encoding various proteins of <italic>N</italic>-glycan biosynthesis being significantly up-regulated. Up-regulation of nine genes related to <italic>N</italic>-glycan biosynthesis of <italic>Ps. camelliae-sinensis</italic> in response to PCA treatment was confirmed by reverse transcription qPCR. The enzymatic activity of catalase and superoxide dismutase of hyphae was significantly decreased by PCA treatment. Our results indicated that exposure to PCA resulted in expression changes in oxidoreductase genes, accumulation of reactive oxygen species, and decreased activity of catalase, with concomitant damage to the fungal cell membrane and cell wall.</p>