AUTHOR=Akter Nahid , Hashim Roshada , Pham Huy Quang , Choi Seung-Dae , Lee Dong-Woo , Shin Jae-Ho , Rajagopal Kammara 

TITLE=Lactobacillus acidophilus Antimicrobial Peptide Is Antagonistic to Aeromonas hydrophila

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.570851

DOI=10.3389/fmicb.2020.570851

ISSN=1664-302X

ABSTRACT=<p>We identified an antimicrobial peptide (AMP) from <italic>Lactobacillus acidophilus</italic> that was antagonistic to <italic>Aeromonas hydrophila</italic>. <italic>In vitro</italic> studies such as well-diffusion and field trials revealed that the AMP was active against <italic>A. hydrophila</italic>. The field trials of AMP using <italic>A. hydrophila</italic>-infected <italic>Channa striatus</italic> with a mannone oligosaccharide (MOS) prebiotic, <italic>A. hydrophila</italic> antigens, <italic>A. hydrophila</italic>-infected fish serum, <italic>L. acidophilus</italic>, and <italic>Lactobacillus</italic> cell free-supernatant (LABS-CFS) on an indicator organism further revealed that the antimicrobial agent could protect <italic>C</italic>. <italic>striatus</italic>. Other than the AMP, none of the above were able to eliminate the infectious agent <italic>A. hydrophila</italic>, and were only able to delay the death rate for 3–4 days. Thus, we conclude that the AMP is antagonistic to <italic>A. hydrophila</italic> and may be used for treatment of <italic>A. hydrophila</italic> infections. Subsequent <italic>L. acidophilus</italic> whole-genome sequence analyses enabled an understanding of the (probable) gene arrangement and its location on the chromosome. This information may be useful in the generation of recombinant peptides to produce larger quantities for treatment.</p>