AUTHOR=Ushijima Blake , Meyer Julie L. , Thompson Sharon , Pitts Kelly , Marusich Michael F. , Tittl Jessica , Weatherup Elizabeth , Reu Jacqueline , Wetzell Raquel , Aeby Greta S. , Häse Claudia C. , Paul Valerie J. 

TITLE=Disease Diagnostics and Potential Coinfections by Vibrio coralliilyticus During an Ongoing Coral Disease Outbreak in Florida

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.569354

DOI=10.3389/fmicb.2020.569354

ISSN=1664-302X

ABSTRACT=<p>A deadly coral disease outbreak has been devastating the Florida Reef Tract since 2014. This disease, stony coral tissue loss disease (SCTLD), affects at least 22 coral species causing the progressive destruction of tissue. The etiological agents responsible for SCTLD are unidentified, but pathogenic bacteria are suspected. Virulence screens of 400 isolates identified four potentially pathogenic strains of <italic>Vibrio</italic> spp. subsequently identified as <italic>V. coralliilyticus</italic>. Strains of this species are known coral pathogens; however, cultures were unable to consistently elicit tissue loss, suggesting an opportunistic role. Using an improved immunoassay, the VcpA <italic>RapidTest</italic>, a toxic zinc-metalloprotease produced by <italic>V. coralliilyticus</italic> was detected on 22.3% of diseased <italic>Montastraea cavernosa</italic> (<italic>n</italic> = 67) and 23.5% of diseased <italic>Orbicella faveolata</italic> (<italic>n</italic> = 24). VcpA<sup>+</sup> corals had significantly higher mortality rates and faster disease progression. For VcpA<sup>–</sup> fragments, 21.6% and 33.3% of <italic>M. cavernosa</italic> and <italic>O. faveolata</italic>, respectively, died within 21 d of observation, while 100% of similarly sized VcpA<sup>+</sup> fragments of both species died during the same period. Further physiological and genomic analysis found no apparent differences between the Atlantic <italic>V. coralliilyticus</italic> strains cultured here and pathogens from the Indo-Pacific but highlighted the diversity among strains and their immense genetic potential. In all, <italic>V. coralliilyticus</italic> may be causing coinfections that exacerbate existing SCTLD lesions, which could contribute to the intraspecific differences observed between colonies. This study describes potential coinfections contributing to SCTLD virulence as well as diagnostic tools capable of tracking the pathogen involved, which are important contributions to the management and understanding of SCTLD.</p>