AUTHOR=Wu Je-Jia , Huang Jenn-Wen , Deng Wen-Ling 

TITLE=Phenylacetic Acid and Methylphenyl Acetate From the Biocontrol Bacterium Bacillus mycoides BM02 Suppress Spore Germination in Fusarium oxysporum f. sp. lycopersici

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.569263

DOI=10.3389/fmicb.2020.569263

ISSN=1664-302X

ABSTRACT=<p><italic>Bacillus mycoides</italic> strain BM02 originally isolated from the tomato rhizosphere was found to have beneficial functions in tomato by promoting plant growth and reducing the severity of <italic>Fusarium</italic> wilt caused by <italic>Fusarium oxysporum</italic> f. sp. <italic>lycopersici</italic> (Fol). Cytological experiments demonstrated <italic>B. mycoides</italic> BM02 reduced Fol invasion by reducing spore attachment and increasing hyphal deformation in hydroponics-grown tomato root tissues. Two volatile antifungal compounds, phenylacetic acid (PAA) and methylphenyl acetate (MPA), were identified from the culture filtrates of <italic>B. mycoides</italic> BM02 by GC-MS analysis. Chemically synthesized PAA, and to a lower extent MPA, suppressed spore germination but have no effects on the hyphal growth of Fol. Our results indicated that the biocontrol agent <italic>B. mycoides</italic> BM02 produced an array of bioactive compounds including PAA and MPA to suppress plant diseases caused by Fol and other pathogenic microorganisms.</p>