AUTHOR=Wang Wenbin , Liu Jianxin , Guo Shanshan , Liu Lei , Yuan Qianyun , Guo Lei , Pan Saikun 

TITLE=Identification of Vibrio parahaemolyticus and Vibrio spp. Specific Outer Membrane Proteins by Reverse Vaccinology and Surface Proteome

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.625315

DOI=10.3389/fmicb.2020.625315

ISSN=1664-302X

ABSTRACT=<p>The discovery of outer membrane proteins (OMPs) with desirable specificity and surface availability is a fundamental challenge to develop accurate immunodiagnostic assay and multivalent vaccine of pathogenic <italic>Vibrio</italic> species in food and aquaculture. Herein 101 OMPs were systemically screened from 4,831 non-redundant proteins of <italic>Vibrio parahaemolyticus</italic> by bioinformatical predication of signaling peptides, transmembrane (TM) α-helix, and subcellular location. The sequence homology analysis with 32 species of <italic>Vibrio</italic> spp. and all the non-<italic>Vibrio</italic> strains revealed that 15 OMPs were conserved in at least 23 <italic>Vibrio</italic> species, including BamA (VP2310), GspD (VP0133), Tolc (VP0425), OmpK (VP2362), OmpW (VPA0096), LptD (VP0339), Pal (VP1061), flagellar L-ring protein (VP0782), flagellar protein MotY (VP2111), hypothetical protein (VP1713), fimbrial assembly protein (VP2746), VacJ lipoprotein (VP2214), agglutination protein (VP1634), and lipoprotein (VP1267), Chitobiase (VP0755); high adhesion probability of flgH, LptD, OmpK, and OmpW indicated they were potential multivalent <italic>Vibrio</italic> vaccine candidates. <italic>V. parahaemolyticus</italic> OMPs were found to share high homology with at least one or two <italic>Vibrio</italic> species, 19 OMPs including OmpA like protein (VPA073), CsuD (VPA1504), and MtrC (VP1220) were found relatively specific to <italic>V. parahaemolyticus</italic>. The surface proteomic study by enzymatical shaving the cells showed the capsular polysaccharides most likely limited the protease action, while the glycosidases improved the availability of OMPs to trypsin. The OmpA (VPA1186, VPA0248, VP0764), Omp (VPA0166), OmpU (VP2467), BamA (VP2310), TolC (VP0425), GspD (VP0133), OmpK (VP2362), lpp (VPA1469), Pal (VP1061), agglutination protein (VP1634), and putative iron (III) compound receptor (VPA1435) have better availability on the cell surface.</p>