AUTHOR=Li Jiawen , Li Ziwei , Zhao Tong , Yan Xiufeng , Pang Qiuying 

TITLE=Proteomic Analysis of Auricularia auricula-judae Under Freezing Treatment Revealed Proteins and Pathways Associated With Melanin Reduction

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.610173

DOI=10.3389/fmicb.2020.610173

ISSN=1664-302X

ABSTRACT=<p><italic>Auricularia auricula-judae</italic> is an edible nutrient-rich mushroom, which is a traditional medicinal resource in China. It is known that environment stimuli will affect the production of melanin by <italic>A. auricula-judae</italic>, but the mechanism of the effects of freezing treatment on melanin accumulation remains unknown. In the present study, the synthesis of melanin in <italic>A. auricula-judae</italic> was analyzed by physiological assays and a proteomics approach. Our findings showed that a longer freezing treatment causes a lighter color of <italic>A. auricula-judae</italic> fruiting bodies. The proteomic analysis showed that proteins involved in glycolysis/gluconeogenesis, tyrosine metabolism, ribosome, and arginine biosynthesis might contribute to the color differences in the <italic>A. auricula-judae</italic> after freezing treatment. This work will be expected to provide valuable information on the physiological and molecular mechanisms of freezing treatment on the color quality of <italic>A. auricula-judae</italic>.</p>