AUTHOR=Du Jiao , Song Xiao-yu , Shi Xiao-bin , Tang Xin , Chen Jian-bin , Zhang Zhan-hong , Chen Gong , Zhang Zhuo , Zhou Xu-guo , Liu Yong , Zhang De-yong 

TITLE=NSs, the Silencing Suppressor of Tomato Spotted Wilt Orthotospovirus, Interferes With JA-Regulated Host Terpenoids Expression to Attract Frankliniella occidentalis

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.590451

DOI=10.3389/fmicb.2020.590451

ISSN=1664-302X

ABSTRACT=<p>Tomato spotted wilt orthotospovirus (TSWV) causes serious crop losses worldwide and is transmitted by <italic>Frankliniella occidentalis</italic> (Pergande) (Thysanoptera: Thripidae). NSs protein is the silencing suppressor of TSWV and plays an important role in virus infection, cycling, and transmission process. In this research, we investigated the influences of NSs protein on the interaction of TSWV, plants, and <italic>F. occidentalis</italic> with the transgenic <italic>Arabidopsis thaliana</italic>. Compared with the wild-type Col-0 plant, <italic>F. occidentalis</italic> showed an increased number and induced feeding behavior on transgenic <italic>Arabidopsis thaliana</italic> expressing exogenous NSs. Further analysis showed that NSs reduced the expression of terpenoids synthesis-related genes and the content of monoterpene volatiles in <italic>Arabidopsis</italic>. These monoterpene volatiles played a repellent role in respect to <italic>F. occidentalis</italic>. In addition, the expression level of plant immune-related genes and the content of the plant resistance hormone jasmonic acid (JA) in transgenic <italic>Arabidopsis</italic> were reduced. The silencing suppressor of TSWV NSs alters the emission of plant volatiles and reduces the JA-regulated plant defenses, resulting in enhanced attractiveness of plants to <italic>F. occidentalis</italic> and may increase the transmission probability of TSWV.</p>