AUTHOR=Sedrakyan Anahit M. , Ktsoyan Zhanna A. , Arakelova Karine A. , Zakharyan Magdalina K. , Hovhannisyan Alvard I. , Gevorgyan Zaruhi U. , Mnatsakanyan Armine A. , Kakabadze Elene G. , Makalatia Khatuna B. , Chanishvili Nina A. , Pirnay Jean-Paul , Arakelyan Arsen A. , Aminov Rustam I. 

TITLE=Extended-Spectrum β-Lactamases in Human Isolates of Multidrug-Resistant Non-typhoidal Salmonella enterica

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.592223

DOI=10.3389/fmicb.2020.592223

ISSN=1664-302X

ABSTRACT=<p>A total of 291 non-duplicate isolates of non-typhoidal <italic>Salmonella</italic> (NTS) were collected from the fecal samples of patients with salmonellosis in Armenia and Georgia during 1996–2016. The isolates were tested for resistance to antimicrobials, including extended-spectrum β-lactamases (ESBL). The high prevalence of multidrug-resistance (MDR) and ESBL-producer phenotypes was detected among <italic>Salmonella enterica</italic> subsp. <italic>enterica</italic> serovar Typhimurium (<italic>S</italic>. Typhimurium) isolates collected from patients in Armenia between 1996 and 2016. A total of 36 MDR NTS isolates were subjected to whole genome sequencing (WGS) to determine the genetic background of antimicrobial resistance (AMR) and mobile genetic elements. All ESBL-producing <italic>S</italic>. Typhimurium isolates belonged to the same sequence type (ST328). The ESBL-producer phenotype was associated with plasmid-encoded CTX-M-5 production. A range of other plasmids was associated with resistance to other antimicrobials, including the MDR phenotype.</p>