AUTHOR=Dongzhen Fang , Xilin Liu , Xiaorong Chen , Wenwu Yan , Yunlu He , Yi Cheng , Jia Chen , Zhimin Li , Litao Guo , Tuhong Wang , Xu Jianping , Chunsheng Gao 

TITLE=Fusarium Species and Fusarium oxysporum Species Complex Genotypes Associated With Yam Wilt in South-Central China

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01964

DOI=10.3389/fmicb.2020.01964

ISSN=1664-302X

ABSTRACT=<p>Chinese yam (<italic>Dioscorea polystachya</italic> Thunb.) is an important root crop. Wilt caused by <italic>Fusarium</italic> is among the most important emerging diseases on yams. However, there is currently limited information on the molecular epidemiology of <italic>Fusarium</italic> causing yam wilt. Here, we investigated wilted yam samples from six regions in South-Central China. A total of 117 <italic>Fusarium</italic> isolates were obtained from diseased tissues of 37 wilted yam plants. These yam plants belonged to two varieties characterized by white and purple fleshy tubers, respectively. Analyses of <italic>ef1-α</italic> sequences identified that these 117 <italic>Fusarium</italic> isolates belonged to 11 putative species, with <italic>F. aff. commune</italic> being the most common (31.6%), followed by <italic>F. aff. cugenangense</italic> (29.1%), a potential undescribed species <italic>Fusarium aff</italic>. sp. (11.1%), <italic>F. aff. gossypinum</italic> (9.4%), <italic>F. aff. fujikuroi</italic> (8.5%), <italic>F. aff. nirenbergiae</italic> (6%), and one isolate each (0.85%) of <italic>F. aff. asiaticum</italic>, <italic>F. aff. curvatum</italic>, <italic>F. aff. odoratissimum, F. aff. solani</italic>, and <italic>F. aff. verticillioides</italic>. Six of these species were recently described as new species within the <italic>Fusarium oxysporum</italic> species complex (FOSC). Interestingly, 18 of the 37 yam plants were infected by two or more <italic>Fusarium</italic> species each and there was evidence for differential <italic>Fusarium</italic> species distributions based on geographic location and/or yam host variety. Multilocus microsatellite genotyping of the 67 FOSC isolates revealed that isolates of the same species from the same diseased plants often belonged to different genotypes. Interestingly, several FOSC microsatellite genotypes were shared among distinct geographic regions, consistent with long-distance dispersal. However, population genetic analyses revealed significant contributions of geographic separation to the overall genetic variation of FOSC with several pairs of geographic populations showing significant genetic differentiations, consistent with differential geographic distribution of the species within FOSC. The implications of our results to the managements of <italic>Fusarium</italic> wilt in yams were discussed.</p>