AUTHOR=Petito Guilherme , de Curcio Juliana Santana , Pereira Maristela , Bailão Alexandre Melo , Paccez Juliano Domiraci , Tristão Gabriel Brum , de Morais Camila Oliveira Barbosa , de Souza Marcelo Valle , de Castro Moreira Santos Agenor , Fontes Wagner , Ricart Carlos André Ornelas , de Almeida Soares Célia Maria 

TITLE=Metabolic Adaptation of Paracoccidioides brasiliensis in Response to in vitro Copper Deprivation

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01834

DOI=10.3389/fmicb.2020.01834

ISSN=1664-302X

ABSTRACT=<p>Copper is an essential micronutrient for the performance of important biochemical processes such as respiration detoxification, and uptake of metals like iron. Studies have shown that copper deprivation is a strategy used by the host against pathogenic fungi such as <italic>Cryptoccocus neoformans</italic> and <italic>Candida albicans</italic> during growth and development of infections in the lungs and kidneys. Although there are some studies, little is known about the impact of copper deprivation in members of the <italic>Paracoccidioides</italic> genus. Therefore, using isobaric tag labeling (iTRAQ)-Based proteomic approach and LC-MS/MS, we analyzed the impact of <italic>in vitro</italic> copper deprivation in the metabolism of <italic>Paracoccidioides brasiliensis</italic>. One hundred and sixty-four (164) differentially abundant proteins were identified when yeast cells were deprived of copper, which affected cellular respiration and detoxification processes. Changes in cellular metabolism such as increased beta oxidation and cell wall remodeling were described.</p>