AUTHOR=Peng Xueling , Yu Qilin , Liu Yingzheng , Ma Tianyu , Li Mingchun 

TITLE=Study on the Function of the Inositol Polyphosphate Kinases Kcs1 and Vip1 of Candida albicans in Energy Metabolism

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.566069

DOI=10.3389/fmicb.2020.566069

ISSN=1664-302X

ABSTRACT=<p>In <italic>Saccharomyces cerevisiae</italic>, inositol polyphosphate kinase <italic>KCS1</italic> but not <italic>VIP1</italic> knockout is of great significance for maintaining cell viability, promoting glycolysis metabolism, and inducing mitochondrial damage. The functions of <italic>Candida albicans</italic> inositol polyphosphate kinases Kcs1 and Vip1 have not yet been studied. In this study, we found that the growth rate of <italic>C. albicans vip1</italic>Δ/Δ strain in glucose medium was reduced and the upregulation of glycolysis was accompanied by a decrease in mitochondrial activity, resulting in a large accumulation of lipid droplets, along with an increase in cell wall chitin and cell membrane permeability, eventually leading to cell death. Relieving intracellular glycolysis rate or increasing mitochondrial metabolism can reduce lipid droplet accumulation, causing a reduction in chitin content and cell membrane permeability. The growth activity and energy metabolism of the <italic>vip1</italic>Δ/Δ strains in a non-fermentable carbon source glycerol medium were not different from those of the wild-type strains, indicating that knocking out <italic>VIP1</italic> did not cause mitochondria damage. Moreover, <italic>C. albicans KCS1</italic> knockout did not affect cell activity and energy metabolism. Thus, in <italic>C. albicans</italic>, Vip1 is more important than Kcs1 in regulating cell viability and energy metabolism.</p>