AUTHOR=Kiefer Anthony , Tang Peipei , Arndt Samuel , Fallico Vincenzo , Wong Connie TITLE=Optimization of Viability Treatment Essential for Accurate Droplet Digital PCR Enumeration of Probiotics JOURNAL=Frontiers in Microbiology VOLUME=11 YEAR=2020 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01811 DOI=10.3389/fmicb.2020.01811 ISSN=1664-302X ABSTRACT=
Improvements offered by viability droplet digital PCR (v-ddPCR) include increased precision, specificity and decreased time to results making for an attractive alternative method to traditional plate count enumeration of probiotic products. A major hurdle faced in v-ddPCR, however, is distinguishing between live and dead cells. The objective of this study was to evaluate a combination of PMA and EMA (PE51) for viability treatment of freeze-dried probiotic powders.