AUTHOR=Huang Hui , Qi Minbo , Liu Yiming , Wang Haixia , Wang Xuejun , Qiu Yiyang , Lu Zhenmei 

TITLE=Thiamine-Mediated Cooperation Between Auxotrophic Rhodococcus ruber ZM07 and Escherichia coli K12 Drives Efficient Tetrahydrofuran Degradation

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.594052

DOI=10.3389/fmicb.2020.594052

ISSN=1664-302X

ABSTRACT=<p>Tetrahydrofuran (THF) is a universal solvent widely used in the synthesis of chemicals and pharmaceuticals. As a refractory organic contaminant, it can only be degraded by a small group of microbes. In this study, a thiamine auxotrophic THF-degrading bacterium, <italic>Rhodococcus ruber</italic> ZM07, was isolated from an enrichment culture H-1. It was cocultured with <italic>Escherichia coli</italic> K12 (which cannot degrade THF but can produce thiamine) and/or <italic>Escherichia coli</italic> K12Δ<italic>thiE</italic> (which can neither degrade THF nor produce thiamine) with or without exogenous thiamine. This study aims to understand the interaction mechanisms between ZM07 and K12. We found that K12 accounted for 30% of the total when cocultured and transferred with ZM07 in thiamine-free systems; in addition, in the three-strain (ZM07, K12, and K12Δ<italic>thiE</italic>) cocultured system without thiamine, K12Δ<italic>thiE</italic> disappeared in the 8th transfer, while K12 could still stably exist (the relative abundance remained at approximately 30%). The growth of K12 was significantly inhibited in the thiamine-rich system. Its proportion was almost below 4% after the fourth transfer in both the two-strain (ZM07 and K12) and three-strain (ZM07, K12, and K12Δ<italic>thiE</italic>) systems; K12Δ<italic>thiE</italic>’s percentage was higher than K12’s in the three-strain (ZM07, K12, and K12Δ<italic>thiE</italic>) cocultured system with exogenous thiamine, and both represented only a small proportion (less than 1% by the fourth transfer). The results of the coculture of K12 and K12Δ<italic>thiE</italic> in thiamine-free medium indicated that intraspecific competition between them may be one of the main reasons for the extinction of K12Δ<italic>thiE</italic> in the three-strain (ZM07, K12, and K12Δ<italic>thiE</italic>) system without exogenous thiamine. Furthermore, we found that ZM07 could cooperate with K12 through extracellular metabolites exchanges without physical contact. This study provides novel insight into how microbes cooperate and compete with one another during THF degradation.</p>