AUTHOR=Zuo Lingli , Zhou Liting , Wu Chaoyi , Wang Yanlin , Li Yuanyuan , Huang Rui , Wu Shuyan 

TITLE=Salmonella spvC Gene Inhibits Pyroptosis and Intestinal Inflammation to Aggravate Systemic Infection in Mice

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.562491

DOI=10.3389/fmicb.2020.562491

ISSN=1664-302X

ABSTRACT=<p><italic>Salmonella enterica</italic> serovar Typhimurium (<italic>S</italic>). Typhimurium is a primary foodborne pathogen infecting both humans and animals. <italic>Salmonella</italic> plasmid virulence C (<italic>spvC</italic>) gene is closely related to <italic>S</italic>. Typhimurium dissemination in mice, while the mechanisms remain to be fully elucidated. Pyroptosis, a gasdermin-mediated inflammatory cell death, plays a role in host defense against bacterial infection, whereas the effect of <italic>spvC</italic> on pyroptosis and its function in inflammatory injury induced by <italic>S</italic>. Typhimurium are rather limited. In our study, C57BL/6 mice and J774A.1 cells infected with <italic>S</italic>. Typhimurium wild-type strain SL1344, <italic>spvC</italic> deletion mutant, <italic>spvC</italic> K136A site-directed mutant, and complemented strain were used to investigate potential pathogenesis of <italic>spvC</italic>. We verity that SpvC attenuates intestinal inflammation, suppresses pyroptosis through phosphothreonine lyase activity, and reduces pyroptosis in the ceca. Moreover, the reduction of inflammation via <italic>spvC</italic> results in systemic infection. These findings demonstrate that <italic>spvC</italic> inhibits pyroptosis and intestinal inflammation to promote bacterial dissemination, which provide new strategies for controlling systemic infection caused by <italic>Salmonella</italic> and novel insights for the treatment of other corresponding diseases.</p>