AUTHOR=Du Xiaoli , Wang Mengyu , Zhou Haijian , Li Zhenpeng , Xu Jialiang , Li Zhe , Kan Biao , Chen Daoli , Wang Xiaoli , Jin Yujuan , Ren Yan , Ma Yanping , Liu Jiuyin , Luan Yang , Cui Zhigang , Lu Xin 

TITLE=Comparison of the Multiple Platforms to Identify Various Aeromonas Species

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.625961

DOI=10.3389/fmicb.2020.625961

ISSN=1664-302X

ABSTRACT=<p>We compared several identification methods for <italic>Aeromonas</italic> genus members, including traditional biochemical testing, multiplex-PCR amplification, mass spectrometry identification, whole-genome sequencing, multilocus phylogenetic analysis (MLPA), and <italic>rpoD, gyrA</italic>, and <italic>rpoD</italic>-<italic>gyrA</italic> gene sequencing. Isolates (<italic>n</italic> = 62) belonging to the <italic>Aeromonas</italic> genus, which were came from the bacterial bank in the laboratory, were used to assess the identification accuracy of the different methods. Whole-genome sequencing showed that the <italic>Aeromonas</italic> spp. isolates comprised <italic>A. caviae</italic> (<italic>n</italic> = 21), <italic>A. veronii</italic> (<italic>n</italic> = 18), <italic>A. dhakensis</italic> (<italic>n</italic> = 8), <italic>A. hydrophila</italic> (<italic>n</italic> = 7), <italic>A. jandaei</italic> (<italic>n</italic> = 5), <italic>A. enteropelogenes</italic> (<italic>n</italic> = 2), and <italic>A. media</italic> (<italic>n</italic> = 1). Using the whole-genome sequencing results as the standard, the consistency of the other methods was compared with them. The results were 46.77% (29/62) for biochemical identification, 83.87% (52/62) for mass spectrometric identification, 67.74% (42/62) for multiplex-PCR, 100% (62/62) for MLPA typing, 72.58% for <italic>gyrA</italic>, and 59.68% for <italic>rpoD</italic> and <italic>gyrA</italic>-<italic>rpoD</italic>. MLPA was the most consistent, followed by mass spectrometry. Therefore, in the public health laboratory, both MLPA and whole-genome sequencing methods can be used to identify various <italic>Aeromonas</italic> species. However, rapid and relatively accurate mass spectrometry is recommended for clinical lab.</p>