AUTHOR=Hosseini Somaieh , Amini Jahanshir , Saba Mahmoud Koushesh , Karimi Kaivan , Pertot Ilaria 

TITLE=Preharvest and Postharvest Application of Garlic and Rosemary Essential Oils for Controlling Anthracnose and Quality Assessment of Strawberry Fruit During Cold Storage

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01855

DOI=10.3389/fmicb.2020.01855

ISSN=1664-302X

ABSTRACT=<p>This study assessed the feasibility of using essential oils (EOs) against <italic>Colletotrichum nymphaeae</italic> inciting strawberry anthracnose. Two EOs, extracted from <italic>Allium sativum</italic> (garlic) and <italic>Rosmarinus officinalis</italic> (rosemary), were selected because their fungicide efficacy was already well characterized under laboratory and greenhouse conditions. We characterized both EOs in terms of efficacy and impact on qualitative traits and sensory quality of strawberry fruit. The gas chromatography–mass spectrometry analysis confirmed the Diallyl trisulfide (29.08%) and (α)-pinene (15.779%) as the main components of <italic>A. sativum</italic> and <italic>R. officinalis</italic> EOs, respectively. Both <italic>A. sativum</italic> and <italic>R. officinalis</italic> EOs significantly inhibited the mycelial growth and conidial germination of <italic>C. nymphaeae</italic> in contact and vapor assays compared with untreated control. However, EC<sub>50</sub> assay indicated <italic>A. sativum</italic> EO was more effective than <italic>R. officinalis</italic> EO against the pathogen. Malformations of the vegetative structures of the pathogen exposed to both EOs were revealed as shriveled, collapsed, and swelling mycelia in the cultures. Both EOs confirmed their efficacy under <italic>in vivo</italic> and greenhouse conditions; in fact, they significantly reduced the development of fruit decay and anthracnose disease incidence and severity, compared with untreated controls. Both EOs preserved sensory attributes and quality parameters of strawberry fruit including firmness, total soluble solids, ascorbic acid, antioxidant activity, and anthocyanin, but may leave unwanted smells. These findings suggest that two EOs can potentially represent an alternative to synthetic chemical fungicides against <italic>C. nymphaeae</italic> preserving fruit quality factors, although their cost and the impact on the fruit odor must be carefully taken into consideration before developing a commercial product.</p>