AUTHOR=Clementi Nicola , Criscuolo Elena , Diotti Roberta Antonia , Ferrarese Roberto , Castelli Matteo , Dagna Lorenzo , Burioni Roberto , Clementi Massimo , Mancini Nicasio 

TITLE=Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01704

DOI=10.3389/fmicb.2020.01704

ISSN=1664-302X

ABSTRACT=<p>While the SARS-CoV-2 pandemic is heavily hitting the world, it is of extreme importance that significant <italic>in vitro</italic> observations guide the quick set up of clinical trials. In this study, we evidence that the anti-SARS-CoV2 activity of a clinically achievable hydroxychloroquine concentration is maximized only when administered before and after the infection of Vero E6 and Caco-2 cells. This suggests that only a combined prophylactic and therapeutic use of hydroxychloroquine may be effective in limiting viral replication in patients.</p>