AUTHOR=Zhao Yuemeng , Wang Fei , Wang Changhong , Zhang Xiaobai , Jiang Cizhong , Ding Feng , Shen Li , Zhang Qingfeng TITLE=Optimization of CRISPR/Cas System for Improving Genome Editing Efficiency in Plasmodium falciparum JOURNAL=Frontiers in Microbiology VOLUME=11 YEAR=2021 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.625862 DOI=10.3389/fmicb.2020.625862 ISSN=1664-302X ABSTRACT=
Studies of molecular mechanisms and related gene functions have long been restricted by limited genome editing technologies in malaria parasites. Recently, a simple and effective genome editing technology, the CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system, has greatly facilitated these studies in many organisms, including malaria parasites. However, due to the special genome feature of malaria parasites, the manipulation and gene editing efficacy of the CRISPR/Cas system in this pathogen need to be improved, particularly in the human malaria parasite,