AUTHOR=Wei Xingxing , Chen Lin , Tang Jian-Wei , Matsuda Yudai 

TITLE=Discovery of Pyranoviolin A and Its Biosynthetic Gene Cluster in Aspergillus violaceofuscus

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.562063

DOI=10.3389/fmicb.2020.562063

ISSN=1664-302X

ABSTRACT=<p>A new polyketide-non-ribosomal peptide hybrid molecule, pyranoviolin A (<bold>1</bold>), was discovered from the genome-sequenced fungus <italic>Aspergillus violaceofuscus</italic> CBS 115571 and was characterized to be the first pyranonigrin analog harboring the C-3 methoxy group. Examination of the genome sequence of the fungus identified a putative biosynthetic gene cluster of <bold>1</bold>, which was designated as the <italic>pyv</italic> cluster. The gene deletion experiment of the polyketide synthase (PKS)-non-ribosomal peptide synthetase (NRPS) hybrid gene in the cluster confirmed the involvement of the <italic>pyv</italic> cluster in the pyranoviolin A biosynthesis. Finally, a plausible biosynthetic route leading to <bold>1</bold> has been proposed based on the bioinformatic analysis. Our study indicates that metabolite analysis of genome-sequenced microorganisms whose metabolites have been largely unexplored facilitates the discovery of new secondary metabolites along with their biosynthetic gene clusters.</p>