AUTHOR=Wang Yi , Wang Chen , Rajaofera Mamy Jayne Nelly , Zhu Li , Xu Xinze , Liu Wenbo , Zheng Fucong , Miao Weiguo 

TITLE=WY195, a New Inducible Promoter From the Rubber Powdery Mildew Pathogen, Can Be Used as an Excellent Tool for Genetic Engineering

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.610252

DOI=10.3389/fmicb.2020.610252

ISSN=1664-302X

ABSTRACT=<p>Until now, there are few studies and reports on the use of endogenous promoters of obligate biotrophic fungi. The WY195 promoter in the genome of <italic>Oidium heveae</italic>, the rubber powdery mildew pathogen, was predicted using PromoterScan and its promoter function was verified by the transient expression of the β-<italic>glucuronidase</italic> (<italic>GUS</italic>) gene. WY195 drove high levels of <italic>GUS</italic> expression in dicotyledons and monocotyledons. qRT-PCR indicated that <italic>GUS</italic> expression regulated by the WY195 promoter was 17.54-fold greater than that obtained using the CaMV 35S promoter in dicotyledons (<italic>Nicotiana tabacum</italic>), and 5.09-fold greater than that obtained using the ACT1 promoter in monocotyledons (<italic>Oryza sativa</italic>). Furthermore, WY195-regulated <italic>GUS</italic> gene expression was induced under high-temperature and drought conditions. Soluble proteins extracted from WY195-<italic>hpaXm</italic> transgenic tobacco was bioactive. Defensive micro-HR induced by the transgene expression of <italic>hpaXm</italic> was observed on transgenic tobacco leaves. Disease resistance bioassays showed that WY195-<italic>hpaXm</italic> transgenic tobacco enhanced the resistance to tobacco mosaic virus (TMV). WY195 has great potential for development as a new tool for genetic engineering. Further in-depth studies will help to better understand the transcriptional regulation mechanisms and the pathogenic mechanisms of <italic>O. heveae</italic>.</p>