AUTHOR=Meng Fanzeng , Zhu Tengfei , Yao Hao , Ling Zhiting , Feng Youwei , Li Guo , Li Jing , Sun Xinyu , Chen Jiaqi , Meng Chuang , Jiao Xin’an , Yin Yuelan 

TITLE=A Cross-Protective Vaccine Against 4b and 1/2b Listeria monocytogenes

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.569544

DOI=10.3389/fmicb.2020.569544

ISSN=1664-302X

ABSTRACT=<p><italic>Listeria monocytogenes</italic> (<italic>Lm</italic>) is a foodborne zoonotic pathogen that causes listeriosis with a mortality rate of 20–30%. Serovar 4b and 1/2b isolates account for most of listeriosis outbreaks, however, no listeriosis vaccine is available for either prophylactic or therapeutic use. Here, we developed a triple-virulence-genes deletion vaccine strain, and evaluated its safety, immunogenicity, and cross-protective efficiency. The virulence of NTSNΔ<italic>actA</italic>/<italic>plcB</italic>/<italic>orfX</italic> was reduced 794-folds compared with the parental strain. Additionally, it was completely eliminated in mice at day 7 post infection and no obvious pathological changes were observed in the organs of mice after prime-boost immunization for 23 days. These results proved that the safety of the <italic>Lm</italic> vaccine strain remarkably increased. More importantly, the NTSNΔ<italic>actA</italic>/<italic>plcB</italic>/<italic>orfX</italic> strain stimulated higher anti-Listeriolysin O (LLO) antibodies, induced significantly higher expression of IFN-γ, TNF-α, IL-17, and IL-6 than the control group, and afforded 100% protection against serovar 4b and 1/2b challenges. Taken together, our research demonstrates that the triple-genes-deletion vaccine has high safety, can elicit strong Th1 type immune response, and affords efficient cross-protection against two serovar <italic>Lm</italic> strains. It is a promising vaccine for prevention of listeriosis.</p>